ECM16 ATP-dependent RNA helicase ECM16 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ECM16
Official Full Name: ATP-dependent RNA helicase ECM16
Primary source: SGD:S000004735
Locus tag: YMR128W
See related: AllianceGenome:SGD:S000004735; FungiDB:YMR128W; VEuPathDB:YMR128W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DHR1
Summary: Enables RNA helicase activity. Involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA). Located in nucleolus. Part of small-subunit processome. Used to study microcephaly. Human ortholog(s) of this gene implicated in 46,XY sex reversal. Orthologous to human DHX37 (DEAH-box helicase 37). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ECM16 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (523696..527499)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In vitro characterization of Dhr1 from Saccharomyces cerevisiae.
Title: In vitro characterization of Dhr1 from Saccharomyces cerevisiae.
The presence of a carboxyl-terminal domain is essential for Dhr1 protein, helicase (Dhr1) function in ribosome biogenesis in vivo.
Title: The DEAH-box RNA helicase Dhr1 contains a remarkable carboxyl terminal domain essential for small ribosomal subunit biogenesis.
Utp14 is bifunctional; together with Bud23, it is needed for stable interaction of Dhr1 with the preribosome, and Utp14 activates Dhr1 to dislodge U3.
Title: Utp14 Recruits and Activates the RNA Helicase Dhr1 To Undock U3 snoRNA from the Preribosome.
Dhr1 dissociates U3 snoRNA from the pre-rRNA to promote formation of the central pseudoknot.
Title: The DEAH-box helicase Dhr1 dissociates U3 from the pre-rRNA to promote formation of the central pseudoknot.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on RNA	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in ribonucleoprotein complex biogenesis	IEA Inferred from Electronic Annotation more info
involved_in ribosome biogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of 90S preribosome	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleolus	HDA more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
part_of small-subunit processome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ATP-dependent RNA helicase ECM16

NP_013847.1

EC 3.6.4.13

Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; functions with methyltransferase Bud23p; predominantly nucleolar in distribution; required for 18S rRNA synthesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

523696..527499

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182629.1 → NP_013847.1 TPA: ATP-dependent RNA helicase ECM16 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013847.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZV1, Q04217

UniProtKB/TrEMBL

B3LM19, C8ZEY3

Conserved Domains (1) summary

COG1643
Location:360 → 1267

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]