Slc22a3 solute carrier family 22 (organic cation transporter), member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc22a3provided by MGI
Official Full Name: solute carrier family 22 (organic cation transporter), member 3provided by MGI
Primary source: MGI:MGI:1333817
See related: Ensembl:ENSMUSG00000023828 AllianceGenome:MGI:1333817
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EMT; Oct3; Orct3; Slca22a3
Summary: Enables monoamine transmembrane transporter activity; neurotransmitter transmembrane transporter activity; and quaternary ammonium group transmembrane transporter activity. Involved in histamine transport and monoamine transport. Acts upstream of or within several processes, including histamine uptake; quaternary ammonium group transport; and regulation of appetite. Located in several cellular components, including apical plasma membrane; mitochondrial membrane; and neuronal cell body. Is expressed in several structures, including brain; dermis; extraembryonic component; genitourinary system; and thymus primordium. Orthologous to human SLC22A3 (solute carrier family 22 member 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in subcutaneous fat pad adult (RPKM 16.2), genital fat pad adult (RPKM 10.1) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A1; 17 8.52 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (12638859..12726591, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (12419972..12507704, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Induction of astrocytic Slc22a3 regulates sensory processing through histone serotonylation.
Title: Induction of astrocytic Slc22a3 regulates sensory processing through histone serotonylation.
Intracellular beta1-Adrenergic Receptors and Organic Cation Transporter 3 Mediate Phospholamban Phosphorylation to Enhance Cardiac Contractility.
Title: Intracellular β(1)-Adrenergic Receptors and Organic Cation Transporter 3 Mediate Phospholamban Phosphorylation to Enhance Cardiac Contractility.
Study is the first to examine the subcellular localization of organic cation transporter 3 (OCT3) in any tissue, and the first to explore its spatial relationship to synaptic structures in the brain. Study suggests that OCT3 not only contributes to the clearance of extracellular monoamines, but also plays a role in their intracellular disposition and action.
Title: Organic cation transporter 3 (OCT3) is localized to intracellular and surface membranes in select glial and neuronal cells within the basolateral amygdaloid complex of both rats and mice.
Metformin undergoes negligible metabolism, these results imply that intestinal absorption of metformin is mediated at least in part by Oct3 in mice.
Title: Involvement of organic cation transporter 3 (Oct3/Slc22a3) in the bioavailability and pharmacokinetics of antidiabetic metformin in mice.
Study showed that few, if any, 5-HT neurons in the dorsal raphe are resistant to loss of Pet-1 for expression of Slc22a3
Title: Pet-1 Controls Tetrahydrobiopterin Pathway and Slc22a3 Transporter Genes in Serotonin Neurons.
Findings suggest that OCT3 plays an important role in the absorption and elimination of metformin and that the transporter is a critical determinant of metformin bioavailability, clearance, and pharmacologic action.
Title: Targeted disruption of organic cation transporter 3 attenuates the pharmacologic response to metformin.
These results demonstrate that OCT3 is responsible for metformin accumulation and secretion in salivary glands.
Title: Taste of a pill: organic cation transporter-3 (OCT3) mediates metformin accumulation and secretion in salivary glands.
Data indicate that the expression of the organic cation transporters Slc22a1, Slc22a2 and Slc22a3 essential for the cellular uptake of metformin was highly suppressed in Tsc1+/-renal tumours.
Title: Renal tumours in a Tsc1+/- mouse model show epigenetic suppression of organic cation transporters Slc22a1, Slc22a2 and Slc22a3, and do not respond to metformin.
Transcripts encoding OCT1-3 and ChAT (choline acetyltransferase) have been detected in tracheal epithelial cells; these data suggest that cells lining the airway are able to synthesize/secrete acetylcholine into the lumen.
Title: Luminal cholinergic signalling in airway lining fluid: a novel mechanism for activating chloride secretion via Ca²⁺-dependent Cl⁻ and K⁺ channels.
evidence for a new means of downregulating IL-4 production by basophils, both in vitro and in vivo, through OCT3 targeted by 5-HT and pharmacologic ligands.
Title: Downregulation of basophil-derived IL-4 and in vivo T(H)2 IgE responses by serotonin and other organic cation transporter 3 ligands.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

General gene information

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Markers

Slc22a3 (e-PCR), detects polymorphism
- UniSTS:463370

RH132315 (e-PCR)
- UniSTS:215598

BE692283 (e-PCR)
- UniSTS:209270

D17Jcs121 (e-PCR), detects polymorphism
- UniSTS:259260

AI415450 (e-PCR)
- UniSTS:160234

35.MMHAP35FLD5.seq (e-PCR)
- UniSTS:124142

Slc22a3 (e-PCR), detects polymorphism
- UniSTS:547320

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables dopamine:sodium symporter activity	IEA Inferred from Electronic Annotation more info
enables dopamine:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables monoamine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables monoamine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables neurotransmitter transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables neurotransmitter transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables organic anion transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables organic anion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables organic cation transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables organic cation transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables organic cation transmembrane transporter activity	ISO Inferred from Sequence Orthology more info	PubMed
enables quaternary ammonium group transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables quaternary ammonium group transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables spermidine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables spermidine transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables toxin transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables toxin transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular detoxification	IEA Inferred from Electronic Annotation more info
involved_in cellular detoxification	ISO Inferred from Sequence Orthology more info
involved_in dopamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine transport	ISO Inferred from Sequence Orthology more info
involved_in dopamine uptake	IEA Inferred from Electronic Annotation more info
involved_in dopamine uptake	ISO Inferred from Sequence Orthology more info
involved_in epinephrine transport	IEA Inferred from Electronic Annotation more info
involved_in epinephrine transport	ISO Inferred from Sequence Orthology more info
involved_in epinephrine uptake	IEA Inferred from Electronic Annotation more info
involved_in epinephrine uptake	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within histamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histamine transport	ISO Inferred from Sequence Orthology more info
involved_in histamine transport	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within histamine uptake	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histamine uptake	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monoamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in monoamine transport	ISO Inferred from Sequence Orthology more info
involved_in monoatomic ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within monocarboxylic acid transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neurotransmitter transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter transport	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in norepinephrine transport	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in norepinephrine uptake	IEA Inferred from Electronic Annotation more info
involved_in norepinephrine uptake	ISO Inferred from Sequence Orthology more info
involved_in organic anion transport	ISO Inferred from Sequence Orthology more info
involved_in organic cation transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within organic cation transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within organic cation transport	ISO Inferred from Sequence Orthology more info	PubMed
involved_in organic cation transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within organic hydroxy compound transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within quaternary ammonium group transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within quaternary ammonium group transport	ISO Inferred from Sequence Orthology more info
involved_in quaternary ammonium group transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of appetite	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in serotonin transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in serotonin transport	ISO Inferred from Sequence Orthology more info
involved_in serotonin uptake	IEA Inferred from Electronic Annotation more info
involved_in serotonin uptake	ISO Inferred from Sequence Orthology more info
involved_in spermidine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in spermidine transport	ISO Inferred from Sequence Orthology more info
involved_in spermidine transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in xenobiotic transport	IEA Inferred from Electronic Annotation more info
involved_in xenobiotic transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in endomembrane system	IDA Inferred from Direct Assay more info	PubMed
located_in endomembrane system	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear outer membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: solute carrier family 22 member 3

Names: organic cation transporter 3; solute carrier family 22, member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011395.2 → NP_035525.1 solute carrier family 22 member 3

See identical proteins and their annotated locations for NP_035525.1

Status: PROVISIONAL

Source sequence(s)

AC087901, AC132106

Consensus CDS

CCDS28391.1

UniProtKB/Swiss-Prot

Q9R209, Q9WTW5

UniProtKB/TrEMBL

Q547K2, Q99JF0

Related

ENSMUSP00000024595.3, ENSMUST00000024595.4

Conserved Domains (2) summary

cd06174
Location:145 → 513

MFS; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of ...

TIGR00898
Location:12 → 523

2A0119; cation transport protein