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    waaG lipopolysaccharide glucosyltransferase I [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948149, updated on 3-Dec-2024

    Summary

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    Official Symbol
    waaG
    Official Full Name
    lipopolysaccharide glucosyltransferase I
    Primary source
    ECOCYC:EG11339
    Locus tag
    b3631
    See related
    ASAP:ABE-0011868
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3621; rfaG
    Summary
    Mutants have reduced OM vesiculation (McBroom, 2006). [More information is available at EcoGene: EG11339]. The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11339].
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    Genomic context

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    Sequence:
    NC_000913.3 (3805943..3807067, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-D-glucose:(glucosyl)LPS alpha-1,3-glucosyltransferase Neighboring gene UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase Neighboring gene lipopolysaccharide core biosynthesis protein WaaS Neighboring gene lipopolysaccharide core heptose (I) kinase Neighboring gene lipopolysaccharide core heptosyltransferase 3 Neighboring gene ncRNA Neighboring gene KDO transferase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Martinez-Fleites C, et al. Chem Biol, 2006 Nov. PMID 17113996
    2. Cloning genes for bacterial glycosyltransferases. Creeger ES, et al. Methods Enzymol, 1982. PMID 6212748
    3. Cloning of genes for bacterial glycosyltransferases. I. Selection of hybrid plasmids carrying genes for two glucosyltransferases. Creeger ES, et al. J Biol Chem, 1979 Feb 10. PMID 368061
    4. Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations. Pilipcinec E, et al. FEMS Microbiol Lett, 1994 Oct 15. PMID 7988890
    5. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. Yethon JA, et al. J Bacteriol, 2000 Oct. PMID 10986272, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. biofilm mutants with altered lipopolysaccharide synthesis (waaG) exhibited increased cell-cell interactions, bypassed the polar attachment step, and produced FFT spectra characteristic of aperiodic cell distribution.
      Title: Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables glycosyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hexosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lipopolysaccharide glucosyltransferase I activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables lipopolysaccharide glucosyltransferase I activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipopolysaccharide glucosyltransferase I activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in lipopolysaccharide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipopolysaccharide core region biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    lipopolysaccharide glucosyltransferase I

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3805943..3807067 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418088.1 lipopolysaccharide glucosyltransferase I [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418088.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5C9AFR4
      Conserved Domains (1) summary
      cd03801
      Location:3 → 368
      GT4_PimA-like; phosphatidyl-myo-inositol mannosyltransferase
    Nucleotide Protein
    Heading Accession and Version

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