Tbc1d4 TBC1 domain family, member 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Tbc1d4provided by RGD
Official Full Name: TBC1 domain family, member 4provided by RGD
Primary source: RGD:1561609
See related: EnsemblRapid:ENSRNOG00000009431 AllianceGenome:RGD:1561609
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1561609
Summary: Predicted to enable GTPase activator activity. Involved in cellular response to insulin stimulus and negative regulation of vesicle fusion. Located in vesicle. Human ortholog(s) of this gene implicated in acanthosis nigricans. Orthologous to human TBC1D4 (TBC1 domain family member 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 223.6), Muscle (RPKM 203.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 15q21

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	15	NC_086033.1 (84670756..84848876, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	15	NC_051350.1 (78256030..78434168, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	15	NC_005114.4 (85927978..86105829, complement)

Chromosome 15 - NC_086033.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of AS160-serine 704 is not essential for exercise-increase in insulin-stimulated glucose uptake by skeletal muscles from female or male rats.
Title: Phosphorylation of AS160-serine 704 is not essential for exercise-increase in insulin-stimulated glucose uptake by skeletal muscles from female or male rats.
AS160 expression, but not AS160 Serine-588, Threonine-642, and Serine-704 phosphorylation, is essential for elevated insulin-stimulated glucose uptake by skeletal muscle from female rats after acute exercise.
Title: AS160 expression, but not AS160 Serine-588, Threonine-642, and Serine-704 phosphorylation, is essential for elevated insulin-stimulated glucose uptake by skeletal muscle from female rats after acute exercise.
Akt substrate of 160 kDa is essential for the calorie restriction-induced increase in insulin-stimulated glucose uptake by skeletal muscle of female rats.
Title: Akt substrate of 160 kDa is essential for the calorie restriction-induced increase in insulin-stimulated glucose uptake by skeletal muscle of female rats.
Exercise-Induced Improvement in Insulin-Stimulated Glucose Uptake by Rat Skeletal Muscle Is Absent in Male AS160-Knockout Rats, Partially Restored by Muscle Expression of Phosphomutated AS160, and Fully Restored by Muscle Expression of Wild-Type AS160.
Title: Exercise-Induced Improvement in Insulin-Stimulated Glucose Uptake by Rat Skeletal Muscle Is Absent in Male AS160-Knockout Rats, Partially Restored by Muscle Expression of Phosphomutated AS160, and Fully Restored by Muscle Expression of Wild-Type AS160.
These results provided evidence that fiber type-specific pAS160 in insulin-resistant muscle may play a role in the previously reported fiber type-specific elevation in insulin-stimulated glucose uptake in some, but not all, fiber types.
Title: Fiber type-specific effects of acute exercise on insulin-stimulated AS160 phosphorylation in insulin-resistant rat skeletal muscle.
This unique AS160-KO rat model elucidated striking genotype-related modifications in glucoregulation.
Title: Whole body glucoregulation and tissue-specific glucose uptake in a novel Akt substrate of 160 kDa knockout rat model.
central injection of M617 mitigated insulin resistance of skeletal muscle by enhancing GLUT4 translocation from intracellular pools to plasma membranes via the activation of the Akt/AS160/GLUT4 signaling pathway.
Title: Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway.
Exposure of L6 skeletal muscle cells to an increased concentration of palmitate with subsequent modest knockdown of AS160 stimulates palmitic acid uptake by promoting an increase in the expression of FAT/CD36, FATP-1, 4.
Title: Challenging of AS160/TBC1D4 Alters Intracellular Lipid milieu in L6 Myotubes Incubated With Palmitate.
Results also indicate that decreased basal phosphorylation of AS160 may be involved in inactivity-induced insulin resistance.
Title: Immobilization rapidly induces muscle insulin resistance together with the activation of MAPKs (JNK and p38) and impairment of AS160 phosphorylation.
AS160 (TBC1D4) phosphorylation is essential for maintenance of insulin-stimulated glucose uptake and is rapidly reversed after insulin removal.
Title: Rapid reversal of insulin-stimulated AS160 phosphorylation in rat skeletal muscle after insulin exposure.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

TBC1D4 (e-PCR)
- UniSTS:505344

D15RAT71 (e-PCR)
- UniSTS:120205

AU028940 (e-PCR)
- UniSTS:111845

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to insulin stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vesicle fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle-mediated transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: TBC1 domain family member 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001427219.1 → NP_001414148.1 TBC1 domain family member 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000015

UniProtKB/TrEMBL

A0A8I6AT49

Related

ENSRNOP00000096754.1, ENSRNOT00000120033.2

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086033.1 Reference GRCr8

Range

84670756..84848876 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039093969.2 → XP_038949897.1 TBC1 domain family member 4 isoform X1

UniProtKB/TrEMBL

A0A8I6ABF1

Related

ENSRNOP00000089194.2, ENSRNOT00000109400.2

Conserved Domains (5) summary

smart00164
Location:873 → 1083

TBC; Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs

smart00462
Location:31 → 190

PTB; Phosphotyrosine-binding domain, phosphotyrosine-interaction (PI) domain

PTZ00440
Location:1087 → 1218

PTZ00440; reticulocyte binding protein 2-like protein; Provisional

cd01269
Location:193 → 446

PTB_TBC1D1_like; TBC1 domain family member 1 and related proteins Phosphotyrosine-binding (PTB) domain

pfam11830
Location:756 → 819

DUF3350; Domain of unknown function (DUF3350)
XM_039093970.2 → XP_038949898.1 TBC1 domain family member 4 isoform X2

UniProtKB/TrEMBL

A0A8I6ABF1, D3Z881

Related

ENSRNOP00000062670.4, ENSRNOT00000064942.6

Conserved Domains (5) summary

smart00164
Location:865 → 1075

TBC; Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs

smart00462
Location:31 → 190

PTB; Phosphotyrosine-binding domain, phosphotyrosine-interaction (PI) domain

PTZ00440
Location:1079 → 1210

PTZ00440; reticulocyte binding protein 2-like protein; Provisional

cd01269
Location:193 → 446

PTB_TBC1D1_like; TBC1 domain family member 1 and related proteins Phosphotyrosine-binding (PTB) domain

pfam11830
Location:748 → 811

DUF3350; Domain of unknown function (DUF3350)
XM_039093971.2 → XP_038949899.1 TBC1 domain family member 4 isoform X3

UniProtKB/TrEMBL

A6HU71

Conserved Domains (4) summary

smart00164
Location:739 → 949

TBC; Domain in Tre-2, BUB2p, and Cdc16p. Probable Rab-GAPs

PTZ00440
Location:953 → 1084

PTZ00440; reticulocyte binding protein 2-like protein; Provisional

cd01269
Location:12 → 265

PTB_TBC1D1_like; TBC1 domain family member 1 and related proteins Phosphotyrosine-binding (PTB) domain

pfam11830
Location:622 → 685

DUF3350; Domain of unknown function (DUF3350)