GAS1 1,3-beta-glucanosyltransferase GAS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GAS1
Official Full Name: 1,3-beta-glucanosyltransferase GAS1
Primary source: SGD:S000004924
Locus tag: YMR307W
See related: AllianceGenome:SGD:S000004924; FungiDB:YMR307W; VEuPathDB:YMR307W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CWH52; GGP1
Summary: Enables 1,3-beta-glucanosyltransferase activity. Involved in several processes, including fungal-type cell wall organization; heterochromatin formation; and regulation of response to endoplasmic reticulum stress. Located in several cellular components, including COPII-coated ER to Golgi transport vesicle; cellular bud scar; and primary cell septum. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GAS1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (887003..888682)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast.
Title: GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast.
A screening aimed at identifying deletion mutants with reduced branching of beta-(1,3)-glucan through beta-(1,6)-glycosidic linkages among them revealed only two, the bgl2Delta and gas1Delta mutants, showing 15% and 70% reductions in the branching, respectively, compared to the wild-type strain.
Title: The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall.
ssays of heterologously expressed transglycosylases ScGas1, ScCrh1, and ScCrh2 from the yeast Saccharomyces cerevisiae, CaPhr1 and CaPhr2 from Candida albicans, and of a plant xyloglucan endotransglycosylase (XET) are demonstrated.
Title: Two Variants of a High-Throughput Fluorescent Microplate Assay of Polysaccharide Endotransglycosylases.
Analysis uncovers previously unsuspected functions for both Gas1 and Sas3 in DNA damage response and cell cycle regulation.
Title: Unexpected function of the glucanosyltransferase Gas1 in the DNA damage response linked to histone H3 acetyltransferases in Saccharomyces cerevisiae.
The results suggest that the dysfunction of Gas1 plays a positive role in the maintenance of rDNA integrity by decreasing PKA activity and inducing the accumulation of Msn2/4 in the nucleus.
Title: The β-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae.
Gas1p functions in cell wall formation, and is the primary glucan-remodeling enzyme required in vegetative growth and during reentry into the proliferative state.
Title: Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae.
Results suggest that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar, and reveal a new mechanism of protein immobilization at specific sites of the cell envelope.
Title: Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.
Because the catalytic activity of Gas1 is required for telomeric silencing, Gas1 localizes to the nuclear periphery, and Gas1 and Sir2 physically interact.
Title: The glucanosyltransferase Gas1 functions in transcriptional silencing.
Arl1p is involved in the anterograde transport from the Golgi to the cell surface of the glycosylphosphatidylinositol (GPI)-anchored plasma-membrane-resident protein Gas1p.
Title: Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane.
Functionally related Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall.
Title: Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 1,3-beta-glucanosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1,3-beta-glucanosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glucanosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall (1->3)-beta-D-glucan biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fungal-type cell wall organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in fungal-type cell wall organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall organization or biogenesis	IEA Inferred from Electronic Annotation more info
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in COPII-coated ER to Golgi transport vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud scar	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in fungal-type cell wall	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type cell wall	IDA Inferred from Direct Assay more info	PubMed
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in primary cell septum	IDA Inferred from Direct Assay more info	PubMed
located_in side of membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 1,3-beta-glucanosyltransferase GAS1

NP_014038.1

Beta-1,3-glucanosyltransferase required for cell wall assembly; also has role in transcriptional silencing; localizes to cell surface via glycosylphosphatidylinositol (GPI) anchor, also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate functions for Gas1p in DNA damage response and cell cycle regulation; protein demonstrates amyloid properties in vivo

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

887003..888682

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182818.1 → NP_014038.1 TPA: 1,3-beta-glucanosyltransferase GAS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014038.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0D4, P22146, P23151

UniProtKB/TrEMBL

A6ZN24, B3LMJ5, C7GPV6, C8ZFH7, N1P0S2

Conserved Domains (2) summary

pfam03198
Location:17 → 330

Glyco_hydro_72; Glucanosyltransferase

pfam07983
Location:390 → 452

X8; X8 domain