SGF73 deubiquitination module subunit SGF73 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SGF73
Official Full Name: deubiquitination module subunit SGF73
Primary source: SGD:S000003034
Locus tag: YGL066W
See related: AllianceGenome:SGD:S000003034; FungiDB:YGL066W; VEuPathDB:YGL066W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SCA7
Summary: Enables enzyme activator activity and structural molecule activity. Contributes to histone acetyltransferase activity. Involved in several processes, including mRNA export from nucleus; positive regulation of RNA polymerase II transcription preinitiation complex assembly; and regulation of protein localization to chromatin. Located in cytosol and nucleus. Part of DUBm complex; SAGA complex; and SLIK (SAGA-like) complex. Used to study spinocerebellar ataxia type 7. Human ortholog(s) of this gene implicated in spinocerebellar ataxia type 7. Orthologous to human ATXN7L1 (ataxin 7 like 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SGF73 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (377609..379582)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel ubiquitin-proteasome system regulation of Sgf73/ataxin-7 that maintains the integrity of the coactivator SAGA in orchestrating transcription.
Title: A novel ubiquitin-proteasome system regulation of Sgf73/ataxin-7 that maintains the integrity of the coactivator SAGA in orchestrating transcription.
Results show that the Sgf73p-mediated plasticity of gene expression is important for the ability of cells to tolerate stress and regulate proteostasis to survive under environmental uncertainty.
Title: SAGA DUBm-mediated surveillance regulates prompt export of stress-inducible transcripts for proteostasis.
The replicative lifespan-extending deletion of SGF73 results in altered ribosomal gene expression.
Title: The replicative lifespan-extending deletion of SGF73 results in altered ribosomal gene expression in yeast.
The 3D structure of SAGA complexes in which the subunit Sgf73 is partially or fully deleted to remove the deubiquitination module revealed the module localized in the vicinity of Gcn5 and Spt7.
Title: Mapping the deubiquitination module within the SAGA complex.
The Sgf73 subunit of the SAGA deubiquitinating module (DUBm) activates the catalytic Ubp8 subunit.
Title: Uncovering the role of Sgf73 in maintaining SAGA deubiquitinating module structure and activity.
A specific interaction between the proteasomal ATPase, Rpt2p and Sgf73p of the SAGA complex leads to the dissociation of the H2Bub1-deubiquitylating module from SAGA both in vitro and in vivo.
Title: Separation of a functional deubiquitylating module from the SAGA complex by the proteasome regulatory particle.
Sgf73 region (373-402 aa) is essential for heterochromatin boundary function.
Title: C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function.
Solution NMR and circular dichroism analysis of Sgf73(1-104) after zinc ion removal using chelation reagent EDTA demonstrated that zinc ion was required to maintain stable conformation of the zinc finger motif.
Title: Solution NMR characterization of Sgf73(1-104) indicates that Zn ion is required to stabilize zinc finger motif.
study reports the 1.90 angstrom resolution crystal structure of the deubiquitinating module (DUBm)- which contains Ubp8 bound to Sgf11, Sus1 and Sgf73- bound to ubiquitin aldehyde, as well as the 2.45 angstrom resolution structure of the uncomplexed DUBm
Title: Structural insights into the assembly and function of the SAGA deubiquitinating module.
Sgf73 provides a molecular scaffold to integrate the regulation of H2B ubiquitin levels, tethering of a gene to the NPC and export of mRNA
Title: Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to histone acetyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables structural molecule activity	IGI Inferred from Genetic Interaction more info	PubMed
enables structural molecule activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in RITS complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nucleocytoplasmic transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of protein localization to chromatin	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of DUBm complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IEA Inferred from Electronic Annotation more info
part_of SAGA complex	IPI Inferred from Physical Interaction more info	PubMed
part_of SLIK (SAGA-like) complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: deubiquitination module subunit SGF73

NP_011449.1

Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

377609..379582

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180931.1 → NP_011449.1 TPA: deubiquitination module subunit SGF73 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011449.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU75, P53165

UniProtKB/TrEMBL

A6ZUB0, B5VIQ7, N1P1W6

Conserved Domains (2) summary

pfam08313
Location:219 → 278

SCA7; zinc-binding domain

pfam18508
Location:59 → 100

zf_C2H2_13; Zinc finger domain