BUB1 protein kinase BUB1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BUB1
Official Full Name: protein kinase BUB1
Primary source: SGD:S000003420
Locus tag: YGR188C
See related: AllianceGenome:SGD:S000003420; FungiDB:YGR188C; VEuPathDB:YGR188C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein kinase activity. Involved in several processes, including chromosome organization; mitotic spindle assembly checkpoint signaling; and protein localization to kinetochore. Located in kinetochore and nucleus. Human ortholog(s) of this gene implicated in colorectal cancer; mosaic variegated aneuploidy syndrome 1; and primary autosomal recessive microcephaly. Orthologous to several human genes including BUB1 (BUB1 mitotic checkpoint serine/threonine kinase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See BUB1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (872044..875109, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.
Title: Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.
The Bub1 TPR domain is both necessary and sufficient to bind and recruit Mad3.
Title: The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.
Bub1 mutant cells fail to maintain a prolonged checkpoint arrest in the presence of tensionless attachment.
Title: Premature Silencing of the Spindle Assembly Checkpoint Is Prevented by the Bub1-H2A-Sgo1-PP2A Axis in Saccharomyces cerevisiae.
Data indicate that spindle checkpoint factors Bub1 and Bub2 are recruited to the site of DNA damage.
Title: Spindle Checkpoint Factors Bub1 and Bub2 Promote DNA Double-Strand Break Repair by Nonhomologous End Joining.
Bub1 and Sgo1 act as a rheostat to regulate the chromatin spring and maintain force balance. Through histone H2A S121 phosphorylation and recruitment of Sgo1, Bub1 kinase softens the chromatin spring in response to changes in microtubule dynamics.
Title: Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics.
propose that Bub1, Sgo1, and Mps1 facilitate chromosome biorientation independently of the Aurora B-mediated pathway at the budding yeast kinetochore and that both pathways are required for the efficient turnover of syntelic attachments
Title: Bub1, Sgo1, and Mps1 mediate a distinct pathway for chromosome biorientation in budding yeast.
Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
Title: Bub1-mediated adaptation of the spindle checkpoint.
Bub1 kinase and Sgo1p act together to ensure efficient biorientation of sister chromatids during mitosis.
Title: Bub1 kinase targets Sgo1 to ensure efficient chromosome biorientation in budding yeast mitosis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in centromere complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic sister chromatid cohesion, centromeric	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sister chromatid biorientation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein kinase BUB1

NP_011704.3

EC 2.7.11.1

Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

872044..875109 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181317.3 → NP_011704.3 TPA: protein kinase BUB1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011704.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUX1, P41695

UniProtKB/TrEMBL

A6ZUJ9, B3LI47, B5VJD0, C8Z981, N1P2H0

Conserved Domains (3) summary

smart00777
Location:37 → 169

Mad3_BUB1_I; Mad3/BUB1 hoMad3/BUB1 homology region 1

cd13981
Location:704 → 1008

STKc_Bub1_BubR1; Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 and BubR1

pfam08171
Location:291 → 352

Mad3_BUB1_II; Mad3/BUB1 homology region 2