Med16 mediator complex subunit 16 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Med16provided by MGI
Official Full Name: mediator complex subunit 16provided by MGI
Primary source: MGI:MGI:2158394
See related: Ensembl:ENSMUSG00000013833 AllianceGenome:MGI:2158394
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 95kDa; DRIP92; Thrap5; Trap95; A630083L04
Summary: Predicted to enable nuclear thyroid hormone receptor binding activity and transcription coactivator activity. Predicted to be involved in positive regulation of transcription initiation by RNA polymerase II. Predicted to act upstream of or within transcription by RNA polymerase II. Part of mediator complex. Is expressed in genitourinary system; limb; and tongue. Orthologous to human MED16 (mediator complex subunit 16). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 63.2), ovary adult (RPKM 52.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 C1; 10 39.72 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (79730543..79744757, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (79894706..79908938, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NRF2-dependent phosphorylation of the RNA polymerase II C-terminal domain was absent in Med16-deficient cells, suggesting that MED16 serves as a conduit to transmit NRF2-activating signals to RNA polymerase II.
Title: The Mediator Subunit MED16 Transduces NRF2-Activating Signals into Antioxidant Gene Expression.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI503502 (e-PCR)
- UniSTS:180070

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables nuclear thyroid hormone receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear thyroid hormone receptor binding	ISS Inferred from Sequence or Structural Similarity more info
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transcription coactivator activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in RNA polymerase II preinitiation complex assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription elongation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription initiation by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription initiation by RNA polymerase II	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within transcription by RNA polymerase II	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
part_of core mediator complex	ISO Inferred from Sequence Orthology more info
part_of mediator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mediator complex	IDA Inferred from Direct Assay more info	PubMed
part_of mediator complex	ISO Inferred from Sequence Orthology more info
part_of mediator complex	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mediator of RNA polymerase II transcription subunit 16

Names: thyroid hormone receptor associated protein 5; thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; vitamin D3 receptor-interacting protein complex 92 kDa component

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163276.2 → NP_001156748.2 mediator of RNA polymerase II transcription subunit 16 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a different splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter by 1 aa when it is compared to isoform 1.

Source sequence(s)

AC151846

Consensus CDS

CCDS48623.1

UniProtKB/Swiss-Prot

E9QM81, Q6PGF3

Related

ENSMUSP00000101017.4, ENSMUST00000105378.9
NM_001359629.2 → NP_001346558.2 mediator of RNA polymerase II transcription subunit 16 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform 3), has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC151846
NM_001403459.1 → NP_001390388.1 mediator of RNA polymerase II transcription subunit 16 isoform 1

Status: VALIDATED

Source sequence(s)

AC151846

UniProtKB/Swiss-Prot

E9QM81, Q6PGF3
NM_198107.3 → NP_932775.3 mediator of RNA polymerase II transcription subunit 16 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest protein (isoform 1).

Source sequence(s)

AC151846

Consensus CDS

CCDS23996.2

UniProtKB/Swiss-Prot

E9QM81, Q6PGF3

Related

ENSMUSP00000129375.3, ENSMUST00000165684.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

79730543..79744757 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155735.1 → XP_036011628.1 mediator of RNA polymerase II transcription subunit 16 isoform X1

UniProtKB/TrEMBL

E9QP84

Conserved Domains (3) summary

smart00320
Location:59 → 98

WD40; WD40 repeats

sd00039
Location:26 → 68

7WD40; WD40 repeat [structural motif]

pfam11635
Location:108 → 822

Med16; Mediator complex subunit 16