Pkn1 protein kinase N1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pkn1provided by MGI
Official Full Name: protein kinase N1provided by MGI
Primary source: MGI:MGI:108022
See related: Ensembl:ENSMUSG00000057672 AllianceGenome:MGI:108022
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DBK; Pkn; PAK1; PRK1; Stk3; Prkcl1; F730027O18Rik
Summary: Enables protein kinase activity. Acts upstream of or within several processes, including negative regulation of B cell proliferation; regulation of germinal center formation; and spleen development. Located in cytoplasmic vesicle. Is expressed in several structures, including brown fat; genitourinary system; gut; nervous system; and vascular system. Orthologous to human PKN1 (protein kinase N1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 55.4), spleen adult (RPKM 49.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C2; 8 40.22 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (84393162..84425819, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (83666533..83699181, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion.
Title: Protein kinase N promotes cardiac fibrosis in heart failure by fibroblast-to-myofibroblast conversion.
PKN1 Exerts Neurodegenerative Effects in an In Vitro Model of Cerebellar Hypoxic-Ischemic Encephalopathy via Inhibition of AKT/GSK3beta Signaling.
Title: PKN1 Exerts Neurodegenerative Effects in an In Vitro Model of Cerebellar Hypoxic-Ischemic Encephalopathy via Inhibition of AKT/GSK3β Signaling.
Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
Title: Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
PKN1 promotes synapse maturation by inhibiting mGluR-dependent silencing through neuronal glutamate transporter activation.
Title: PKN1 promotes synapse maturation by inhibiting mGluR-dependent silencing through neuronal glutamate transporter activation.
Novel roles of PRK1 and PRK2 in cilia and cancer biology.
Title: Novel roles of PRK1 and PRK2 in cilia and cancer biology.
PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes.
Title: PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes.
PKN1 controls the aggregation, spheroid formation, and viability of mouse embryonic fibroblasts in suspension culture.
Title: PKN1 controls the aggregation, spheroid formation, and viability of mouse embryonic fibroblasts in suspension culture.
PKN1 and PKN2 activation causes cardiac dysfunction and is involved in the transition to heart failure by phosphorylating MRTFA and disrupting its interaction with actin.
Title: Protein Kinase N Promotes Stress-Induced Cardiac Dysfunction Through Phosphorylation of Myocardin-Related Transcription Factor A and Disruption of Its Interaction With Actin.
these findings strongly suggest that Msx1 cooperates with Pkn1 to down-regulate Myf5 and, therefore, prevent the differentiation of myogenic precursor cells.
Title: The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells.
results demonstrate that PKN1 functions as a developmentally active gatekeeper of AKT activity, thereby fine-tuning axonal outgrowth and presynaptic differentiation of Cgcs and subsequently the correct PF-PC synapse formation.
Title: Protein kinase N1 critically regulates cerebellar development and long-term function.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Ptger1 (e-PCR), detects polymorphism
- UniSTS:495971

Ptger1 (e-PCR), detects polymorphism
- UniSTS:144290

M-07712 (e-PCR)
- UniSTS:186282

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables histone H3T11 kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables histone binding	ISS Inferred from Sequence or Structural Similarity more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISS Inferred from Sequence or Structural Similarity more info
enables nuclear androgen receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear androgen receptor binding	ISS Inferred from Sequence or Structural Similarity more info
enables nuclear receptor coactivator activity	ISO Inferred from Sequence Orthology more info
enables nuclear receptor coactivator activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase C binding	IEA Inferred from Electronic Annotation more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within B cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within hyperosmotic response	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of B cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in post-translational protein modification	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of germinal center formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of immunoglobulin production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within renal system process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spleen development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in midbody	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: serine/threonine-protein kinase N1

Names: protein kinase C-like 1; protein kinase C-like PKN; protein-kinase C-related kinase 1; serine-threonine protein kinase N; serine/threonine kinase 3

NP_001186522.1

EC 2.7.11.13

NP_796236.2

EC 2.7.11.13

XP_030099481.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199593.1 → NP_001186522.1 serine/threonine-protein kinase N1 isoform 1

See identical proteins and their annotated locations for NP_001186522.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer protein (isoform 1).

Source sequence(s)

AC164432, AK089431, AK149649, CX568360

Consensus CDS

CCDS57627.1

UniProtKB/Swiss-Prot

P70268

Related

ENSMUSP00000116235.2, ENSMUST00000144258.8

Conserved Domains (5) summary

cd08687
Location:397 → 484

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:37 → 103

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11630
Location:126 → 197

HR1_PKN1_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N1

cd05589
Location:624 → 948

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:214 → 286

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
NM_177262.4 → NP_796236.2 serine/threonine-protein kinase N1 isoform 2

See identical proteins and their annotated locations for NP_796236.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1.

Source sequence(s)

AC164432, BC052923, CX568360

Consensus CDS

CCDS22459.1

UniProtKB/Swiss-Prot

P70268, Q3UEA6, Q7TST2, Q8BTL8

Related

ENSMUSP00000005616.9, ENSMUST00000005616.16

Conserved Domains (6) summary

PTZ00263
Location:616 → 939

PTZ00263; protein kinase A catalytic subunit; Provisional

cd08687
Location:392 → 479

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:32 → 98

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11630
Location:121 → 192

HR1_PKN1_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N1

cd05589
Location:619 → 943

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:209 → 281

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

84393162..84425819 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030243621.2 → XP_030099481.1 serine/threonine-protein kinase N1 isoform X1

Conserved Domains (5) summary

cd08687
Location:397 → 484

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:37 → 103

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11630
Location:126 → 197

HR1_PKN1_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N1

cd05589
Location:624 → 948

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:214 → 286

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases