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    NPA3 GTPase NPA3 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853535, updated on 9-Dec-2024

    Summary

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    Official Symbol
    NPA3
    Official Full Name
    GTPase NPA3
    Primary source
    SGD:S000003833
    Locus tag
    YJR072C
    See related
    AllianceGenome:SGD:S000003833; FungiDB:YJR072C; VEuPathDB:YJR072C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    EPA1; GPN1
    Summary
    Enables ATP hydrolysis activity and GTPase activity. Involved in mitotic sister chromatid cohesion and protein import into nucleus. Located in cytosol. Orthologous to human GPN1 (GPN-loop GTPase 1). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See NPA3 in Genome Data Viewer
    Location:
    chromosome: X
    Exon count:
    1
    Sequence:
    Chromosome: X; NC_001142.9 (570967..572124, complement)

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene nucleoside triphosphate pyrophosphohydrolase HAM1 Neighboring gene deoxyhypusine monooxygenase Neighboring gene bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase Neighboring gene Ran GTPase-binding protein MOG1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001142.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural insights into a new homodimeric self-activated GTPase family. Gras S, et al. EMBO Rep, 2007 Jun. PMID 17468740, Free PMC Article
    2. A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion. Alonso B, et al. Cell Cycle, 2011 Jun 1. PMID 21532343
    3. Npa3/ScGpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function. Guerrero-Serrano G, et al. Biochim Biophys Acta Mol Cell Res, 2017 Mar. PMID 27965115
    4. Translation initiation factor eIF1A rescues hygromycin B sensitivity caused by deleting the carboxy-terminal tail in the GPN-loop GTPase Npa3. Félix-Pérez T, et al. FEBS J, 2024 May. PMID 38431777
    5. Structure of GPN-Loop GTPase Npa3 and Implications for RNA Polymerase II Assembly. Niesser J, et al. Mol Cell Biol, 2015 Dec 28. PMID 26711263, Free PMC Article

    See all (70) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Synthetic negative genome screen of the GPN-loop GTPase NPA3 in Saccharomyces cerevisiae.
      Title: Synthetic negative genome screen of the GPN-loop GTPase NPA3 in Saccharomyces cerevisiae.
    2. Npa3/Gpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function.
      Title: Npa3/ScGpn1 carboxy-terminal tail is dispensable for cell viability and RNA polymerase II nuclear targeting but critical for microtubule stability and function.
    3. Npa3 has chaperone activity and interacts with hydrophobic peptide regions of RNA Polymerase II subunits that form interfaces in the assembled RNA Polymerase II complex.
      Title: Structure of GPN-Loop GTPase Npa3 and Implications for RNA Polymerase II Assembly.
    4. These results suggest that yGPN1 and yGPN2, yGPN3 proteins act at the molecular level in sister chromatid cohesion mechanism as a GPN|GPN complex.
      Title: Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN.
    5. Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase.
      Title: GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP hydrolysis activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in mitotic sister chromatid cohesion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in nucleocytoplasmic transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein import into nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 

    General protein information

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    Preferred Names
    GTPase NPA3
    NP_012606.3
    • Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9 Reference assembly

      Range
      570967..572124 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181730.3 → NP_012606.3  TPA: GTPase NPA3 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012606.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VWP2, P47122
      UniProtKB/TrEMBL
      A6ZQ30, B3LQF8, C7GMD2, C8ZBL9, G2WH76, N1P8R7
      Conserved Domains (1) summary
      cd17870
      Location:5 → 254
      GPN1; GPN-loop GTPase 1
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P47122.1

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