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    glcB malate synthase G [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948857, updated on 3-Dec-2024

    Summary

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    Official Symbol
    glcB
    Official Full Name
    malate synthase G
    Primary source
    ECOCYC:EG20080
    Locus tag
    b2976
    See related
    ASAP:ABE-0009767
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2970; glc
    Summary
    There are two isozymes of malate synthase in Escherichia coli . [More information is available at EcoCyc: EG20080].
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    Genomic context

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    Sequence:
    NC_000913.3 (3121634..3123805, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative lipoprotein YghJ Neighboring gene glycolate/lactate:H(+) symporter GlcA Neighboring gene putative heme-binding protein GlcG Neighboring gene glycolate dehydrogenase, putative iron-sulfur subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Functional intermediate in the refolding pathway of a large and multidomain protein malate synthase G. Dahiya V, et al. Biochemistry, 2013 Jul 2. PMID 23718231
    2. [Purification and partial characterization of two malate synthases of Echerichia coli]. Falmagne P, et al. Eur J Biochem, 1973 Sep 3. PMID 4591144
    3. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 A resolution: mechanistic implications. Howard BR, et al. Biochemistry, 2000 Mar 21. PMID 10715138
    4. Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization. Anstrom DM, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2005 Dec 1. PMID 16511237, Free PMC Article
    5. Quantitative NMR studies of high molecular weight proteins: application to domain orientation and ligand binding in the 723 residue enzyme malate synthase G. Tugarinov V, et al. J Mol Biol, 2003 Apr 11. PMID 12662935

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This work has significant implications in the characterization of the refolding intermediates of multidomain proteins in general and MSG in particular
      Title: Functional intermediate in the refolding pathway of a large and multidomain protein malate synthase G.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme.
    3. present the solution global fold of the monomeric 723-residue (82-kDa) enzyme malate synthase G
      Title: Solution NMR-derived global fold of a monomeric 82-kDa enzyme.
    4. crystallographic analysis of replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G
      Title: Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables magnesium ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables malate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables malate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables malate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in glyoxylate catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within glyoxylate catabolic process IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in glyoxylate cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tricarboxylic acid cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    malate synthase G
    NP_417450.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3121634..3123805 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417450.1 malate synthase G [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417450.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7EBW3, A0A8T6PXC0
      Conserved Domains (1) summary
      PRK02999
      Location:1 → 723
      PRK02999; malate synthase G; Provisional
    Nucleotide Protein
    Heading Accession and Version

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