Net1 neuroepithelial cell transforming gene 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Net1provided by MGI
Official Full Name: neuroepithelial cell transforming gene 1provided by MGI
Primary source: MGI:MGI:1927138
See related: Ensembl:ENSMUSG00000021215 AllianceGenome:MGI:1927138
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Net1a; mNET1
Summary: Predicted to enable guanyl-nucleotide exchange factor activity. Acts upstream of or within myoblast migration. Predicted to be located in cytoplasm and nucleus. Is expressed in forelimb bud and submandibular gland primordium epithelium. Orthologous to human NET1 (neuroepithelial cell transforming 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in large intestine adult (RPKM 25.9), limb E14.5 (RPKM 18.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 A1; 13 2.17 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (3932018..3968220, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (3882018..3918220, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes.
Title: NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes.
Genetic deletion of the Rho GEF Net1 impairs mouse macrophage motility and actin cytoskeletal organization.
Title: Genetic deletion of the Rho GEF Net1 impairs mouse macrophage motility and actin cytoskeletal organization.
TNBS conjugated with NET-1 siRNA inhibited tumor growth.
Title: Evaluation of therapeutic effect of targeting nanobubbles conjugated with NET-1 siRNA by shear wave elastography: an in vivo study of hepatocellular carcinoma bearing mice model.
These data indicate that Net1 is required for tumor progression in the PyMT mouse model and suggest that Net1 may contribute to breast cancer progression in humans.
Title: Contributions of the RhoA guanine nucleotide exchange factor Net1 to polyoma middle T antigen-mediated mammary gland tumorigenesis and metastasis.
These data indicate that Net1A acetylation regulates its subcellular localization to impact on RhoA activity and actin cytoskeletal organization.
Title: Acetylation of the RhoA GEF Net1A controls its subcellular localization and activity.
Data identify for the first time a role for NET1 in vivo and indicate that NET1 expression is essential for the proliferation and differentiation of mammary epithelial cells in the developing mammary gland.
Title: The RhoGEF Net1 is required for normal mammary gland development.
PAK1 negatively regulates the activity of NET1
Title: PAK1 negatively regulates the activity of the Rho exchange factor NET1.
NET1 has roles in stimulating cytoskeletal reorganization and in transforming cells
Title: Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1.
regulation by nuclear sequestration
Title: The Rho exchange factor Net1 is regulated by nuclear sequestration.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI604373 (e-PCR)
- UniSTS:180492

BE199731 (e-PCR)
- UniSTS:208884

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hydrogen peroxide	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to ionizing radiation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to ionizing radiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within myoblast migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: neuroepithelial cell-transforming gene 1 protein

Names: Net1 homolog; guanine nucleotide regulatory protein (oncogene); rho guanine nucleotide exchange factor 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001047159.2 → NP_001040624.1 neuroepithelial cell-transforming gene 1 protein isoform 2

See identical proteins and their annotated locations for NP_001040624.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks part of the 5' coding region, uses an alternate 5'-terminal exon, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

Source sequence(s)

AK082128, AK139921, BB598013

Consensus CDS

CCDS36583.1

UniProtKB/TrEMBL

Q3USZ7

Related

ENSMUSP00000097529.5, ENSMUST00000099946.6

Conserved Domains (3) summary

smart00325
Location:124 → 300

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd13224
Location:314 → 448

PH_Net1; Neuroepithelial cell transforming 1 Pleckstrin homology (PH) domain

pfam00169
Location:333 → 447

PH; PH domain
NM_019671.3 → NP_062645.2 neuroepithelial cell-transforming gene 1 protein isoform 1

See identical proteins and their annotated locations for NP_062645.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC139323, AK082128, AK139921, CF167482

Consensus CDS

CCDS26216.1

UniProtKB/Swiss-Prot

Q8C4I0, Q9Z1L7, Q9Z206

Related

ENSMUSP00000089464.5, ENSMUST00000091853.12

Conserved Domains (3) summary

smart00325
Location:178 → 354

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd13224
Location:368 → 502

PH_Net1; Neuroepithelial cell transforming 1 Pleckstrin homology (PH) domain

pfam00169
Location:387 → 501

PH; PH domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

3932018..3968220 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030247332.2 → XP_030103192.1 neuroepithelial cell-transforming gene 1 protein isoform X1

Conserved Domains (2) summary

smart00325
Location:20 → 196

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd13224
Location:210 → 344

PH_Net1; Neuroepithelial cell transforming 1 Pleckstrin homology (PH) domain