VAC14 Vac14p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VAC14
Official Full Name: Vac14p
Primary source: SGD:S000004378
Locus tag: YLR386W
See related: AllianceGenome:SGD:S000004378; FungiDB:YLR386W; VEuPathDB:YLR386W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein-macromolecule adaptor activity. Involved in several processes, including cellular hyperosmotic response; phosphatidylinositol biosynthetic process; and protein localization to vacuolar membrane. Located in fungal-type vacuole membrane. Part of PAS complex. Human ortholog(s) of this gene implicated in striatonigral degeneration. Orthologous to human VAC14 (VAC14 component of PIKFYVE complex). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See VAC14 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (893628..896270)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.
The authors show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
Title: VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.
Vac14p-Fig4p complex controls the hyperosmotic shock-induced increase in phosphatidylinositol 3,5-diphosphate levels.
Title: The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylinositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to vacuolar membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of PAS complex	IBA Inferred from Biological aspect of Ancestor more info
part_of PAS complex	IDA Inferred from Direct Assay more info	PubMed
part_of PAS complex	IEA Inferred from Electronic Annotation more info
part_of PAS complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Vac14p

NP_013490.3

Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

893628..896270

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182275.3 → NP_013490.3 TPA: Vac14p [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZ21, Q06708

UniProtKB/TrEMBL

B5VNS3, C7GXJ0, C8ZDZ5, N1P7A8

Conserved Domains (3) summary

sd00044
Location:87 → 115

HEAT; HEAT repeat [structural motif]

pfam11916
Location:573 → 746

Vac14_Fig4_bd; Vacuolar protein 14 C-terminal Fig4p binding

pfam12755
Location:59 → 156

Vac14_Fab1_bd; Vacuolar 14 Fab1-binding region