ESP1 separase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ESP1
Official Full Name: separase
Primary source: SGD:S000003330
Locus tag: YGR098C
See related: AllianceGenome:SGD:S000003330; FungiDB:YGR098C; VEuPathDB:YGR098C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cysteine-type endopeptidase activity. Involved in several processes, including negative regulation of protein localization to nucleolus; nuclear division; and regulation of cell cycle process. Located in cytoplasm; nucleus; and spindle. Orthologous to human ESPL1 (extra spindle pole bodies like 1, separase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ESP1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (682566..687458, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
Title: Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.
These data revealed the genes ESP1 and MET22 as two, so far unknown, genetic determinants of resuming proliferation upon exposure to acetic acid.
Title: Identification of novel genes involved in acetic acid tolerance of Saccharomyces cerevisiae using pooled-segregant RNA sequencing.
Esp1 is phosphorylated by Cdk1 and dephosphorylated by Cdc55.Esp1 role in the regulation pericentric cohesin and anaphase onset.
Title: Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset.
crystal structures at up to 2.6 A resolution of the yeast Saccharomyces cerevisiae separase-securin complex
Title: Molecular mechanism for the regulation of yeast separase by securin.
We propose that Esp1 serves two roles to mediate Ty1 transposition - one to remove cohesin and the second to target Ty1-IN to chromatin
Title: A role for the budding yeast separase, Esp1, in Ty1 element retrotransposition.
Separase acts directly on Scc1 and also indirectly, through inhibition of PP2A(Cdc55), to stimulate cohesin cleavage, providing a feedforward loop that may contribute to a robust and timely anaphase.
Title: Separase biosensor reveals that cohesin cleavage timing depends on phosphatase PP2A(Cdc55) regulation.
phosphorylation either of separase or cohesin may be necessary for Rec8 cleavage
Title: Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis.
Esp1 is not essential for mitotic exit and contributes to the timing of mitotic exit mainly through promoting cohesin cleavage.
Title: Mitotic exit in the absence of separase activity.
Sgo1 can inhibit separase activity independently of securin.
Title: Shugoshin prevents cohesin cleavage by PP2A(Cdc55)-dependent inhibition of separase.
The activated Esp1 acts as caspase-like protease for the cleavage of Mcd1, which enhances the cell death via its translocation from nucleus to mitochondria.
Title: Cleavage of Mcd1 by caspase-like protease Esp1 promotes apoptosis in budding yeast.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in meiosis II	IDA Inferred from Direct Assay more info	PubMed
involved_in meiosis II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic chromosome separation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein localization to nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in nuclear division	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of exit from mitosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of mitotic spindle elongation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitotic spindle	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitotic spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: separase

NP_011612.3

EC 3.4.22.49

Separase/separin, a caspase-like cysteine protease; cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit, resulting in the dissociation of cohesin from chromatin and sister chromatid separation; cleaves meiotic-cohesin subunit Rec8p along chromosome arms in meiosis I and at centromeric sites during meiosis II; inhibits PP2A-Cdc55p to promote mitotic exit; inhibited by Pds1p (securin); relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

682566..687458 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181227.3 → NP_011612.3 TPA: separase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011612.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUN0, Q03018

UniProtKB/TrEMBL

B3LIC8, C7GWW5

Conserved Domains (1) summary

COG5155
Location:1 → 1630

ESP1; Separase, a protease involved in sister chromatid separation [Cell division and chromosome partitioning / Posttranslational modification, protein turnover, chaperones]