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    NTG1 bifunctional N-glycosylase/AP lyase NTG1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851218, updated on 9-Dec-2024

    Summary

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    Official Symbol
    NTG1
    Official Full Name
    bifunctional N-glycosylase/AP lyase NTG1
    Primary source
    SGD:S000000013
    Locus tag
    YAL015C
    See related
    AllianceGenome:SGD:S000000013; FungiDB:YAL015C; VEuPathDB:YAL015C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    FUN33; ogg2; SCR1
    Summary
    Enables DNA-(apurinic or apyrimidinic site) endonuclease activity; oxidized purine nucleobase lesion DNA N-glycosylase activity; and oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity. Involved in base-excision repair, AP site formation; cellular response to oxidative stress; and positive regulation of mitochondrial DNA replication. Located in mitochondrion and nucleus. Human ortholog(s) of this gene implicated in familial adenomatous polyposis 3. Orthologous to human NTHL1 (nth like DNA glycosylase 1). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See NTG1 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    1
    Sequence:
    Chromosome: I; NC_001133.9 (126903..128102, complement)

    Chromosome I - NC_001133.9Genomic Context describing neighboring genes Neighboring gene serine/threonine protein kinase PSK1 Neighboring gene protein phosphatase 2A structural subunit TPD3 Neighboring gene syntaxin Neighboring gene Rpd3L histone deacetylase complex subunit DEP1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001133.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Regulation of base excision repair: Ntg1 nuclear and mitochondrial dynamic localization in response to genotoxic stress. Swartzlander DB, et al. Nucleic Acids Res, 2010 Jul. PMID 20194111, Free PMC Article
    2. Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli. Eide L, et al. Proc Natl Acad Sci U S A, 1996 Oct 1. PMID 8855249, Free PMC Article
    3. Inactivation of Saccharomyces cerevisiae OGG1 DNA repair gene leads to an increased frequency of mitochondrial mutants. Singh KK, et al. Nucleic Acids Res, 2001 Mar 15. PMID 11239005, Free PMC Article
    4. Efficient removal of formamidopyrimidines by 8-oxoguanine glycosylases. Krishnamurthy N, et al. Biochemistry, 2008 Jan 22. PMID 18154319, Free PMC Article
    5. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7. Yoshitani A, et al. Biochem Biophys Res Commun, 2008 Jan 4. PMID 17988640

    See all (92) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress.
      Title: Identification of SUMO modification sites in the base excision repair protein, Ntg1.
    2. the elements necessary for nuclear and mitochondrial localization of Ntg1 were identified, including a bipartite classical nuclear localization signal, a mitochondrial matrix targeting sequence and the classical nuclear protein import machinery.
      Title: Regulation of base excision repair: Ntg1 nuclear and mitochondrial dynamic localization in response to genotoxic stress.
    3. The intracellular localization of the Saccharomyces cerevisiae N-glycosylase/AP lyases, Ntg1 and Ntg2, in response to nuclear and mitochondrial oxidative stress, was determined.
      Title: Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress.
    4. The superb activity of the 8-oxoguanine glycosylases Fpg, hOGG1, and Ntg1 toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo.
      Title: Efficient removal of formamidopyrimidines by 8-oxoguanine glycosylases.
    5. loss of Ntg1p suppresses mitochondrial point mutation rates, frameshifts and recombination rates
      Title: Ntg1p, the base excision repair protein, generates mutagenic intermediates in yeast mitochondrial DNA.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA N-glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables lyase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidized base lesion DNA N-glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidized purine nucleobase lesion DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in base-excision repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in base-excision repair, AP site formation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in base-excision repair, AP site formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in base-excision repair, AP site formation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to oxidative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nucleotide-excision repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in positive regulation of mitochondrial DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    bifunctional N-glycosylase/AP lyase NTG1
    NP_009387.1
    • DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001133.9 Reference assembly

      Range
      126903..128102 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178160.1 → NP_009387.1  TPA: bifunctional N-glycosylase/AP lyase NTG1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009387.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VPK3, P31378
      UniProtKB/TrEMBL
      A7A0F4, B5VDK8, C7GPM0, C8Z3L5, G2W8L7, N1P7K4
      Conserved Domains (1) summary
      COG0177
      Location:140 → 318
      Nth; Endonuclease III [Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P31378.1

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