Herc4 hect domain and RLD 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Herc4provided by MGI
Official Full Name: hect domain and RLD 4provided by MGI
Primary source: MGI:MGI:1914595
See related: Ensembl:ENSMUSG00000020064 AllianceGenome:MGI:1914595
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA1593; 1700056O17Rik; 4921531D01Rik; 9530080M15Rik
Summary: Predicted to enable ubiquitin protein ligase activity. Involved in spermatogenesis. Predicted to be located in cytosol and fibrillar center. Predicted to be active in cytoplasm. Orthologous to human HERC4 (HECT and RLD domain containing E3 ubiquitin protein ligase 4). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in testis adult (RPKM 13.5), bladder adult (RPKM 8.9) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B4; 10 32.56 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (63079122..63153660)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (63243751..63317881)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HERC4 plays an important role in Multiple myeloma progression.
Title: The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.
The E3 ubiquitin ligase Herc4, though ubiquitously expressed in all tissues, is most highly expressed in the testis, specifically during spermiogenesis
Title: Disruption of the ubiquitin ligase HERC4 causes defects in spermatozoon maturation and impaired fertility.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (2) 1 citation
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11Bhm20 (e-PCR), detects polymorphism
- UniSTS:141619

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

NoName (e-PCR)
- UniSTS:236367

NoName (e-PCR)
- UniSTS:236368

NoName (e-PCR)
- UniSTS:236369

AW553563 (e-PCR)
- UniSTS:208550

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info	PubMed
enables ubiquitin-protein transferase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of smoothened signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of smoothened signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	TAS Traceable Author Statement more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in fibrillar center	IEA Inferred from Electronic Annotation more info
located_in fibrillar center	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: probable E3 ubiquitin-protein ligase HERC4

Names: HECT domain and RCC1-like domain-containing protein 4; HECT-type E3 ubiquitin transferase HERC4

NP_001346413.1

EC 2.3.2.26

NP_080377.2

EC 2.3.2.26

NP_084390.1

EC 2.3.2.26

XP_006514049.1

EC 2.3.2.26

XP_006514052.1

EC 2.3.2.26

XP_006514053.1

EC 2.3.2.26

XP_030101093.1

EC 2.3.2.26

XP_030101094.1

EC 2.3.2.26

XP_036011855.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359484.2 → NP_001346413.1 probable E3 ubiquitin-protein ligase HERC4 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two consecutive in-frame exons in the central coding region and lacks an in-frame exon in the 3' coding region, compared to variant 1. It encodes a shorter isoform (3), compared to isoform 1.

Source sequence(s)

AC153516, AC155909

Conserved Domains (2) summary

cd00078
Location:580 → 926

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

cl27697
Location:2 → 344

RCC1; Regulator of chromosome condensation (RCC1) repeat
NM_001429479.1 → NP_001416408.1 probable E3 ubiquitin-protein ligase HERC4 isoform 4

Status: VALIDATED

Source sequence(s)

AC153516, AC155909
NM_001429480.1 → NP_001416409.1 probable E3 ubiquitin-protein ligase HERC4 isoform 5

Status: VALIDATED

Source sequence(s)

AC153516, AC155909
NM_001429481.1 → NP_001416410.1 probable E3 ubiquitin-protein ligase HERC4 isoform 6

Status: VALIDATED

Source sequence(s)

AC153516, AC155909
NM_026101.5 → NP_080377.2 probable E3 ubiquitin-protein ligase HERC4 isoform 2

See identical proteins and their annotated locations for NP_080377.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. It encodes a shorter isoform (2), compared to isoform 1.

Source sequence(s)

AC153516, AC155909

Consensus CDS

CCDS23897.1

UniProtKB/Swiss-Prot

Q6PAV2

Related

ENSMUSP00000020258.9, ENSMUST00000020258.10

Conserved Domains (3) summary

smart00119
Location:727 → 1046

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:701 → 1047

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00415
Location:208 → 257

RCC1; Regulator of chromosome condensation (RCC1) repeat
NM_030114.3 → NP_084390.1 probable E3 ubiquitin-protein ligase HERC4 isoform 1

See identical proteins and their annotated locations for NP_084390.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC153516, AC155909

Consensus CDS

CCDS88014.1

UniProtKB/Swiss-Prot

Q3UL34, Q3UPX2, Q6PAV2, Q810A0, Q811C9, Q8R315, Q9D797

Related

ENSMUSP00000151886.2, ENSMUST00000219577.2

Conserved Domains (3) summary

smart00119
Location:735 → 1054

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:709 → 1055

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00415
Location:208 → 257

RCC1; Regulator of chromosome condensation (RCC1) repeat

RNA

NR_190737.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC153516, AC155909
NR_190738.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC153516, AC155909

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

63079122..63153660

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006513989.4 → XP_006514052.1 probable E3 ubiquitin-protein ligase HERC4 isoform X3

Conserved Domains (2) summary

cd00078
Location:454 → 800

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00415
Location:5 → 53

RCC1; Regulator of chromosome condensation (RCC1) repeat
XM_006513986.4 → XP_006514049.1 probable E3 ubiquitin-protein ligase HERC4 isoform X2

Conserved Domains (2) summary

COG5184
Location:2 → 344

ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]

cd00078
Location:588 → 934

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_030245233.1 → XP_030101093.1 probable E3 ubiquitin-protein ligase HERC4 isoform X1

Conserved Domains (2) summary

COG5184
Location:12 → 268

ATS1; Alpha-tubulin suppressor and related RCC1 domain-containing proteins [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton]

cd00078
Location:639 → 985

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_030245234.1 → XP_030101094.1 probable E3 ubiquitin-protein ligase HERC4 isoform X3

Conserved Domains (2) summary

cd00078
Location:454 → 800

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00415
Location:5 → 53

RCC1; Regulator of chromosome condensation (RCC1) repeat
XM_036155962.1 → XP_036011855.1 probable E3 ubiquitin-protein ligase HERC4 isoform X5

Conserved Domains (1) summary

cd00078
Location:250 → 596

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_006513990.3 → XP_006514053.1 probable E3 ubiquitin-protein ligase HERC4 isoform X4

Conserved Domains (2) summary

cd00078
Location:446 → 792

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

pfam00415
Location:5 → 53

RCC1; Regulator of chromosome condensation (RCC1) repeat