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    rap1 telomere-binding protein Rap1 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2540115, updated on 18-Sep-2024

    Summary

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    Official Symbol
    rap1
    Official Full Name
    telomere-binding protein Rap1
    Primary source
    PomBase:SPBC1778.02
    Locus tag
    SPOM_SPBC1778.02
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    lot2
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    Genomic context

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    See rap1 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    3
    Sequence:
    Chromosome: II; NC_003423.3 (3098271..3100698)

    Chromosome II - NC_003423.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene zuotin Neighboring gene 5S ribosomal RNA Neighboring gene ncRNA Neighboring gene NADH pyrophosphatase Neighboring gene guanyl-nucleotide exchange factor Lam2 Neighboring gene Spo4-Spo6 kinase complex regulatory subunit Spo6 Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003423.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. RNA-DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast. Hu Y, et al. Genetics, 2019 Oct. PMID 31405990, Free PMC Article
    2. Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells. Maestroni L, et al. Nucleic Acids Res, 2020 Apr 6. PMID 31980821, Free PMC Article
    3. Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast. Irie H, et al. PLoS Genet, 2019 Aug. PMID 31454352, Free PMC Article
    4. Fission yeast Rap1 homolog is a telomere-specific silencing factor and interacts with Taz1p. Park MJ, et al. Mol Cells, 2002 Apr 30. PMID 12018857
    5. The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope. Hu C, et al. Structure, 2019 Feb 5. PMID 30503780

    See all (45) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
      Title: The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.
    2. our data demonstrate that Rap1-Bqt4-dependent telomere association to nuclear envelope preserves telomere integrity in post-mitotic cells, preventing telomeric transcription and recombination
      Title: Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells.
    3. We demonstrate that deletion of Rap1 is protective in this context, preventing the growth crisis that is otherwise triggered by degradation of telomeric R-loops in survivors with linear chromosomes. These findings suggest that upregulation of telomere-engaged TERRA, or altered recruitment of shelterin components, can support telomerase-independent telomere maintenance.
      Title: RNA-DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast.
    4. Fission yeast cells lacking shelterin components Taz1 or Rap1 showed higher gross chromosomal rearrangements (GCRs) rates compared to wild-type, accumulating large chromosome deletions. Study results highlight unappreciated functions of the shelterin components Taz1 and Rap1 in maintaining genome stability, specifically by preventing non-telomeric GCRs.
      Title: Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.
    5. Rap1 is phosphorylated by CK2 at multiple sites, which promotes interactions with Bqt4 and Poz1. Phosphorylation at Ser496 was found to be crucial for both Rap1-Bqt4 and Rap1-Poz1 interactions.These mechanisms mediate proper telomere tethering to the NE and the formation of the silenced chromatin structure at chromosome ends.
      Title: Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.
    6. Telosome formation depends on the conserved telomeric proteins Taz1 and Rap1, and RNA. Rap1Delta telomeres lack detectable telosomes. Taz1 and Rap1 act together in organizing the telosome.
      Title: Fission yeast telosomes: non-canonical histone-containing chromatin structures dependent on shelterin and RNA.
    7. Whereas Rap1 hyper-phosphorylation observed in meiotic prophase does not have an apparent role in bouquet formation, the intrinsic negative charge of Rap1 is important for forming interactions with its binding partners.
      Title: Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation.
    8. fission yeast shelterin (shelterin subunits Poz1, Rap1 and Taz1 )maintains telomere length homeostasis by coordinating the differential arrival of leading (Polepsilon) and lagging (Polalpha) strand DNA polymerases at telomeres to modulate Rad3(ATR) association
      Title: Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.
    9. Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe
      Title: Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe.
    10. it is proposed that the telomere dissociation from the nuclear envelope promoted by Rap1 phosphorylation is critical for the fidelity of chromosome segregation in closed mitosis
      Title: Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables telomeric DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in meiotic attachment of telomeric heterochromatin to spindle pole body IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic attachment of telomeric heterochromatin to spindle pole body IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in mitotic telomere tethering at nuclear periphery IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protection from non-homologous end joining at telomere IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protection from non-homologous end joining at telomere IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere capping IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in telomere maintenance IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via telomere lengthening IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in chromosome, telomeric repeat region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in meiotic spindle pole body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nuclear telomere cap complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus HDA PubMed 
    is_active_in nucleus leading edge IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of shelterin complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of shelterin complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    telomere-binding protein Rap1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003423.3 Reference assembly

      Range
      3098271..3100698
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001022207.3 → NP_596285.1  telomere-binding protein Rap1 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_596285.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      Q96TL7, Q9UTW1, Q9UU15
      Conserved Domains (4) summary
      cd11655
      Location:122 → 170
      rap1_myb-like; DNA-binding modules of yeast Rap1 and related proteins
      cd11670
      Location:645 → 693
      Sp_RAP1_RCT; C-terminal domain of S. pombe RAP1 protein
      pfam09197
      Location:494 → 603
      Rap1-DNA-bind; Rap1, DNA-binding
      pfam16589
      Location:1 → 103
      BRCT_2; BRCT domain, a BRCA1 C-terminus domain
    Nucleotide Protein
    Heading Accession and Version

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