Ephx2 epoxide hydrolase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ephx2provided by RGD
Official Full Name: epoxide hydrolase 2provided by RGD
Primary source: RGD:620732
See related: EnsemblRapid:ENSRNOG00000017286 AllianceGenome:RGD:620732
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables epoxide hydrolase activity; magnesium ion binding activity; and phosphatase activity. Involved in several processes, including linoleic acid metabolic process; prostaglandin production involved in inflammatory response; and response to 3-methylcholanthrene. Located in peroxisome. Used to study cerebral infarction; hypertension; and pulmonary hypertension. Biomarker of hypertrophic cardiomyopathy. Human ortholog(s) of this gene implicated in coronary artery disease; familial hypercholesterolemia; and hypertension. Orthologous to human EPHX2 (epoxide hydrolase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 385.6), Liver (RPKM 266.1) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 15p12

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (44465447..44503157, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (40289901..40327632, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (42757241..42794211, complement)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role of soluble epoxide hydrolase in the abnormal activation of fibroblast-like synoviocytes from patients with rheumatoid arthritis.
Title: Role of soluble epoxide hydrolase in the abnormal activation of fibroblast-like synoviocytes from patients with rheumatoid arthritis.
Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPbeta.
Title: Soluble epoxide hydrolase and TRPC3 channels jointly contribute to homocysteine-induced cardiac hypertrophy: Interrelation and regulation by C/EBPβ.
CRISPR/Cas9-mediated inactivation of the phosphatase activity of soluble epoxide hydrolase prevents obesity and cardiac ischemic injury.
Title: CRISPR/Cas9-mediated inactivation of the phosphatase activity of soluble epoxide hydrolase prevents obesity and cardiac ischemic injury.
Soluble epoxide hydrolase inhibition improves cognitive function and parenchymal artery dilation in a hypertensive model of chronic cerebral hypoperfusion.
Title: Soluble epoxide hydrolase inhibition improves cognitive function and parenchymal artery dilation in a hypertensive model of chronic cerebral hypoperfusion.
Vascular smooth muscle expression of Nox4 and soluble epoxide hydrolase synergistically contribute to homocysteine-induced inflammation.
Title: Nox4 and soluble epoxide hydrolase synergistically mediate homocysteine-induced inflammation in vascular smooth muscle cells.
Study shows that inhibition of soluble epoxide hydrolase regulates monocyte/macrophage polarization and improves neurological outcome in a rat model of ischemic stroke
Title: Inhibition of soluble epoxide hydrolase regulates monocyte/macrophage polarization and improves neurological outcome in a rat model of ischemic stroke.
Daunorubicin induces cardiotoxicity through a sEH-mediated epoxyeicosatrienoic acids degradation-dependent mechanism.
Title: The Role of Soluble Epoxide Hydrolase Enzyme on Daunorubicin-Mediated Cardiotoxicity.
Inhibition of the sEH alleviates diabetes-induced decline in learning and memory.
Title: An inhibitor of soluble epoxide hydrolase ameliorates diabetes-induced learning and memory impairment in rats.
Data suggest that significant dietary potassium (here, even as a single meal) increases postprandial effects that reduce sEH activity in blood by mechanisms involving insulin secretion and gut microbiota; these mechanisms combat oxidative stress and prevent cardiovascular diseases.
Title: Postprandial effect to decrease soluble epoxide hydrolase activity: roles of insulin and gut microbiota.
Prophylactic treatment with sEH inhibitors decreased myocardial damage due to ischemia reperfusion, hypertension and diabetes, and decreased endothelial dysfunction created by diabetes and hypertension.
Title: Inhibitors of soluble epoxide hydrolase minimize ischemia-reperfusion-induced cardiac damage in normal, hypertensive, and diabetic rats.

Submit: New GeneRIF Correction See all GeneRIFs (23)

General gene information

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Markers

RH138659 (e-PCR)
- UniSTS:220746

D15Rat72 (e-PCR), detects polymorphism
- UniSTS:118415

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity	IEA Inferred from Electronic Annotation more info
enables epoxide hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables epoxide hydrolase activity	IEA Inferred from Electronic Annotation more info
enables epoxide hydrolase activity	ISO Inferred from Sequence Orthology more info
enables lipid phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables lipid phosphatase activity	IEA Inferred from Electronic Annotation more info
enables lipid phosphatase activity	ISO Inferred from Sequence Orthology more info
enables lipid phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables lysophosphatidic acid phosphatase activity	IEA Inferred from Electronic Annotation more info
enables lysophosphatidic acid phosphatase activity	ISO Inferred from Sequence Orthology more info
enables lysophosphatidic acid phosphatase activity	ISS Inferred from Sequence or Structural Similarity more info
enables magnesium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatase activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables toxic substance binding	IEA Inferred from Electronic Annotation more info
enables toxic substance binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cholesterol homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cholesterol homeostasis	IEA Inferred from Electronic Annotation more info
involved_in cholesterol homeostasis	ISO Inferred from Sequence Orthology more info
involved_in dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in epoxide metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in epoxide metabolic process	IEA Inferred from Electronic Annotation more info
involved_in epoxide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid dephosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid dephosphorylation	IEA Inferred from Electronic Annotation more info
involved_in phospholipid dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in phospholipid dephosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin production involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cholesterol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of cholesterol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to 3-methylcholanthrene	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to toxic substance	IEA Inferred from Electronic Annotation more info
involved_in stilbene catabolic process	IEA Inferred from Electronic Annotation more info
involved_in stilbene catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in peroxisome	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info
located_in peroxisome	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: bifunctional epoxide hydrolase 2

Names: CEH; SEH; cytosolic epoxide hydrolase; epoxide hydratase; epoxide hydrolase 2, cytoplasmic; soluble epoxide hydrolase

NP_075225.1

EC 3.1.3.76

EC 3.3.2.10

XP_006252209.1

EC 3.1.3.76

EC 3.3.2.10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022936.1 → NP_075225.1 bifunctional epoxide hydrolase 2

See identical proteins and their annotated locations for NP_075225.1

Status: PROVISIONAL

Source sequence(s)

X65083

UniProtKB/Swiss-Prot

P80299

UniProtKB/TrEMBL

A0A9K3Y813, D4A6V6, Q5RKK3

Related

ENSRNOP00000023385.6, ENSRNOT00000023385.9

Conserved Domains (4) summary

TIGR01509
Location:5 → 203

HAD-SF-IA-v3; haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

pfam00561
Location:257 → 530

Abhydrolase_1; alpha/beta hydrolase fold

cl21460
Location:142 → 205

HAD_like; Haloacid Dehalogenase-like Hydrolases

cl21494
Location:239 → 379

Abhydrolase; alpha/beta hydrolases

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086033.1 Reference GRCr8

Range

44465447..44503157 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006252147.4 → XP_006252209.1 bifunctional epoxide hydrolase 2 isoform X1

UniProtKB/TrEMBL

A6K6L7

Conserved Domains (4) summary

TIGR01509
Location:6 → 137

HAD-SF-IA-v3; haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

pfam00561
Location:191 → 464

Abhydrolase_1; alpha/beta hydrolase fold

cl21460
Location:76 → 139

HAD_like; Haloacid Dehalogenase-like Hydrolases

cl21494
Location:173 → 313

Abhydrolase; alpha/beta hydrolases