Mkrn2 makorin, ring finger protein, 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mkrn2provided by MGI
Official Full Name: makorin, ring finger protein, 2provided by MGI
Primary source: MGI:MGI:1914277
See related: Ensembl:ENSMUSG00000000439 AllianceGenome:MGI:1914277
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2610002L04Rik
Summary: Enables ubiquitin protein ligase activity. Involved in negative regulation of inflammatory response to antigenic stimulus; negative regulation of non-canonical NF-kappaB signal transduction; and ubiquitin-dependent protein catabolic process. Located in cytoplasm and nucleus. Is expressed in several structures, including central nervous system; early conceptus; and genitourinary system. Orthologous to human MKRN2 (makorin ring finger protein 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in testis adult (RPKM 33.7), adrenal adult (RPKM 19.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 E3; 6 53.61 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (115578844..115598647)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (115601883..115621683)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MKRN2 knockout causes male infertility through decreasing STAT1, SIX4, and TNC expression.
Title: MKRN2 knockout causes male infertility through decreasing STAT1, SIX4, and TNC expression.
Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis.
Title: Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis.
These results delineate a novel role of MKRN2 in negatively regulating NF-kappaB-mediated inflammatory responses, cooperatively with PDLIM2.
Title: MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses.
The authors developed an Mrkn2 knockout mouse model to study the role of this gene, and found that deletion of Mkrn2 in mice led to male infertility and expression of Odf2, a vital protein in spermatogenesis, was significantly decreased.
Title: Deficiency of Mkrn2 causes abnormal spermiogenesis and spermiation, and impairs male fertility.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH127069 (e-PCR)
- UniSTS:208014

AA990557 (e-PCR)
- UniSTS:160660

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response to antigenic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of non-canonical NF-kappaB signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase makorin-2

Names: Makorin RING zinc-finger protein 2; RING-type E3 ubiquitin transferase makorin-2; probable E3 ubiquitin-protein ligase makorin-2

NP_075779.2

EC 2.3.2.27

XP_011239733.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_023290.3 → NP_075779.2 E3 ubiquitin-protein ligase makorin-2

See identical proteins and their annotated locations for NP_075779.2

Status: VALIDATED

Source sequence(s)

AC160032

Consensus CDS

CCDS39600.1

UniProtKB/Swiss-Prot

Q6GTY9, Q9D0L9, Q9ERV1

UniProtKB/TrEMBL

Q9D776

Related

ENSMUSP00000000449.8, ENSMUST00000000449.9

Conserved Domains (3) summary

smart00356
Location:35 → 55

ZnF_C3H1; zinc finger

PHA03096
Location:178 → 327

PHA03096; p28-like protein; Provisional

cd00162
Location:238 → 292

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

115578844..115598647

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011241431.3 → XP_011239733.1 E3 ubiquitin-protein ligase makorin-2 isoform X1

Conserved Domains (2) summary

COG5252
Location:102 → 175

COG5252; Uncharacterized conserved protein, contains CCCH-type Zn-finger protein [General function prediction only]

cd16731
Location:37 → 94

RING-HC_MKRN2; RING finger, HC subclass, found in makorin-2 (MKRN2) and similar proteins