Tssk3 testis-specific serine kinase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tssk3provided by MGI
Official Full Name: testis-specific serine kinase 3provided by MGI
Primary source: MGI:MGI:1929914
See related: Ensembl:ENSMUSG00000000411 AllianceGenome:MGI:1929914
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tsk-3; Stk22c; Stk22d; Tssk-3; Tssk3b; 1700014N07Rik; 4930594I21Rik
Summary: Enables magnesium ion binding activity; manganese ion binding activity; and protein serine/threonine kinase activity. Involved in spermatid development. Is active in sperm flagellum. Is expressed in germ cell of testis; spermatid; spermatocyte; and testis. Orthologous to human TSSK3 (testis specific serine kinase 3). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Restricted expression toward testis adult (RPKM 112.4) See more
Orthologs: human all
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Genomic context

Location:: 4 D2.2; 4 63.26 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (129382801..129385523, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (129489008..129491730, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility.
Title: Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility.
PDK1-induced phosphorylation increased in vitro TSSK3 kinase activity, suggesting that TSSK3 can be regulated in the same way as AGC kinase family members. The peptide sequence RRSSSY containing Ser5 is identified as a target for TSSK3 phosphorylation
Title: Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables manganese ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	NAS Non-traceable Author Statement more info	PubMed
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in sperm capacitation	NAS Non-traceable Author Statement more info	PubMed
involved_in spermatid development	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in sperm flagellum	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: testis-specific serine/threonine-protein kinase 3

Names: serine/threonine kinase 22C (spermiogenesis associated); serine/threonine-protein kinase 22C; spermiogenesis associated serine/threonine kinase 22D; testis-specific kinase 3; testis-specific serine/threonine kinase 3b

NP_536690.1

EC 2.7.11.1

XP_006503317.1

EC 2.7.11.1

XP_030109549.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080442.2 → NP_536690.1 testis-specific serine/threonine-protein kinase 3

Status: VALIDATED

Source sequence(s)

AK019840

Consensus CDS

CCDS18693.1

UniProtKB/Swiss-Prot

Q9D2E1, Q9JL98

Related

ENSMUSP00000000421.6, ENSMUST00000000421.6

Conserved Domains (1) summary

cd14163
Location:9 → 265

STKc_TSSK3-like; Catalytic domain of testis-specific serine/threonine kinase 3 and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

129382801..129385523 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030253689.1 → XP_030109549.1 testis-specific serine/threonine-protein kinase 3 isoform X1

UniProtKB/Swiss-Prot

Q9D2E1, Q9JL98

Conserved Domains (1) summary

cd14163
Location:9 → 265

STKc_TSSK3-like; Catalytic domain of testis-specific serine/threonine kinase 3 and similar proteins
XM_006503254.4 → XP_006503317.1 testis-specific serine/threonine-protein kinase 3 isoform X2

Conserved Domains (1) summary

cl21453
Location:1 → 190

PKc_like; Protein Kinases, catalytic domain