mei-1 Meiotic spindle formation protein mei-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: mei-1
Official Full Name: Meiotic spindle formation protein mei-1
Primary source: WormBase:WBGene00003183
Locus tag: CELE_T01G9.5
See related: AllianceGenome:WB:WBGene00003183
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables several functions, including ATP binding activity; MATH domain binding activity; and microtubule severing ATPase activity. Involved in several processes, including cytoskeleton organization; female meiotic nuclear division; and negative regulation of meiotic spindle elongation. Located in chromatin; meiotic spindle pole; and nucleus. Part of katanin complex. Is expressed in ciliated neurons; germ line; and head neurons. Orthologous to human KATNA1 (katanin catalytic subunit A1) and KATNAL1 (katanin catalytic subunit A1 like 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See mei-1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 4

Sequence:: Chromosome: I; NC_003279.8 (8294874..8296940)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces.
Title: Katanin spiral and ring structures shed light on power stroke for microtubule severing.
mei-1 and aspm-1 promote oocyte meiotic spindle pole assembly
Title: Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1.
Purified MEI-1/MEI-2 complexes preferentially sever at intersections between two microtubules and directly bundle microtubules in vitro.
Title: Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro.
The phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis.
Title: Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation.
study demonstrates that the level of MEI-1 protein is reduced in an unc-89 mutant, indicating that the normal role of UNC-89 is to inhibit the CUL-3/MEL-26 complex toward MEI-1 in striated muscle
Title: UNC-89 (obscurin) binds to MEL-26, a BTB-domain protein, and affects the function of MEI-1 (katanin) in striated muscle of Caenorhabditis elegans.
MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets
Title: Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis.
MEI-1 is required for translocation of the meiosis I spindle to the oocyte cortex.
Title: MEI-1/katanin is required for translocation of the meiosis I spindle to the oocyte cortex in C elegans.
the identification of BTB-domain protein MEL-26 and its genetic target MEI-1 as substrate-specific adaptors for C. elegans CUL-3
Title: BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3.
The BTB-containing protein MEL-26 is a component required for degradation of MEI-1 in vivo; importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor
Title: The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T01G9.5 (e-PCR)
- UniSTS:310604

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables MATH domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule severing ATPase activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule severing ATPase activity	IEA Inferred from Electronic Annotation more info
enables microtubule severing ATPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables molecular adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic spindle disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule severing	IEA Inferred from Electronic Annotation more info
involved_in microtubule severing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of meiotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in striated muscle myosin thick filament assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of katanin complex	IDA Inferred from Direct Assay more info	PubMed
located_in meiotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in meiotic spindle pole	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Meiotic spindle formation protein mei-1

NP_001370150.1

Confirmed by transcript evidence

NP_492257.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

8294874..8296940

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001383460.2 → NP_001370150.1 Meiotic spindle formation protein mei-1 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (2) summary

pfam17862
Location:383 → 423

AAA_lid_3; AAA+ lid domain

cd19509
Location:195 → 359

RecA-like_VPS4-like; ATPase domain of VPS4, ATAD1, K, KTNA1, Spastin, FIGL-1 and similar ATPase domains
NM_059856.8 → NP_492257.1 Meiotic spindle formation protein mei-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_492257.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P34808, Q8I4G6

Conserved Domains (2) summary

pfam17862
Location:383 → 416

AAA_lid_3; AAA+ lid domain

cd19509
Location:195 → 359

RecA-like_VPS4-like; ATPase domain of VPS4, ATAD1, K, KTNA1, Spastin, FIGL-1 and similar ATPase domains