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    Rnf145 ring finger protein 145 [ Mus musculus (house mouse) ]

    Gene ID: 74315, updated on 27-Dec-2024

    Summary

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    Official Symbol
    Rnf145provided by MGI
    Official Full Name
    ring finger protein 145provided by MGI
    Primary source
    MGI:MGI:1921565
    See related
    Ensembl:ENSMUSG00000019189 AllianceGenome:MGI:1921565
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    TMRF1; 3732413I11Rik
    Summary
    Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ERAD pathway and proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be located in endoplasmic reticulum membrane. Predicted to be active in endomembrane system. Is expressed in central nervous system; early conceptus; and genitourinary system. Orthologous to human RNF145 (ring finger protein 145). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Ubiquitous expression in thymus adult (RPKM 28.4), CNS E11.5 (RPKM 19.9) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Rnf145 in Genome Data Viewer
    Location:
    11 B1.1; 11 25.94 cM
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (44409486..44456347)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (44518793..44565520)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E5216 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44292046-44292346 Neighboring gene STARR-positive B cell enhancer ABC_E3941 Neighboring gene STARR-positive B cell enhancer ABC_E6969 Neighboring gene ubiquitin-like domain containing CTD phosphatase 1 Neighboring gene adenosine kinase pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E8391 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:44358832-44358985 Neighboring gene predicted gene, 51891 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:44391554-44391757 Neighboring gene RIKEN cDNA 4930597A21 gene Neighboring gene STARR-positive B cell enhancer mm9_chr11:44399542-44399843 Neighboring gene STARR-positive B cell enhancer ABC_E6970 Neighboring gene predicted gene, 39815 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44449840-44450140 Neighboring gene STARR-positive B cell enhancer ABC_E8392 Neighboring gene STARR-positive B cell enhancer ABC_E5217 Neighboring gene early B cell factor 1 Neighboring gene STARR-seq mESC enhancer starr_29281 Neighboring gene STARR-positive B cell enhancer ABC_E10669 Neighboring gene STARR-seq mESC enhancer starr_29286 Neighboring gene STARR-seq mESC enhancer starr_29287 Neighboring gene STARR-positive B cell enhancer ABC_E1821 Neighboring gene STARR-seq mESC enhancer starr_29288 Neighboring gene STARR-seq mESC enhancer starr_29289 Neighboring gene STARR-seq mESC enhancer starr_29290 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44713071-44713372 Neighboring gene STARR-seq mESC enhancer starr_29291 Neighboring gene STARR-positive B cell enhancer mm9_chr11:44763908-44764208 Neighboring gene STARR-seq mESC enhancer starr_29292

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP. Zhang L, et al. Elife, 2017 Oct 25. PMID 29068315, Free PMC Article
    2. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Menzies SA, et al. Elife, 2018 Dec 13. PMID 30543180, Free PMC Article
    3. Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene. Cook EC, et al. PLoS One, 2017. PMID 28231341, Free PMC Article
    4. Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst. Graham DB, et al. Nat Commun, 2015 Jul 21. PMID 26194095, Free PMC Article
    5. Identification of candidate maternal-effect genes through comparison of multiple microarray data sets. Mager J, et al. Mamm Genome, 2006 Sep. PMID 16964442

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. n the absence of both RNF145 and gp78, Hrd1, a third UBE2G2-dependent E3 ligase, partially regulates HMGCR activity. Our findings reveal a critical role for the sterol-responsive RNF145 in HMGCR regulation and elucidate the complexity of sterol-accelerated HMGCR degradation
      Title: The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.
    2. Mechanistic studies show that RNF145 triggers ubiquitination of SCAP on lysine residues within a cytoplasmic loop essential for COPII binding, potentially inhibiting its transport to Golgi and subsequent processing of SREBP-2.
      Title: Inhibition of cholesterol biosynthesis through RNF145-dependent ubiquitination of SCAP.
    3. we report the identification of RNF145 as an ER-resident E3 ubiquitin ligase that is transcriptionally controlled by LXR.
      Title: Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene.
    4. implicated in proteostasis of Nox2 complex
      Title: Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Gene trapped (1) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in ERAD pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in endomembrane system IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    RING finger protein 145
    NP_001160025.1
    NP_001350095.1
    NP_083138.2
    XP_006534420.1
    XP_006534421.1
    XP_030102252.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001166553.1 → NP_001160025.1  RING finger protein 145 isoform 2

      See identical proteins and their annotated locations for NP_001160025.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.
      Source sequence(s)
      AK139310, AL603913
      Consensus CDS
      CCDS48775.1
      UniProtKB/TrEMBL
      Q8BU61
      Related
      ENSMUSP00000098881.3, ENSMUST00000101327.3
      Conserved Domains (1) summary
      pfam13705
      Location:8 → 265
      TRC8_N; TRC8 N-terminal domain

    2. NM_001363166.1 → NP_001350095.1  RING finger protein 145 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AL603913
      Consensus CDS
      CCDS24565.1
      UniProtKB/Swiss-Prot
      Q5SWK7, Q8BXX5, Q9CXG1
      Conserved Domains (3) summary
      COG5243
      Location:520 → 627
      HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
      cd16684
      Location:533 → 575
      RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
      pfam13705
      Location:8 → 506
      TRC8_N; TRC8 N-terminal domain

    3. NM_028862.4 → NP_083138.2  RING finger protein 145 isoform 1

      See identical proteins and their annotated locations for NP_083138.2

      Status: VALIDATED

      Source sequence(s)
      AK014408, AK043002, AL603913, BB618481, CK624836
      Consensus CDS
      CCDS24565.1
      UniProtKB/Swiss-Prot
      Q5SWK7, Q8BXX5, Q9CXG1
      Related
      ENSMUSP00000019333.4, ENSMUST00000019333.10
      Conserved Domains (3) summary
      COG5243
      Location:520 → 627
      HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
      cd16684
      Location:533 → 575
      RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
      pfam13705
      Location:8 → 506
      TRC8_N; TRC8 N-terminal domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      44409486..44456347
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006534358.4 → XP_006534421.1  RING finger protein 145 isoform X1

      See identical proteins and their annotated locations for XP_006534421.1

      UniProtKB/Swiss-Prot
      Q5SWK7, Q8BXX5, Q9CXG1
      Conserved Domains (3) summary
      COG5243
      Location:520 → 627
      HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
      cd16684
      Location:533 → 575
      RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
      pfam13705
      Location:8 → 506
      TRC8_N; TRC8 N-terminal domain

    2. XM_006534357.3 → XP_006534420.1  RING finger protein 145 isoform X1

      See identical proteins and their annotated locations for XP_006534420.1

      UniProtKB/Swiss-Prot
      Q5SWK7, Q8BXX5, Q9CXG1
      Conserved Domains (3) summary
      COG5243
      Location:520 → 627
      HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
      cd16684
      Location:533 → 575
      RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
      pfam13705
      Location:8 → 506
      TRC8_N; TRC8 N-terminal domain

    3. XM_030246392.1 → XP_030102252.1  RING finger protein 145 isoform X2

      Conserved Domains (3) summary
      COG5243
      Location:408 → 515
      HRD1; HRD ubiquitin ligase complex, ER membrane component [Posttranslational modification, protein turnover, chaperones]
      cd16684
      Location:421 → 463
      RING-H2_RNF145; RING finger, H2 subclass, found in RING finger protein 145 (RNF145) and similar proteins
      pfam13705
      Location:1 → 394
      TRC8_N; TRC8 N-terminal domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q5SWK7.1 GenPept UniProtKB/Swiss-Prot:Q5SWK7

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