RNR2 ribonucleotide-diphosphate reductase subunit RNR2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RNR2
Official Full Name: ribonucleotide-diphosphate reductase subunit RNR2
Primary source: SGD:S000003563
Locus tag: YJL026W
See related: AllianceGenome:SGD:S000003563; FungiDB:YJL026W; VEuPathDB:YJL026W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CRT6
Summary: Contributes to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor. Involved in deoxyribonucleotide biosynthetic process. Located in cytoplasm and nucleus. Part of ribonucleoside-diphosphate reductase complex. Human ortholog(s) of this gene implicated in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 5; mitochondrial DNA depletion syndrome 8a; mitochondrial DNA depletion syndrome 8b; and pancreatic cancer. Orthologous to human RRM2B (ribonucleotide reductase regulatory TP53 inducible subunit M2B) and RRM2 (ribonucleotide reductase regulatory subunit M2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RNR2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (392404..393603)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rnr2-Rnr4 redistribution in S phase is triggered by Clb6-Cdc28-mediated phosphorylation of Rnr2, which disrupts the Rnr2-Wtm1 interaction and promotes the release of Rnr2-Rnr4 from the nucleus.
Title: Clb6-Cdc28 Promotes Ribonucleotide Reductase Subcellular Redistribution during S Phase.
these results reveal that, in response to iron starvation, Dun1 kinase phosphorylates Dif1 to stimulate Rnr2-Rnr4 relocalization to the cytoplasm and promote RNR function.
Title: Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency.
This study characterized the surface of interaction between Hug1 and the small RNR subunit Rnr2.
Title: Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit.
diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.(
Title: Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.
Dif1 is required for nuclear import of the R2 subunit and plays an essential role in regulating the dynamic RNR subcellular localization.
Title: Dif1 controls subcellular localization of ribonucleotide reductase by mediating nuclear import of the R2 subunit.
Wtm1 acts either as an adaptor to facilitate nuclear import of betabeta'(RNR2) by Kap122 or as an anchor to retain betabeta' in the nucleus
Title: Nuclear localization of the Saccharomyces cerevisiae ribonucleotide reductase small subunit requires a karyopherin and a WD40 repeat protein.
Wtm1 acts as a nuclear anchor to maintain nuclear localization of Rnr2/4 complexes outside of S phase. In the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex.
Title: Control of ribonucleotide reductase localization through an anchoring mechanism involving Wtm1.
Rnr2 and Rnr4 are transported between the nucleus and the cytoplasm as one protein complex. Tagging either Rnr2 or Rnr4 with a nuclear export sequence causes cytoplasmic localization of both proteins.
Title: Cotransport of the heterodimeric small subunit of the Saccharomyces cerevisiae ribonucleotide reductase between the nucleus and the cytoplasm.
under normal growth conditions, the cell assembles stoichiometric amounts of Y(*) and modulation of Y(*) concentration is not involved in the regulation of RNR activity.
Title: Determination of the in vivo stoichiometry of tyrosyl radical per betabeta' in Saccharomyces cerevisiae ribonucleotide reductase.
studies demonstrate that beta' plays a crucial role in cluster assembly in vitro and in vivo and that the active form of the yeast R2 is betabeta'
Title: The active form of the Saccharomyces cerevisiae ribonucleotide reductase small subunit is a heterodimer in vitro and in vivo.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IDA Inferred from Direct Assay more info	PubMed
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IDA Inferred from Direct Assay more info	PubMed
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in deoxyribonucleotide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in deoxyribonucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoside-diphosphate reductase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ribonucleotide-diphosphate reductase subunit RNR2

NP_012508.1

EC 1.17.4.1

Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

392404..393603

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181460.1 → NP_012508.1 TPA: ribonucleotide-diphosphate reductase subunit RNR2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012508.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWF6, P09938

UniProtKB/TrEMBL

A6ZPU8, B3LQ83, C7GSQ1, G2WGZ0, N1NZY1

Conserved Domains (1) summary

cl00264
Location:76 → 399

Ferritin_like; Ferritin-like superfamily of diiron-containing four-helix-bundle proteins