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    MPE1 cleavage polyadenylation factor subunit MPE1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853806, updated on 9-Dec-2024

    Summary

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    Official Symbol
    MPE1
    Official Full Name
    cleavage polyadenylation factor subunit MPE1
    Primary source
    SGD:S000001542
    Locus tag
    YKL059C
    See related
    AllianceGenome:SGD:S000001542; FungiDB:YKL059C; VEuPathDB:YKL059C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables pre-mRNA binding activity and ubiquitin protein ligase activity. Involved in protein polyubiquitination and termination of RNA polymerase II transcription. Located in cytosol. Part of mRNA cleavage and polyadenylation specificity factor complex. Is active in nucleus. Used to study cancer. Orthologous to human RBBP6 (RB binding protein 6, ubiquitin ligase). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See MPE1 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (328118..329443, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Bli1p Neighboring gene fructose-bisphosphate aldolase FBA1 Neighboring gene transcription initiation factor IIA subunit gamma Neighboring gene Nup120p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF. Rodríguez-Molina JB, et al. Mol Cell, 2022 Jul 7. PMID 35584695, Free PMC Article
    2. Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex. Hill CH, et al. Mol Cell, 2019 Mar 21. PMID 30737185, Free PMC Article
    3. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Vo LT, et al. Mol Cell Biol, 2001 Dec. PMID 11713271, Free PMC Article
    4. Regulation of the Ysh1 endonuclease of the mRNA cleavage/polyadenylation complex by ubiquitin-mediated degradation. Lee SD, et al. RNA Biol, 2020 May. PMID 32009536, Free PMC Article
    5. Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing. Kumar A, et al. Genes Dev, 2021 Nov 1. PMID 34593603, Free PMC Article

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.
      Title: Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.
    2. Determine the structure of Ysh1 bound to Mpe1 and the arrangement of subunits within CPFcore. Data suggest that the active mRNA 3' end processing machinery is a dynamic assembly that is licensed to cleave only when all protein factors come together at the polyadenylation site.
      Title: Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.
    3. Results show that three conserved domains (ubiquitin-like domain, a zinc knuckle, and a RING finger domain) of Mpe1 are important for normal yeast growth and mRNA 3'-end processing.
      Title: Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pre-mRNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pre-mRNA binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein polyubiquitination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein ubiquitination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein ubiquitination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in termination of RNA polymerase II transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mRNA cleavage and polyadenylation specificity factor complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cleavage polyadenylation factor subunit MPE1
    NP_012864.1
    • Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase; detects polyadenylation signal in nascent pre-mRNA and triggers 3' end formation of mRNA via cleavage and polyadenylation; binds pre-mRNA via zinc knuckle and RING finger; E3 ubiquitin ligase involved in polyubiquitination of endonuclease Ysh1p; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and a RING finger domain; relocalizes to cytosol in response to hypoxia; homolog of human RBBP6

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      328118..329443 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179625.1 → NP_012864.1  TPA: cleavage polyadenylation factor subunit MPE1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012864.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXM8, P35728
      UniProtKB/TrEMBL
      A6ZZQ7, B3LR32, B5VM98, C7GP15, C8ZCB3, G2WHX3, N1P0L5
      Conserved Domains (1) summary
      COG5222
      Location:1 → 441
      COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P35728.1

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