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    mnmC fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946800, updated on 17-Dec-2024

    Summary

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    Official Symbol
    mnmC
    Official Full Name
    fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase
    Primary source
    ECOCYC:G7199
    Locus tag
    b2324
    See related
    ASAP:ABE-0007681
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2318; trmC; yfcK
    Summary
    The tRNAs specific for glutamate, lysine, and possibly glutamine contain the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine (mnm5s2U) in position 34, the wobble position. [More information is available at EcoCyc: G7199].
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    Genomic context

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    Sequence:
    NC_000913.3 (2441764..2443770)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative flagella assembly protein Neighboring gene putative transporter YfcJ Neighboring gene 3-oxoacyl-[acyl carrier protein] synthase 1 Neighboring gene PF08891 family protein YfcL Neighboring gene EF-P-Lys34 hydroxylase Neighboring gene putative transporter YfcA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Identification of a novel 5-aminomethyl-2-thiouridine methyltransferase in tRNA modification. Cho G, et al. Nucleic Acids Res, 2023 Feb 28. PMID 36762482, Free PMC Article
    2. Assay of both activities of the bifunctional tRNA-modifying enzyme MnmC reveals a kinetic basis for selective full modification of cmnm5s2U to mnm5s2U. Pearson D, et al. Nucleic Acids Res, 2011 Jun. PMID 21306992, Free PMC Article
    3. Undermodification in the first position of the anticodon of supG-tRNA reduces translational efficiency. Hagervall TG, et al. Mol Gen Genet, 1984. PMID 6387394
    4. Genetic mapping and cloning of the gene (trmC) responsible for the synthesis of tRNA (mnm5s2U)methyltransferase in Escherichia coli K12. Hagervall TG, et al. Mol Gen Genet, 1984. PMID 6387395
    5. Escherichia coli mutants with defects in the biosynthesis of 5-methylaminomethyl-2-thio-uridine or 1-methylguanosine in their tRNA. Björk GR, et al. J Bacteriol, 1978 Feb. PMID 342495, Free PMC Article

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identification of a novel 5-aminomethyl-2-thiouridine methyltransferase in tRNA modification.
      Title: Identification of a novel 5-aminomethyl-2-thiouridine methyltransferase in tRNA modification.
    2. Investigation of the activities and the tRNA specificity of the two MnmC domains.
      Title: The output of the tRNA modification pathways controlled by the Escherichia coli MnmEG and MnmC enzymes depends on the growth conditions and the tRNA species.
    3. The structures of MnmC also reveal the detailed atomic interactions at the interdomain interface and provide spatial restraints relevant to the overall catalytic mechanism.
      Title: Structural basis for hypermodification of the wobble uridine in tRNA by bifunctional enzyme MnmC.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables FAD binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables S-adenosylmethionine-dependent methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables flavin adenine dinucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on the CH-NH group of donors IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tRNA methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble base modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA wobble uridine modification IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within tRNA wobble uridine modification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase
    NP_416827.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2441764..2443770
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416827.4 fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416827.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7I4W0
      Conserved Domains (1) summary
      PRK01747
      Location:2 → 666
      mnmC; bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC
    Nucleotide Protein
    Heading Accession and Version

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