sep-1 Separin homolog sep-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: sep-1
Official Full Name: Separin homolog sep-1
Primary source: WormBase:WBGene00004775
Locus tag: CELE_Y47G6A.12
See related: AllianceGenome:WB:WBGene00004775
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable cysteine-type endopeptidase activity. Involved in several processes, including cortical granule exocytosis; nuclear division; and polarity specification of anterior/posterior axis. Located in several cellular components, including cortical granule; microtubule cytoskeleton; and nucleus. Orthologous to human ESPL1 (extra spindle pole bodies like 1, separase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See sep-1 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 10

Sequence:: Chromosome: I; NC_003279.8 (3433158..3438543, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data indicate that protein phosphatase 5 is an important regulator of separase.
Title: Genetic Identification of Separase Regulators in Caenorhabditis elegans.
These findings suggest that separase cleaves an unknown substrate to promote the exocytosis of RAB-11 vesicles and paves the way for biochemical identification of substrates.
Title: Protease dead separase inhibits chromosome segregation and RAB-11 vesicle trafficking.
single-particle cryo-electron microscopy (cryo-EM) was used to determine a near-atomic-resolution structure of the Caenorhabditis elegans separase-securin complex.
Title: Cryo-EM structure of a metazoan separase-securin complex at near-atomic resolution.
Examination of the N-terminal tail sequence of CPAR-1 revealed a putative separase cleavage site and mutation of the signature residues in this site eliminated the cleavage reaction.
Title: Separase Cleaves the N-Tail of the CENP-A Related Protein CPAR-1 at the Meiosis I Metaphase-Anaphase Transition in C. elegans.
separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody
Title: A role for separase in the regulation of RAB-11-positive vesicles at the cleavage furrow and midbody.
SEP-1 has two separable yet coordinated functions: to regulate cortical granule exocytosis and to mediate chromosome separation
Title: Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y47G6A_246.b (e-PCR)
- UniSTS:322955

Y47G6A_246.c (e-PCR)
- UniSTS:324606

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type endopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in cortical granule exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in eggshell formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic chromosome separation	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic chromosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in multicellular organismal reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in nuclear division	IEA Inferred from Electronic Annotation more info
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polarity specification of anterior/posterior axis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of centriole-centriole cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
located_in cleavage furrow	IEA Inferred from Electronic Annotation more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cortical granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in meiotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in metaphase plate	IDA Inferred from Direct Assay more info	PubMed
located_in midbody	IDA Inferred from Direct Assay more info	PubMed
located_in midbody	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Separin homolog sep-1

NP_491160.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

3433158..3438543 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_058759.7 → NP_491160.1 Separin homolog sep-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_491160.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5ED39

Conserved Domains (1) summary

pfam03568
Location:727 → 1081

Peptidase_C50; Peptidase family C50