ALE1 lysophospholipid acyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ALE1
Official Full Name: lysophospholipid acyltransferase
Primary source: SGD:S000005701
Locus tag: YOR175C
See related: AllianceGenome:SGD:S000005701; FungiDB:YOR175C; VEuPathDB:YOR175C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LCA1; LPT1; SLC4
Summary: Enables 1-acylglycerol-3-phosphate O-acyltransferase activity and 1-acylglycerophosphocholine O-acyltransferase activity. Involved in glycerophospholipid biosynthetic process. Acts upstream of or within phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine. Located in endoplasmic reticulum and mitochondria-associated endoplasmic reticulum membrane contact site. Orthologous to several human genes including MBOAT2 (membrane bound O-acyltransferase domain containing 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ALE1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (659815..661674, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lysophospholipid acyltransferase-mediated formation of saturated glycerophospholipids maintained cell membrane integrity for hypoxic adaptation.
Title: Lysophospholipid acyltransferase-mediated formation of saturated glycerophospholipids maintained cell membrane integrity for hypoxic adaptation.
results reveal that ALE1 is involved in acyl chain transfer to the sn-1 position of HHPC in yeast.
Title: The membrane-bound O-acyltransferase Ale1 transfers an acyl moiety to newly synthesized 2-alkyl-sn-glycero-3-phosphocholine in yeast.
D146 and E297 act in conjunction with H382 as nucleophiles that attack the hydroxyl group in lysophospholipids in a general acid/base mechanism in Lpt1.
Title: Saccharomyces cerevisiae lysophospholipid acyltransferase, Lpt1, requires Asp146 and Glu297 for catalysis.
pology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
Title: Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
Identification of LCA1 provides an essential molecular tool for further study of Lands cycle in phosphatidylcholine turnover
Title: The yeast acylglycerol acyltransferase LCA1 is a key component of Lands cycle for phosphatidylcholine turnover.
in concert with Slc1, Lpt1 plays a central role in Phosphatidic acid biosynthesis, which is essential for cell viability.
Title: LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.
Slc1p and Slc4p may not only be active as 1-acylglycerol-3-phosphate O-acyltransferases but also be involved in fatty acid exchange at the sn-2-position of mature glycerophospholipids.
Title: SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast.
that a previously uncharacterized open reading frame, YOR175C, encodes the major acyl-CoA-dependent lysophosphatidylethanolamine acyltransferase activity in yeast
Title: Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 1-acylglycerophosphocholine O-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 1-acylglycerophosphoethanolamine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables 1-acylglycerophosphoserine O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glycerophospholipid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycerophospholipid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in glycerophospholipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid modification	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: lysophospholipid acyltransferase

NP_014818.1

EC 2.3.1.23

EC 2.3.1.51

EC 2.3.1.n6

EC 2.3.1.n7

Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

659815..661674 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183594.1 → NP_014818.1 TPA: lysophospholipid acyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014818.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2N1, Q03130, Q08548

UniProtKB/TrEMBL

A6ZP18, C7GN52, C8ZGT6, E9P8E9, N1P3B1

Conserved Domains (1) summary

COG5202
Location:1 → 563

COG5202; Predicted membrane protein [Function unknown]