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    Klf8 Kruppel-like transcription factor 8 [ Mus musculus (house mouse) ]

    Gene ID: 245671, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Klf8provided by MGI
    Official Full Name
    Kruppel-like transcription factor 8provided by MGI
    Primary source
    MGI:MGI:2442430
    See related
    Ensembl:ENSMUSG00000041649 AllianceGenome:MGI:2442430
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    BKLF3; ZNF74
    Summary
    Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in aggresome; cytosol; and nucleoplasm. Is expressed in early conceptus; inner cell mass; and liver. Orthologous to human KLF8 (KLF transcription factor 8). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Broad expression in CNS E18 (RPKM 3.7), whole brain E14.5 (RPKM 2.3) and 16 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Klf8 in Genome Data Viewer
    Location:
    X F3; X 68.46 cM
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (152008335..152181921)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (153225339..153398925)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_48111 Neighboring gene forkhead box R2 Neighboring gene Ras-related GTP binding B Neighboring gene STARR-seq mESC enhancer starr_48113 Neighboring gene STARR-seq mESC enhancer starr_48114 Neighboring gene STARR-seq mESC enhancer starr_48116 Neighboring gene STARR-seq mESC enhancer starr_48117 Neighboring gene STARR-seq mESC enhancer starr_48118 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene STARR-seq mESC enhancer starr_48121 Neighboring gene ubiquilin 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain. Dobrivojević M, et al. Gene, 2015 Oct 1. PMID 26071188
    2. A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers. Mehta TS, et al. Cell Res, 2009 Sep. PMID 19488069
    3. Krüppel-like factor KLF8 plays a critical role in adipocyte differentiation. Lee H, et al. PLoS One, 2012. PMID 23285057, Free PMC Article
    4. Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation. Wei H, et al. J Biol Chem, 2006 Jun 16. PMID 16617055
    5. A network of Krüppel-like Factors (Klfs). Klf8 is repressed by Klf3 and activated by Klf1 in vivo. Eaton SA, et al. J Biol Chem, 2008 Oct 3. PMID 18687676, Free PMC Article

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Klf8 is highly expressed in various regions of the mouse brain and in particular in the neurons, where it was localized in the cell nuclei.
      Title: Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain.
    2. KLF3 and KLF8 have overlapping roles in vivo and participate in the silencing of embryonic globin expression during development.
      Title: Generation of mice deficient in both KLF3/BKLF and KLF8 reveals a genetic interaction and a role for these factors in embryonic globin gene silencing.
    3. KLF8 is a key component of the transcription factor network that controls terminal differentiation during adipogenesis.
      Title: Krüppel-like factor KLF8 plays a critical role in adipocyte differentiation.
    4. PARP-1 as a novel KLF8-binding and -regulating protein and provided new insights into the mechanisms underlying the regulation of KLF8 nuclear localization, stability, and functions.
      Title: Identification of poly (ADP-ribose) polymerase-1 (PARP-1) as a novel Kruppel-like factor 8-interacting and -regulating protein.
    5. These results identified the KLF8 activation domain located between residues 101-260 where the well-conserved Q118 and Q248 are essential for recruiting p300 and PCAF to activate target gene transcription.
      Title: KLF8 recruits the p300 and PCAF co-activators to its amino terminal activation domain to activate transcription.
    6. KLF8 has two N terminal regulatory regions, one surrounding S165 and K171 and the other being two tandem ZFs, which are critical for the regulation of KLF8 nuclear localization and its cellular functions
      Title: A unique sequence in the N-terminal regulatory region controls the nuclear localization of KLF8 by cooperating with the C-terminal zinc-fingers.
    7. Klf8 is repressed by Klf3 and activated by Klf1 in vivo.
      Title: A network of Krüppel-like Factors (Klfs). Klf8 is repressed by Klf3 and activated by Klf1 in vivo.
    8. sumoylation of KLF8 negatively regulates its transcriptional activity and cellular functions
      Title: Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Gene trapped (2) 

    General gene information

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    Markers

    Homology

    Clone Names

    • A830097P10Rik

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-binding transcription repressor activity, RNA polymerase II-specific IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription repressor activity, RNA polymerase II-specific ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in negative regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in aggresome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in aggresome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    Krueppel-like factor 8
    Names
    Kruppel-like factor 8

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001357183.2 → NP_001344112.1  Krueppel-like factor 8

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AL671912
      Consensus CDS
      CCDS30481.1
      UniProtKB/Swiss-Prot
      Q8BLM0
      Related
      ENSMUSP00000044317.5, ENSMUST00000039545.5
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    2. NM_173780.6 → NP_776141.1  Krueppel-like factor 8

      See identical proteins and their annotated locations for NP_776141.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer transcript. Variants 1 and 2 encode the same protein.
      Source sequence(s)
      AL671912, AL672293, AL805962
      Consensus CDS
      CCDS30481.1
      UniProtKB/Swiss-Prot
      Q8BLM0
      Related
      ENSMUSP00000108193.3, ENSMUST00000112574.9
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      152008335..152181921
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017318503.1 → XP_017173992.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    2. XM_036161970.1 → XP_036017863.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    3. XM_017318505.3 → XP_017173994.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    4. XM_017318506.2 → XP_017173995.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    5. XM_006528859.4 → XP_006528922.1  Krueppel-like factor 8 isoform X3

      See identical proteins and their annotated locations for XP_006528922.1

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    6. XM_011247821.4 → XP_011246123.1  Krueppel-like factor 8 isoform X3

      See identical proteins and their annotated locations for XP_011246123.1

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    7. XM_006528852.5 → XP_006528915.1  Krueppel-like factor 8 isoform X2

      Conserved Domains (4) summary
      PHA03269
      Location:169 → 279
      PHA03269; envelope glycoprotein C; Provisional
      COG5048
      Location:358 → 428
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:362 → 384
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:420 → 442
      zf-C2H2; Zinc finger, C2H2 type

    8. XM_006528860.5 → XP_006528923.2  Krueppel-like factor 8 isoform X1

      Conserved Domains (4) summary
      COG5048
      Location:370 → 440
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:374 → 396
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam00096
      Location:432 → 454
      zf-C2H2; Zinc finger, C2H2 type
      cd21440
      Location:147 → 314
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    9. XM_030251350.2 → XP_030107210.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8

    10. XM_030251351.1 → XP_030107211.1  Krueppel-like factor 8 isoform X3

      UniProtKB/Swiss-Prot
      Q8BLM0
      Conserved Domains (4) summary
      COG5048
      Location:268 → 338
      COG5048; FOG: Zn-finger [General function prediction only]
      sd00017
      Location:272 → 294
      ZF_C2H2; C2H2 Zn finger [structural motif]
      pfam13465
      Location:316 → 341
      zf-H2C2_2; Zinc-finger double domain
      cd21440
      Location:45 → 212
      KLF8_N; N-terminal domain of Kruppel-like factor 8
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8BLM0.1 GenPept UniProtKB/Swiss-Prot:Q8BLM0

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