BRF1 transcription factor TFIIIB subunit BRF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BRF1
Official Full Name: transcription factor TFIIIB subunit BRF1
Primary source: SGD:S000003478
Locus tag: YGR246C
See related: AllianceGenome:SGD:S000003478; FungiDB:YGR246C; VEuPathDB:YGR246C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PCF4; TDS4
Summary: Enables DNA binding activity; RNA polymerase III core binding activity; and general transcription initiation factor binding activity. Involved in DNA-templated transcription open complex formation and RNA polymerase III preinitiation complex assembly. Part of transcription factor TFIIIB complex. Used to study cerebellofaciodental syndrome and colorectal cancer. Human ortholog(s) of this gene implicated in cerebellofaciodental syndrome. Orthologous to human BRF1 (BRF1 general transcription factor IIIB subunit). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See BRF1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (982482..984272, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation.
Title: The TFIIE-related Rpc82 subunit of RNA polymerase III interacts with the TFIIB-related transcription factor Brf1 and the polymerase cleft for transcription initiation.
Data indicate the binding between Brf1 cyclin repeats and the highly conserved region connecting C34 winged-helix domains 2 and 3.
Title: Mapping the protein interaction network for TFIIB-related factor Brf1 in the RNA polymerase III preinitiation complex.
A structural model of the interaction interface between transcription factor TFIIIB essential subunit (Bdp1) and Brf1 shows the main Bdp1 anchorage site provided by a small set of residues belonging to a Brf1 segment of residues 470-495.
Title: Structural characterization of the interaction between TFIIIB components Bdp1 and Brf1.
Entry of Brf1 and Bdp1 into the transcription complex imposes a strict sequence preference for the downstream half of the TATA box that matches the TATA box of the pol III-transcribed U6 small nuclear RNA gene.
Title: The Saccharomyces cerevisiae RNA polymerase III recruitment factor subunits Brf1 and Bdp1 impose a strict sequence preference for the downstream half of the TATA box.
The data are consistent with this peptide causing a conformational change in TFIIIC that overcomes Tfc4 autoinhibition of Brf1 binding and suggest a structural model for the Brf1-Tfc4 interaction.
Title: Interactions of Brf1 peptides with the tetratricopeptide repeat-containing subunit of TFIIIC inhibit and promote preinitiation complex assembly.
analysis of the interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB
Title: Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase III core binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase III core binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase III general transcription initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase III type 3 promoter sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables TBP-class protein binding	IDA Inferred from Direct Assay more info	PubMed
enables TBP-class protein binding	IEA Inferred from Electronic Annotation more info
enables TFIIIC-class transcription factor complex binding	IDA Inferred from Direct Assay more info	PubMed
enables TFIIIC-class transcription factor complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription open complex formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase III preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA polymerase III preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase III	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription preinitiation complex assembly	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIIB complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIIB complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: transcription factor TFIIIB subunit BRF1

NP_011762.1

TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

982482..984272 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181375.1 → NP_011762.1 TPA: transcription factor TFIIIB subunit BRF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011762.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV26, P29056

UniProtKB/TrEMBL

A6ZUQ4, C7GNI3, C8Z9D8, G2WEX2, N1P9Z1

Conserved Domains (2) summary

COG1405
Location:1 → 261

SUA7; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB [Transcription]

pfam07741
Location:463 → 595

BRF1; Brf1-like TBP-binding domain