PCK1 phosphoenolpyruvate carboxykinase PCK1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PCK1
Official Full Name: phosphoenolpyruvate carboxykinase PCK1
Primary source: SGD:S000001805
Locus tag: YKR097W
See related: AllianceGenome:SGD:S000001805; FungiDB:YKR097W; VEuPathDB:YKR097W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: JPM2; PPC1
Summary: Enables phosphoenolpyruvate carboxykinase (ATP) activity. Involved in gluconeogenesis. Located in cytosol. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PCK1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (631152..632801)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1.
Title: PCK1 Deficiency Shortens the Replicative Lifespan of Saccharomyces cerevisiae through Upregulation of PFK1.
Functional evaluation of serine 252 of Saccharomyces cerevisiae PCK1 is reported.
Title: Functional evaluation of serine 252 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.
Arg457 is an important factor for nucleotide binding by S. cerevisiae PEP carboxykinase.
Title: Relevance of Arg457 for the nucleotide affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.
the electrostatic interaction between the guanidinium group of Arg70 and the carboxyl group of PEP is important for PEP binding by phosphoenolpyruvate (PEP) carboxykinase and for further steps in catalysis.
Title: Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: relevance of arginine 70 for catalysis.
neighboring Phe(416) contributes to provide a low polarity microenvironment suitable to keep the epsilon-NH(2) of Lys(213) in the unprotonated form
Title: Site-directed mutagenesis study of the microenvironment characteristics of Lys213 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase.
Introduction of a hydroxyl group in the lateral chain of the residue (Phe216Tyr)produces a substantial loss in the enzyme affinity for Mn(2+).
Title: Relevance of phenylalanine 216 in the affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase for Mn(II).

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables carboxy-lyase activity	IEA Inferred from Electronic Annotation more info
enables phosphoenolpyruvate carboxykinase (ATP) activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoenolpyruvate carboxykinase (ATP) activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoenolpyruvate carboxykinase (ATP) activity	IEA Inferred from Electronic Annotation more info
enables phosphoenolpyruvate carboxykinase (ATP) activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in gluconeogenesis	IEA Inferred from Electronic Annotation more info
involved_in gluconeogenesis	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphoenolpyruvate carboxykinase PCK1

NP_013023.3

EC 4.1.1.49

Phosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

631152..632801

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179887.3 → NP_013023.3 TPA: phosphoenolpyruvate carboxykinase PCK1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013023.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXF8, E9P963, P10963

UniProtKB/TrEMBL

A7A057, B3LRH2, B5VMN8, C7GXB4, C8ZCQ6, G2WIC5, N1P117

Conserved Domains (1) summary

cd00484
Location:31 → 545

PEPCK_ATP; Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and ...