PEX6 AAA family ATPase peroxin 6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PEX6
Official Full Name: AAA family ATPase peroxin 6
Primary source: SGD:S000005273
Locus tag: YNL329C
See related: AllianceGenome:SGD:S000005273; FungiDB:YNL329C; VEuPathDB:YNL329C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAS8
Summary: Enables ATP hydrolysis activity. Involved in protein import into peroxisome matrix, receptor recycling and protein unfolding. Located in cytosol and peroxisome. Used to study peroxisomal biogenesis disorder. Human ortholog(s) of this gene implicated in Heimler syndrome 2; Peroxisome biogenesis disorder 4B; and peroxisome biogenesis disorder 4A. Orthologous to human PEX6 (peroxisomal biogenesis factor 6). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PEX6 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (19541..22633, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pex1/Pex6 heterohexameric motor is a protein translocase that unfolds Pex15 in a pore-loop-dependent and ATP-hydrolysis-dependent manner
Title: The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading.
the Pex1/Pex6 complex has roles in matrix protein import; peroxisomes in Saccharomyces cerevisiae multiply mainly by growth and division
Title: Reevaluation of the role of Pex1 and dynamin-related proteins in peroxisome membrane biogenesis.
Pex1 and Pex6 play a role in peroxisomal matrix protein import
Title: Yeast pex1 cells contain peroxisomal ghosts that import matrix proteins upon reintroduction of Pex1.
ATP hydrolysis results in a pumping motion of the complex, suggesting that Pex1/6 function involves substrate translocation through its central channel
Title: Molecular snapshots of the Pex1/6 AAA+ complex in action.
the Pex1/Pex6 complex has a role in peroxisomal protein import analogous to p97 in ER-associated protein degradation
Title: Unique double-ring structure of the peroxisomal Pex1/Pex6 ATPase complex revealed by cryo-electron microscopy.
Mutants of PEX1, PEX6, and PEX15 accumulate ubiquitinated receptors at the peroxisomal membrane. This accumulation has been suggested to trigger pexophagy in mammalian cells.
Title: Deficiency of the exportomer components Pex1, Pex6, and Pex15 causes enhanced pexophagy in Saccharomyces cerevisiae.
Data show an ATP-dependent assembly of the heteromeric Pex1p-Pex6p-complex of the peroxisomal matrix protein import machinery.
Title: ATP-dependent assembly of the heteromeric Pex1p-Pex6p-complex of the peroxisomal matrix protein import machinery.
These results provide evidence that in a deletion of PEX6 increases acetic acid-mediated necrotic cell death in Saccharomyces cerevisiae.
Title: Loss of peroxisome function triggers necrosis.
Pex1p and Pex6p from Saccharomyces cerevisiae do interact in vivo
Title: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p.
Pex6 protein mediates the ATP-dependent dislocation of the peroxisomal targeting signal-1 (PTS1) receptor from the peroxisomal membrane to the cytosol.
Title: Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in peroxisome organization	IEA Inferred from Electronic Annotation more info
involved_in protein import into peroxisome matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into peroxisome matrix	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into peroxisome matrix, receptor recycling	IDA Inferred from Direct Assay more info	PubMed
involved_in protein import into peroxisome matrix, receptor recycling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein import into peroxisome matrix, receptor recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein unfolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein unfolding	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in peroxisomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisomal membrane	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: AAA family ATPase peroxin 6

NP_014070.1

AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

19541..22633 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183167.1 → NP_014070.1 TPA: AAA family ATPase peroxin 6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014070.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0L8, P33760

UniProtKB/TrEMBL

A6ZSF3, G2WLE2, N1NWS0

Conserved Domains (2) summary

COG0464
Location:540 → 1017

SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]

cd19527
Location:740 → 899

RecA-like_PEX6_r2; second of two ATPase domains of Peroxisomal biogenesis factor 6 (PEX6)