Keap1 kelch-like ECH-associated protein 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Keap1provided by MGI
Official Full Name: kelch-like ECH-associated protein 1provided by MGI
Primary source: MGI:MGI:1858732
See related: Ensembl:ENSMUSG00000003308 AllianceGenome:MGI:1858732
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: INRF2; mKIAA0132
Summary: Enables disordered domain specific binding activity and identical protein binding activity. Involved in several processes, including cellular response to oxidative stress; protein ubiquitination; and ubiquitin-dependent protein catabolic process. Acts upstream of or within several processes, including cellular response to interleukin-4; in utero embryonic development; and regulation of epidermal cell differentiation. Located in actin filament; endoplasmic reticulum; and inclusion body. Part of Cul3-RING ubiquitin ligase complex. Is expressed in eye and limb mesenchyme. Human ortholog(s) of this gene implicated in gallbladder cancer. Orthologous to human KEAP1 (kelch like ECH associated protein 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in ovary adult (RPKM 41.2), adrenal adult (RPKM 36.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A3; 9 7.74 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (21141026..21150628, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (21229730..21239332, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Homocysteine-potentiated Kelch-like ECH-associated protein 1 promotes senescence of neuroblastoma 2a cells via inhibiting ubiquitination of beta-catenin.
Title: Homocysteine-potentiated Kelch-like ECH-associated protein 1 promotes senescence of neuroblastoma 2a cells via inhibiting ubiquitination of β-catenin.
Dual Deletion of Keap1 and Rbpjkappa Genes in Liver Leads to Hepatomegaly and Hypercholesterolemia.
Title: Dual Deletion of Keap1 and Rbpjκ Genes in Liver Leads to Hepatomegaly and Hypercholesterolemia.
Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2.
Title: Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2.
GSTP alleviates acute lung injury by S-glutathionylation of KEAP1 and subsequent activation of NRF2 pathway.
Title: GSTP alleviates acute lung injury by S-glutathionylation of KEAP1 and subsequent activation of NRF2 pathway.
KEAP1-Mutant Lung Cancers Weaken Anti-Tumor Immunity and Promote an M2-like Macrophage Phenotype.
Title: KEAP1-Mutant Lung Cancers Weaken Anti-Tumor Immunity and Promote an M2-like Macrophage Phenotype.
Sestrin2 reduces ferroptosis via the Keap1/Nrf2 signaling pathway after intestinal ischemia-reperfusion.
Title: Sestrin2 reduces ferroptosis via the Keap1/Nrf2 signaling pathway after intestinal ischemia-reperfusion.
Effect of arsenic and copper in kidney of mice: Crosstalk between Nrf2/ Keap1 pathway in apoptosis and pyroptosis.
Title: Effect of arsenic and copper in kidney of mice: Crosstalk between Nrf2/ Keap1 pathway in apoptosis and pyroptosis.
FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice.
Title: FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice.
Nrf2 activation improves experimental rheumatoid arthritis.
Title: Nrf2 activation improves experimental rheumatoid arthritis.
Dynamic change and preventive role of stress response via Keap1-Nrf2 during renal crystal formation.
Title: Dynamic change and preventive role of stress response via Keap1-Nrf2 during renal crystal formation.

Submit: New GeneRIF Correction See all GeneRIFs (154)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (13) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

RH138725 (e-PCR)
- UniSTS:220812

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables disordered domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription regulator inhibitor activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like ligase-substrate adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-like ligase-substrate adaptor activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like ligase-substrate adaptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to carbohydrate stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to interleukin-4	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of response to oxidative stress	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of autophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of epidermal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of Cul3-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul3-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cul3-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of Cul3-RING ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in adherens junction	ISO Inferred from Sequence Orthology more info
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in inclusion body	IDA Inferred from Direct Assay more info	PubMed
located_in inclusion body	ISO Inferred from Sequence Orthology more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: kelch-like ECH-associated protein 1

Names: NRF2 cytosolic inhibitor; cytosolic inhibitor of Nrf2; ring canal protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110305.1 → NP_001103775.1 kelch-like ECH-associated protein 1

See identical proteins and their annotated locations for NP_001103775.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein.

Source sequence(s)

AC163637, AK150485, AK159858, BY586021

Consensus CDS

CCDS22897.1

UniProtKB/Swiss-Prot

Q3U243, Q3U8N7, Q547S3, Q6ZQI6, Q8BQY3, Q9Z2X8

Related

ENSMUSP00000062467.5, ENSMUST00000049567.10

Conserved Domains (5) summary

smart00612
Location:471 → 517

Kelch; Kelch domain

PHA03098
Location:95 → 596

PHA03098; kelch-like protein; Provisional

sd00038
Location:460 → 503

Kelch; KELCH repeat [structural motif]

pfam00651
Location:67 → 176

BTB; BTB/POZ domain

pfam07707
Location:184 → 286

BACK; BTB And C-terminal Kelch
NM_001110306.1 → NP_001103776.1 kelch-like ECH-associated protein 1

See identical proteins and their annotated locations for NP_001103776.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein.

Source sequence(s)

AC163637, AK004738, BY586021, CJ179527

Consensus CDS

CCDS22897.1

UniProtKB/Swiss-Prot

Q3U243, Q3U8N7, Q547S3, Q6ZQI6, Q8BQY3, Q9Z2X8

Related

ENSMUSP00000141840.2, ENSMUST00000193982.2

Conserved Domains (5) summary

smart00612
Location:471 → 517

Kelch; Kelch domain

PHA03098
Location:95 → 596

PHA03098; kelch-like protein; Provisional

sd00038
Location:460 → 503

Kelch; KELCH repeat [structural motif]

pfam00651
Location:67 → 176

BTB; BTB/POZ domain

pfam07707
Location:184 → 286

BACK; BTB And C-terminal Kelch
NM_001110307.1 → NP_001103777.1 kelch-like ECH-associated protein 1

See identical proteins and their annotated locations for NP_001103777.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3, and 4 all encode the same protein.

Source sequence(s)

AK004738, BY586021

Consensus CDS

CCDS22897.1

UniProtKB/Swiss-Prot

Q3U243, Q3U8N7, Q547S3, Q6ZQI6, Q8BQY3, Q9Z2X8

Related

ENSMUSP00000141807.2, ENSMUST00000194542.6

Conserved Domains (5) summary

smart00612
Location:471 → 517

Kelch; Kelch domain

PHA03098
Location:95 → 596

PHA03098; kelch-like protein; Provisional

sd00038
Location:460 → 503

Kelch; KELCH repeat [structural motif]

pfam00651
Location:67 → 176

BTB; BTB/POZ domain

pfam07707
Location:184 → 286

BACK; BTB And C-terminal Kelch
NM_016679.4 → NP_057888.1 kelch-like ECH-associated protein 1

See identical proteins and their annotated locations for NP_057888.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2, 3, and 4 all encode the same protein.

Source sequence(s)

AC163637, AK076234, BY586021

Consensus CDS

CCDS22897.1

UniProtKB/Swiss-Prot

Q3U243, Q3U8N7, Q547S3, Q6ZQI6, Q8BQY3, Q9Z2X8

Related

ENSMUSP00000131029.2, ENSMUST00000164812.8

Conserved Domains (5) summary

smart00612
Location:471 → 517

Kelch; Kelch domain

PHA03098
Location:95 → 596

PHA03098; kelch-like protein; Provisional

sd00038
Location:460 → 503

Kelch; KELCH repeat [structural motif]

pfam00651
Location:67 → 176

BTB; BTB/POZ domain

pfam07707
Location:184 → 286

BACK; BTB And C-terminal Kelch