FUN12 translation initiation factor eIF5B [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FUN12
Official Full Name: translation initiation factor eIF5B
Primary source: SGD:S000000033
Locus tag: YAL035W
See related: AllianceGenome:SGD:S000000033; FungiDB:YAL035W; VEuPathDB:YAL035W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: yIF2
Summary: Enables several functions, including GTP binding activity; GTPase activity; and nucleic acid binding activity. Involved in formation of cytoplasmic translation initiation complex; regulation of translational initiation; and ribosome biogenesis. Located in cytoplasmic stress granule; cytosol; and mitochondrion. Part of cytosolic small ribosomal subunit and eukaryotic 48S preinitiation complex. Orthologous to human EIF5B (eukaryotic translation initiation factor 5B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See FUN12 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (76427..79435)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex.
Title: Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex.
Cryo-EM structures indicate eukaryotic initiation factor eIF3 as a molecular embracer of the 40S subunit, and the binding of eIF1 and eIF5 further fortified the compact architecture of eIF3.
Title: Binding of eIF3 in complex with eIF5 and eIF1 to the 40S ribosomal subunit is accompanied by dramatic structural changes.
The structures of two isolated forms of yeast eIF5B and of the eIF5B-eIF1A complex (eIF1A and eIF5B do not contain the respective N-terminal domains) are reported.
Title: X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A.
propose that recruitment of 60S subunits promotes GTP hydrolysis by Fun12, leading to structural rearrangements within the pre-40S particle that bring Nob1 and the pre-rRNA cleavage site together
Title: Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits.
helix H12 is not critical for eIF5B binding to the ribosome or for GTP hydrolysis with purified ribosomes; however, the length and rigidity of helix H12 are important for formation of stable 80S ribosomal complexes.
Title: Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability.
A mutation in helix h5 of the 18S rRNA in the 40S ribosomal subunit and intragenic mutations in domain II of eIF5B suppress the toxic effects associated with expression of the eIF5B-H480I GTPase-deficient mutant in yeast.
Title: rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables membrane scission GTPase motor activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables small ribosomal subunit rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cytosolic ribosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formation of cytoplasmic translation initiation complex	IDA Inferred from Direct Assay more info	PubMed
involved_in formation of cytoplasmic translation initiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
part_of cytosolic small ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic 48S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF5B

NP_009365.1

EC 3.6.5.3

Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001133.9 Reference assembly

Range

76427..79435

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178180.1 → NP_009365.1 TPA: translation initiation factor eIF5B [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009365.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPI2, P39730

UniProtKB/TrEMBL

B3LUX0, B5VDI4, N1P922

Conserved Domains (1) summary

cl30717
Location:400 → 964

InfB; Translation initiation factor IF-2, a GTPase [Translation, ribosomal structure and biogenesis]