GRS1 glycine--tRNA ligase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GRS1
Official Full Name: glycine--tRNA ligase
Primary source: SGD:S000000325
Locus tag: YBR121C
See related: AllianceGenome:SGD:S000000325; FungiDB:YBR121C; VEuPathDB:YBR121C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables glycine-tRNA ligase activity and sequence-specific mRNA binding activity. Involved in DNA-templated transcription termination and mitochondrial glycyl-tRNA aminoacylation. Located in mitochondrion. Used to study Charcot-Marie-Tooth disease type 2D and spinal muscular atrophy. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease type 2D and autosomal dominant distal hereditary motor neuronopathy 5. Orthologous to human GARS1 (glycyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GRS1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (481364..483367, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A phylogenetic analysis further showed that GRS1 and GRS2 are paralogues that arose from a gene duplication event relatively recently, with GRS1 being the predecessor.
Title: An insertion peptide in yeast glycyl-tRNA synthetase facilitates both productive docking and catalysis of cognate tRNAs.
GRS2 rescued the growth defect of a GRS1 knockout strain when expressed at 37 degrees C, but not at 30 degrees C. These results suggest that GRS2 is actually an inducible gene that may function to rescue the activity of GRS1 under stress conditions.
Title: Saccharomyces cerevisiae possesses a stress-inducible glycyl-tRNA synthetase gene.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP:ATP adenylyltransferase activity	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity, acting on a tRNA	IEA Inferred from Electronic Annotation more info
enables glycine-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycine-tRNA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycine-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables glycine-tRNA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables sequence-specific mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription termination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in glycyl-tRNA aminoacylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial glycyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial glycyl-tRNA aminoacylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: glycine--tRNA ligase

NP_009679.2

EC 6.1.1.14

Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

481364..483367 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178469.2 → NP_009679.2 TPA: glycine--tRNA ligase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009679.2

Status: REVIEWED

UniProtKB/TrEMBL

B3LN34, B5VE81, C7GY06, G2W9C6, N1PAT2

Conserved Domains (1) summary

TIGR00389
Location:15 → 640

glyS_dimeric; glycyl-tRNA synthetase, dimeric type