Acsl4 acyl-CoA synthetase long-chain family member 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Acsl4provided by RGD
Official Full Name: acyl-CoA synthetase long-chain family member 4provided by RGD
Primary source: RGD:69401
See related: EnsemblRapid:ENSRNOG00000019180 AllianceGenome:RGD:69401
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Acs4; Facl4
Summary: Enables arachidonate-CoA ligase activity. Involved in several processes, including dendritic spine development; long-chain fatty-acyl-CoA biosynthetic process; and positive regulation of insulin secretion. Located in mitochondrial membrane; neuronal cell body; and peroxisome. Human ortholog(s) of this gene implicated in intellectual disability and non-syndromic X-linked intellectual disability 63. Orthologous to human ACSL4 (acyl-CoA synthetase long chain family member 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Adrenal (RPKM 1568.8), Liver (RPKM 1018.3) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq33

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (110739633..110803416, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (105942794..106006573, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (113596247..113660024, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ALKBH5 targets ACSL4 mRNA stability to modulate ferroptosis in hyperbilirubinemia-induced brain damage.
Title: ALKBH5 targets ACSL4 mRNA stability to modulate ferroptosis in hyperbilirubinemia-induced brain damage.
METTL3 promotes trophoblast ferroptosis in preeclampsia by stabilizing the ACSL4 m[6]A modification.
Title: METTL3 promotes trophoblast ferroptosis in preeclampsia by stabilizing the ACSL4 m(6)A modification.
YAP Facilitates NEDD4L-Mediated Ubiquitination and Degradation of ACSL4 to Alleviate Ferroptosis in Myocardial Ischemia-Reperfusion Injury.
Title: YAP Facilitates NEDD4L-Mediated Ubiquitination and Degradation of ACSL4 to Alleviate Ferroptosis in Myocardial Ischemia-Reperfusion Injury.
Acyl-CoA synthetase long chain family member 4 plays detrimental role in early brain injury after subarachnoid hemorrhage in rats by inducing ferroptosis.
Title: Acyl-CoA synthetase long chain family member 4 plays detrimental role in early brain injury after subarachnoid hemorrhage in rats by inducing ferroptosis.
Regulation and role of Acyl-CoA synthetase 4 in glial cells.
Title: Regulation and role of Acyl-CoA synthetase 4 in glial cells.
Upregulation of ACSL4 is responsible for the increase in triacylglycerol species containing long polyunsaturated fatty acids during activation of hepatic stellate cells.
Title: Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells.
Acsl isozymes play distinct roles in the control of AA remodeling in rat fibroblasts: Acsl4 acts as the first step of enzyme for AA remodeling following IL-1beta stimulation, and Acsl1 is involved in the maintenance of some AA-containing PC species.
Title: Role of long-chain acyl-coenzyme A synthetases in the regulation of arachidonic acid metabolism in interleukin 1β-stimulated rat fibroblasts.
Results suggest that long chain acyl-CoA synthetase Acsl4 activates epoxyeicosatrienoic acids (EETs) to form EET-CoAs that are incorporated into glycerophospholipids, thereby sequestering EETs
Title: Diminished acyl-CoA synthetase isoform 4 activity in INS 832/13 cells reduces cellular epoxyeicosatrienoic acid levels and results in impaired glucose-stimulated insulin secretion.
These results indicate that the ACS4 protein specifically expressed in brain plays an important role in arachidonate metabolism and neuronal differentiation in the brain.
Title: A novel role of brain-type ACS4 isotype in neuronal differentiation.
the involvement of SHP2 activity in the regulation of the expression of the fatty acid-metabolizing enzyme ACSL4
Title: Tyrosine phosphatase SHP2 regulates the expression of acyl-CoA synthetase ACSL4.

Submit: New GeneRIF Correction See all GeneRIFs (11)

General gene information

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Markers

BE096007 (e-PCR)
- UniSTS:243979

DXRat148 (e-PCR), detects polymorphism
- UniSTS:228420

Acsl4 (e-PCR), detects polymorphism
- UniSTS:502704

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables arachidonate-CoA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate-CoA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate-CoA ligase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate-CoA ligase activity	ISO Inferred from Sequence Orthology more info
enables long-chain fatty acid-CoA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables long-chain fatty acid-CoA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables long-chain fatty acid-CoA ligase activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables very long-chain fatty acid-CoA ligase activity	IEA Inferred from Electronic Annotation more info
enables very long-chain fatty acid-CoA ligase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in dendritic spine development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic process involved in female pregnancy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryonic process involved in female pregnancy	ISO Inferred from Sequence Orthology more info
involved_in fatty acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fatty acid transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in long-chain fatty acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in long-chain fatty acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in long-chain fatty acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in long-chain fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in long-chain fatty acid metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in long-chain fatty-acyl-CoA biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in long-chain fatty-acyl-CoA metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of prostaglandin secretion	ISO Inferred from Sequence Orthology more info
involved_in neuron differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to interleukin-15	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in triglyceride biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in lipid droplet	IBA Inferred from Biological aspect of Ancestor more info
located_in lipid droplet	IEA Inferred from Electronic Annotation more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in mitochondria-associated endoplasmic reticulum membrane contact site	IEA Inferred from Electronic Annotation more info
located_in mitochondria-associated endoplasmic reticulum membrane contact site	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal membrane	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: long-chain-fatty-acid--CoA ligase 4

Names: LACS 4; arachidonate--CoA ligase; fatty acid-Coenzyme A ligase, long chain 4; long-chain acyl-CoA synthetase 4

NP_446075.1

EC 6.2.1.15

EC 6.2.1.3

XP_006257376.1

EC 6.2.1.15

EC 6.2.1.3

XP_006257377.1

EC 6.2.1.15

EC 6.2.1.3

XP_006257378.1

EC 6.2.1.15

EC 6.2.1.3

XP_038955322.1

EC 6.2.1.15

EC 6.2.1.3

XP_038955323.1

EC 6.2.1.15

EC 6.2.1.3

XP_063135802.1

EC 6.2.1.15

EC 6.2.1.3

XP_063135803.1

EC 6.2.1.15

EC 6.2.1.3

XP_063135804.1

EC 6.2.1.15

EC 6.2.1.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001431649.1 → NP_001418578.1 long-chain-fatty-acid--CoA ligase 4 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

O35547
NM_001431650.1 → NP_001418579.1 long-chain-fatty-acid--CoA ligase 4 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

O35547
NM_001431651.1 → NP_001418580.1 long-chain-fatty-acid--CoA ligase 4 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A6KG71
NM_001431652.1 → NP_001418581.1 long-chain-fatty-acid--CoA ligase 4 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A6KG71
NM_001431653.1 → NP_001418582.1 long-chain-fatty-acid--CoA ligase 4 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A6KG71
NM_001431654.1 → NP_001418583.1 long-chain-fatty-acid--CoA ligase 4 isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A6KG71
NM_001431655.1 → NP_001418584.1 long-chain-fatty-acid--CoA ligase 4 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

O35547
NM_053623.2 → NP_446075.1 long-chain-fatty-acid--CoA ligase 4 isoform 2

See identical proteins and their annotated locations for NP_446075.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/TrEMBL

A0A8I6AVE1, A6KG71

Related

ENSRNOP00000026057.5, ENSRNOT00000026057.8

Conserved Domains (1) summary

cd17639
Location:80 → 655

LC_FACS_euk1; Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

110739633..110803416 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039099394.2 → XP_038955322.1 long-chain-fatty-acid--CoA ligase 4 isoform X1

Conserved Domains (1) summary

cd17639
Location:121 → 696

LC_FACS_euk1; Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins
XM_006257314.5 → XP_006257376.1 long-chain-fatty-acid--CoA ligase 4 isoform X1

Related

ENSRNOP00000103546.1, ENSRNOT00000130558.1

Conserved Domains (1) summary

cd17639
Location:121 → 696

LC_FACS_euk1; Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins
XM_006257316.4 → XP_006257378.1 long-chain-fatty-acid--CoA ligase 4 isoform X2

Conserved Domains (1) summary

cd17639
Location:80 → 655

LC_FACS_euk1; Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins
XM_063279734.1 → XP_063135804.1 long-chain-fatty-acid--CoA ligase 4 isoform X2
XM_006257315.5 → XP_006257377.1 long-chain-fatty-acid--CoA ligase 4 isoform X2

Conserved Domains (1) summary

cd17639
Location:80 → 655

LC_FACS_euk1; Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins
XM_063279733.1 → XP_063135803.1 long-chain-fatty-acid--CoA ligase 4 isoform X2
XM_063279732.1 → XP_063135802.1 long-chain-fatty-acid--CoA ligase 4 isoform X1
XM_039099395.2 → XP_038955323.1 long-chain-fatty-acid--CoA ligase 4 isoform X3

Conserved Domains (2) summary

PRK04813
Location:233 → 296

PRK04813; D-alanine--poly(phosphoribitol) ligase subunit DltA

cl17068
Location:121 → 479

AFD_class_I; Adenylate forming domain, Class I superfamily