Cul3 Cullin 3 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Cul3provided by FlyBase
Official Full Name: Cullin 3provided by FlyBase
Primary source: FLYBASE:FBgn0261268
Locus tag: Dmel_CG42616
See related: AllianceGenome:FB:FBgn0261268
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BG:DS07851.2; br34; CG11861; CG31829; CG42616; cul-3; Cul-3; CUL-3; cul3; CUL3; Cullin3; dCul-3; dCul3; DMcul-3; Dmel\CG42616; gft; gft/dCul-3; guftagu; l(2)06430; l(2)35Cd; l(2)br34; l35Cd; mds
Old locus tag: Dmel_CG11861; Dmel_CG31829
Summary: Enables ubiquitin ligase complex scaffold activity. Involved in several processes, including locomotor rhythm; negative regulation of signal transduction; and neuron projection morphogenesis. Acts upstream of with a positive effect on retrograde trans-synaptic signaling. Located in cytosol. Part of Cul3-RING ubiquitin ligase complex. Is active in postsynaptic cytosol. Colocalizes with female germline ring canal. Is expressed in several structures, including adult head; anterior endoderm; embryonic/larval midgut primordium; ganglia; and male germline cell. Used to study autism spectrum disorder. Human ortholog(s) of this gene implicated in pseudohypoaldosteronism. Orthologous to human CUL3 (cullin 3). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 35C5-35D1; 2-51 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (15265245..15272024, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (15265245..15272024, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neuronal knockdown of Cullin3 as a Drosophila model of autism spectrum disorder.
Title: Neuronal knockdown of Cullin3 as a Drosophila model of autism spectrum disorder.
Light Sampling via Throttled Visual Phototransduction Robustly Synchronizes the Drosophila Circadian Clock.
Title: Light Sampling via Throttled Visual Phototransduction Robustly Synchronizes the Drosophila Circadian Clock.
Study identified insomniac (inc), a putative adaptor for the Cullin-3 (Cul3) ubiquitin ligase complex, which together with Cul3 is essential for normal sleep regulation. Interestingly, Inc and Cul3 rapidly accumulate at postsynaptic compartments following acute receptor inhibition and are required for a local increase in mono-ubiquitination.
Title: Cul3 and insomniac are required for rapid ubiquitination of postsynaptic targets and retrograde homeostatic signaling.
CRL3(GCL) promotes primordial germ cell fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate.
Title: GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.
Data show that insomniac protein (INC) recognize its potential partners Cul3 and dGRASP.
Title: Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners.
A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
Title: A Cul-3-BTB ubiquitylation pathway regulates junctional levels and asymmetry of core planar polarity proteins.
these results establish inc and Cul3 as important new players in setting the sleep homeostat and a dopaminergic arousal pathway in Drosophila.
Title: Cul3 and the BTB adaptor insomniac are key regulators of sleep homeostasis and a dopamine arousal pathway in Drosophila.
Deregulation of CUL-3 in the clock neurons induces behavioral arrhythmicity.
Title: CULLIN-3 controls TIMELESS oscillations in the Drosophila circadian clock.
Extensive evolutionary conservation of insomniac and Cul3 suggests that protein degradation pathways may have a general role in governing the sleep and wakefulness of animals.
Title: insomniac and Cullin-3 regulate sleep and wakefulness in Drosophila.
Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein.
Title: Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

Dm0010 (e-PCR)
- UniSTS:74588

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin ligase complex scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin ligase complex scaffold activity	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotor rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of torso signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell fate determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IC Inferred by Curator more info	PubMed
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell proliferation involved in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of circadian sleep/wake cycle, sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reticulophagy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_positive_effect retrograde trans-synaptic signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory organ development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sleep	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm individualization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Cul3-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul3-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cul3-RING ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of cullin-RING ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with female germline ring canal	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with female germline ring canal	IPI Inferred from Physical Interaction more info	PubMed
is_active_in postsynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: cullin 3

Names: CG42616-PC; CG42616-PD; CG42616-PE; CG42616-PF; Cul3-PC; Cul3-PD; Cul3-PE; Cul3-PF; Cullin3; cullin; cullin-3; guftagu; medusa

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

15265245..15272024 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_165111.3 → NP_723908.2 cullin 3, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723908.2

Status: REVIEWED

UniProtKB/TrEMBL

C3KGQ1, Q9V475

Related

FBpp0291366

Conserved Domains (3) summary

smart00182
Location:421 → 563

CULLIN; Cullin

pfam00888
Location:35 → 670

Cullin; Cullin family

pfam10557
Location:705 → 765

Cullin_Nedd8; Cullin protein neddylation domain
NM_078849.3 → NP_523573.1 cullin 3, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523573.1

Status: REVIEWED

UniProtKB/TrEMBL

C3KGQ1, Q9V475

Related

FBpp0291367

Conserved Domains (3) summary

smart00182
Location:421 → 563

CULLIN; Cullin

pfam00888
Location:35 → 670

Cullin; Cullin family

pfam10557
Location:705 → 765

Cullin_Nedd8; Cullin protein neddylation domain
NM_165110.2 → NP_723907.1 cullin 3, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723907.1

Status: REVIEWED

UniProtKB/TrEMBL

C3KGQ1, Q9V475

Related

FBpp0291368

Conserved Domains (3) summary

smart00182
Location:421 → 563

CULLIN; Cullin

pfam00888
Location:35 → 670

Cullin; Cullin family

pfam10557
Location:705 → 765

Cullin_Nedd8; Cullin protein neddylation domain
NM_165112.2 → NP_723909.2 cullin 3, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723909.2

Status: REVIEWED

UniProtKB/TrEMBL

C3KGQ1, Q8IP45

Conserved Domains (3) summary

smart00182
Location:582 → 724

CULLIN; Cullin

pfam00888
Location:196 → 831

Cullin; Cullin family

pfam10557
Location:866 → 926

Cullin_Nedd8; Cullin protein neddylation domain