Gss glutathione synthetase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gssprovided by MGI
Official Full Name: glutathione synthetaseprovided by MGI
Primary source: MGI:MGI:95852
See related: Ensembl:ENSMUSG00000027610 AllianceGenome:MGI:95852
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GSH-S; GS-A/GS-B
Summary: Enables glutathione synthase activity. Acts upstream of or within response to cadmium ion. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; integumental system; metanephros; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease; glutathione synthetase deficiency; glutathione synthetase deficiency of erythrocytes; and glutatione synthetase deficiency with 5-oxoprolinuria. Orthologous to human GSS (glutathione synthetase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in kidney adult (RPKM 72.6), colon adult (RPKM 41.7) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 77.26 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (155405101..155434730, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (155563181..155592810, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice.
Title: Gss deficiency causes age-related fertility impairment via ROS-triggered ferroptosis in the testes of mice.
Cystine/Glutamate Xc[-] Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis.
Title: Cystine/Glutamate Xc(-) Antiporter Induction Compensates for Transsulfuration Pathway Repression by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) to Ensure Cysteine for Hepatic Glutathione Biosynthesis.
the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase
Title: A futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase: the cause of cellular ATP depletion in nephrotic cystinosis?
Hypoxia decreased 2 key enzyme activities that regulate GSH synthesis, glutamate cysteine ligase (GCL) (E.C. 6.3.2.2) and glutathione synthase (GS) (E.C. 6.3.2.3)
Title: Hypoxia and kinase activity regulate lung epithelial cell glutathione.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI314904 (e-PCR)
- UniSTS:178754

Gss (e-PCR), detects polymorphism
- UniSTS:506956

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables glutathione binding	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione binding	ISO Inferred from Sequence Orthology more info
enables glutathione binding	ISS Inferred from Sequence or Structural Similarity more info
enables glutathione synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutathione synthase activity	ISO Inferred from Sequence Orthology more info
enables glycine binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables peptide binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in glutathione biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in glutathione biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to cadmium ion	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: glutathione synthetase

Names: GSH synthetase; glutathione synthase

NP_001278040.1

EC 6.3.2.3

NP_032206.1

EC 6.3.2.3

XP_006498844.1

EC 6.3.2.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001291111.1 → NP_001278040.1 glutathione synthetase isoform b

See identical proteins and their annotated locations for NP_001278040.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

Source sequence(s)

AI846976, AK149577, AL844852

Consensus CDS

CCDS71167.1

UniProtKB/TrEMBL

Q3UEE2, Q8R436

Related

ENSMUSP00000135319.2, ENSMUST00000130881.8

Conserved Domains (1) summary

cl00170
Location:1 → 405

eu-GS; Eukaryotic Glutathione Synthetase (eu-GS); catalyses the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Belongs to the ATP-grasp superfamily.
NM_008180.2 → NP_032206.1 glutathione synthetase isoform a

See identical proteins and their annotated locations for NP_032206.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

Source sequence(s)

AI846976, AK169096, AL844852

Consensus CDS

CCDS16951.1

UniProtKB/Swiss-Prot

P51855

UniProtKB/TrEMBL

Q3TWM2, Q541E2

Related

ENSMUSP00000078630.7, ENSMUST00000079691.13

Conserved Domains (1) summary

cd00228
Location:6 → 474

eu-GS; Eukaryotic Glutathione Synthetase (eu-GS); catalyses the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Belongs to the ATP-grasp superfamily.

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

155405101..155434730 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006498781.4 → XP_006498844.1 glutathione synthetase isoform X1

See identical proteins and their annotated locations for XP_006498844.1

UniProtKB/Swiss-Prot

P51855

UniProtKB/TrEMBL

Q3TWM2, Q541E2

Conserved Domains (1) summary

cd00228
Location:6 → 474

eu-GS; Eukaryotic Glutathione Synthetase (eu-GS); catalyses the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Belongs to the ATP-grasp superfamily.