Nfs1 nitrogen fixation gene 1 (S. cerevisiae) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nfs1provided by MGI
Official Full Name: nitrogen fixation gene 1 (S. cerevisiae)provided by MGI
Primary source: MGI:MGI:1316706
See related: Ensembl:ENSMUSG00000027618 AllianceGenome:MGI:1316706
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: m-Nfs1; m-Nfsl
Summary: Predicted to enable cysteine desulfurase activity and protein homodimerization activity. Involved in [4Fe-4S] cluster assembly and iron incorporation into metallo-sulfur cluster. Located in mitochondrial matrix and nucleus. Part of mitochondrial [2Fe-2S] assembly complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 52. Orthologous to human NFS1 (NFS1 cysteine desulfurase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in kidney adult (RPKM 43.8), adrenal adult (RPKM 39.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 77.34 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (155965569..155986106, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (156123637..156144202, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

NFS1 maintains the iron sulfur clusters in proteins that are essential for protecting them from oxidative damage; inactivating NFS1 or iron sulfur clusters can trigger ferroptosis, a non-apoptotic form of cell death, in cancer cells
Title: NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
While IFN-gamma alone induced Nfs1 protein instability, LPS triggered a delayed decline of Nfs1, rather involving transcriptional events or mRNA instability.
Title: Regulation of the cysteine desulfurase Nfs1 and the scaffold protein IscU in macrophages stimulated with interferon-gamma and lipopolysaccharide.
there is a mechanism that primarily dedicates m-Nfs1 to the biogenesis of mitochondrial Fe-S clusters in order to maintain cell survival
Title: RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AA987187 (e-PCR)
- UniSTS:159079

D2Mit48 (e-PCR), detects polymorphism
- UniSTS:129396

D2Dcr112 (e-PCR), detects polymorphism
- UniSTS:507590

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cysteine desulfurase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine desulfurase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables cysteine desulfurase activity	ISS Inferred from Sequence or Structural Similarity more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Mo-molybdopterin cofactor biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in [2Fe-2S] cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in [2Fe-2S] cluster assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in [4Fe-4S] cluster assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of [4Fe-4S] cluster assembly	ISO Inferred from Sequence Orthology more info
involved_in iron incorporation into metallo-sulfur cluster	IDA Inferred from Direct Assay more info	PubMed
involved_in iron incorporation into metallo-sulfur cluster	ISO Inferred from Sequence Orthology more info
involved_in iron-sulfur cluster assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in iron-sulfur cluster assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in molybdopterin cofactor biosynthetic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in molybdopterin cofactor metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
part_of iron-sulfur cluster assembly complex	NAS Non-traceable Author Statement more info	PubMed
part_of mitochondrial [2Fe-2S] assembly complex	IDA Inferred from Direct Assay more info	PubMed
part_of mitochondrial [2Fe-2S] assembly complex	ISO Inferred from Sequence Orthology more info
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	NAS Non-traceable Author Statement more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cysteine desulfurase

Names: cysteine desulfurase, mitochondrial; nifS-like (sic); nitrogen fixation gene, yeast homolog 1

NP_001407367.1

EC 2.8.1.7

NP_001407368.1

EC 2.8.1.7

NP_001407369.1

EC 2.8.1.7

NP_035041.2

EC 2.8.1.7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001420438.1 → NP_001407367.1 cysteine desulfurase isoform 2

Status: VALIDATED

Source sequence(s)

AL833786, BX649640

UniProtKB/TrEMBL

Q921V3
NM_001420439.1 → NP_001407368.1 cysteine desulfurase isoform 3

Status: VALIDATED

Source sequence(s)

AL833786, BX649640
NM_001420440.1 → NP_001407369.1 cysteine desulfurase isoform 4

Status: VALIDATED

Source sequence(s)

AL833786, BX649640

UniProtKB/TrEMBL

A6QRH3

Related

ENSMUSP00000105229.2, ENSMUST00000109600.2
NM_010911.3 → NP_035041.2 cysteine desulfurase isoform 1

See identical proteins and their annotated locations for NP_035041.2

Status: VALIDATED

Source sequence(s)

AL833786, BX649640

Consensus CDS

CCDS16962.1

UniProtKB/Swiss-Prot

Q8C6I5, Q9Z1J3

UniProtKB/TrEMBL

Q921V3

Related

ENSMUSP00000029147.10, ENSMUST00000029147.16

Conserved Domains (2) summary

PRK14012
Location:60 → 459

PRK14012; cysteine desulfurase; Provisional

pfam00266
Location:62 → 423

Aminotran_5; Aminotransferase class-V