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    thiI tRNA uridine 4-sulfurtransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945067, updated on 17-Dec-2024

    Summary

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    Official Symbol
    thiI
    Official Full Name
    tRNA uridine 4-sulfurtransferase
    Primary source
    ECOCYC:G6238
    Locus tag
    b0423
    See related
    ASAP:ABE-0001470
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0417; nuvA; yajK
    Summary
    The 4-thiouridine modification of tRNA acts as a near UV sensor because it makes tRNA near-UV labile, blocking translation, inducing the stringent response and stopping growth. [More information is available at EcoGene: EG13273]. ThiI is required for the synthesis of the thiazole moiety of thiamine and plays a role in the conversion of uridine to 4-thiouridine at position 8 in tRNA . [More information is available at EcoCyc: G6238].
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    Genomic context

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    Sequence:
    NC_000913.3 (441549..442997)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene geranyl diphosphate/farnesyl diphosphate synthase Neighboring gene exodeoxyribonuclease VII subunit XseB Neighboring gene protein/nucleic acid deglycase 3 Neighboring gene 2-dehydropantoate 2-reductase Neighboring gene nucleotide binding protein YajQ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Evidence that ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis, may be a sulfurtransferase that proceeds through a persulfide intermediate. Palenchar PM, et al. J Biol Chem, 2000 Mar 24. PMID 10722656
    2. Enzymes producing 4-thiouridine in Escherichia coli tRNA: approximate chromosomal locations of the genes and enzyme activities in a 4-thiouridine-deficient mutant. Lipsett MN. J Bacteriol, 1978 Sep. PMID 357427, Free PMC Article
    3. Isolation of single-site Escherichia coli mutants deficient in thiamine and 4-thiouridine syntheses: identification of a nuvC mutant. Ryals J, et al. J Bacteriol, 1982 Aug. PMID 6178725, Free PMC Article
    4. 4-Thiouridine triggers both growth delay induced by near-ultraviolet light and photoprotection. Thomas G, et al. Eur J Biochem, 1980 Dec. PMID 7007049
    5. Using genomic information to investigate the function of ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis. Mueller EG, et al. Protein Sci, 1999 Nov. PMID 10595545, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables CCA tRNA nucleotidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sulfur carrier activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfurtransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfurtransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA-uracil-4 sulfurtransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-cysteine catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in detection of UV NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in tRNA 4-thiouridine biosynthesis EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in tRNA 4-thiouridine biosynthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA 4-thiouridine biosynthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA thio-modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiamine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in thiamine biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in thiamine diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in thiazole biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in thiazole biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of L-cysteine desulfurase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of sulfurtransferase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA uridine 4-sulfurtransferase
    NP_414957.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      441549..442997
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414957.1 tRNA uridine 4-sulfurtransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414957.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1V2GJV7
      Conserved Domains (1) summary
      PRK01269
      Location:1 → 482
      PRK01269; tRNA s(4)U8 sulfurtransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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