Dcx doublecortin [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dcxprovided by MGI
Official Full Name: doublecortinprovided by MGI
Primary source: MGI:MGI:1277171
See related: Ensembl:ENSMUSG00000031285 AllianceGenome:MGI:1277171
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Dbct
Summary: This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis, and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Expression: Biased expression in whole brain E14.5 (RPKM 80.1), CNS E18 (RPKM 75.8) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: X F2; X 64.89 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (142638838..142716392, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (143855842..143933396, complement)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A complex relation between levels of adult hippocampal neurogenesis and expression of the immature neuron marker doublecortin.
Title: A complex relation between levels of adult hippocampal neurogenesis and expression of the immature neuron marker doublecortin.
Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells.
Title: Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells.
Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES.
Title: Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES.
Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites.
Title: Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites.
NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet.
Title: NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet.
Results show that MyD88 deletion in microglia may negatively impact maturing neurons within the adult hippocampus and thus reward memories, suggesting a novel protective role for microglia in opioid addiction.
Title: Removal of microglial-specific MyD88 signaling alters dentate gyrus doublecortin and enhances opioid addiction-like behaviors.
The study demonstrated that the DCX was expressed in the olfactory bulb, rostral migratory stream, sub ventricular zone of lateral ventricle and the sub granular zone of the dentate gyrus, except for the cerebellum and tectum.
Title: Age-related changes in Ki-67 and DCX expression in the BALB/ c mouse (Mus Musculus) brain.
Authors found that 14-3-3epsilon binds to the microtubule binding protein, doublecortin (Dcx) in a phosphorylation dependent manner and that this binding increases the Dcx protein levels in the cell by interrupting ubiquitin based degradation.
Title: Regulation of neuronal morphogenesis by 14-3-3epsilon (Ywhae) via the microtubule binding protein, doublecortin.
MEIS2 associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber.
Title: MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1.
The regulation axis from microRNA-128-3p to doublecortin is critical for hippocampus-related contextual learning not only in wild type, but also in mice infused with Abeta-42.
Title: MicroRNA-128-3p impaired water maze learning by suppressing Doublecortin expression in both wild type and Aβ-42 infused mice.

Submit: New GeneRIF Correction See all GeneRIFs (68)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DXMit216 (e-PCR), detects polymorphism
- UniSTS:132105

Dcx (e-PCR), detects polymorphism
- UniSTS:525611

RH124178 (e-PCR)
- UniSTS:142838

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within axon extension	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axoneme assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within brain development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within central nervous system projection neuron axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within dendrite morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within hippocampus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within hippocampus development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within layer formation in cerebral cortex	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of collateral sprouting	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within pyramidal neuron development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of postsynapse assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retina development in camera-type eye	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in growth cone	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neuronal migration protein doublecortin

Names: doublecortex; doublin; lis-X; lissencephalin-X; lissencephaly, X-linked (doublecortin)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110222.1 → NP_001103692.1 neuronal migration protein doublecortin isoform a

See identical proteins and their annotated locations for NP_001103692.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same isoform (a).

Source sequence(s)

AB011678, AK164139, BC056391, CJ102759

Consensus CDS

CCDS53209.1

UniProtKB/Swiss-Prot

O88809, Q6E5A4

UniProtKB/TrEMBL

Q8C852

Related

ENSMUSP00000084570.4, ENSMUST00000087313.10

Conserved Domains (2) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2
NM_001110223.1 → NP_001103693.1 neuronal migration protein doublecortin isoform a

See identical proteins and their annotated locations for NP_001103693.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (a).

Source sequence(s)

AB011678, AK164139, BC056391

Consensus CDS

CCDS53209.1

UniProtKB/Swiss-Prot

O88809, Q6E5A4

UniProtKB/TrEMBL

Q8C852

Related

ENSMUSP00000033642.4, ENSMUST00000033642.10

Conserved Domains (2) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2
NM_001110224.1 → NP_001103694.1 neuronal migration protein doublecortin isoform b

See identical proteins and their annotated locations for NP_001103694.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and has an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in one aa less (isoform b), compared to isoform a.

Source sequence(s)

AB011678, AK164139, BC056391

Consensus CDS

CCDS53210.1

UniProtKB/TrEMBL

Q6PGI2, Q8C852

Related

ENSMUSP00000108472.2, ENSMUST00000112851.8

Conserved Domains (3) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2

cl40450
Location:284 → 343

KLF8_12_N; N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins
NM_001410009.1 → NP_001396938.1 neuronal migration protein doublecortin isoform b

Status: REVIEWED

Source sequence(s)

BX530055

UniProtKB/TrEMBL

Q6PGI2, Q8C852
NM_001410010.1 → NP_001396939.1 neuronal migration protein doublecortin isoform c

Status: REVIEWED

Source sequence(s)

BX530055

UniProtKB/TrEMBL

Q8C852, Q9CXL6
NM_001410011.1 → NP_001396940.1 neuronal migration protein doublecortin isoform e

Status: REVIEWED

Source sequence(s)

BX530055
NM_010025.2 → NP_034155.2 neuronal migration protein doublecortin isoform c

See identical proteins and their annotated locations for NP_034155.2

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, and has two alternate in-frame splice sites in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform c), compared to isoform a.

Source sequence(s)

AB011678, AK014264, BC057010, CA315795, CB525395, CD350374, CD802698

Consensus CDS

CCDS41157.1

UniProtKB/TrEMBL

Q8C852, Q9CXL6

Related

ENSMUSP00000108477.3, ENSMUST00000112856.3

Conserved Domains (3) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2

cl40450
Location:284 → 338

KLF8_12_N; N-terminal domain of Kruppel-like factor (KLF) 8, KLF12, and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000086.8 Reference GRCm39 C57BL/6J

Range

142638838..142716392 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006528698.4 → XP_006528761.1 neuronal migration protein doublecortin isoform X2

UniProtKB/TrEMBL

Q8C852

Conserved Domains (2) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2
XM_030251213.2 → XP_030107073.1 neuronal migration protein doublecortin isoform X2

UniProtKB/TrEMBL

Q8C852

Conserved Domains (2) summary

cd16112
Location:51 → 139

DCX1_DCX; Dublecortin-like domain 1 found in neuronal migration protein doublecortin (DCX)

cd17069
Location:176 → 259

DCX2; Dublecortin-like domain 2