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    alp7 kinetochore-associated protein Alp7 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2543382, updated on 18-Sep-2024

    Summary

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    Official Symbol
    alp7
    Official Full Name
    kinetochore-associated protein Alp7
    Primary source
    PomBase:SPAC890.02c
    Locus tag
    SPOM_SPAC890.02C
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    mia1
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    Genomic context

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    See alp7 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    1
    Sequence:
    Chromosome: I; NC_003424.3 (5002400..5004485, complement)

    Chromosome I - NC_003424.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene RhoGAP family GTPase-activator protein Neighboring gene ncRNA Neighboring gene GTPase-activating protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene serine/threonine protein kinase Ppk16 Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003424.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Astral microtubules monitor metaphase spindle alignment in fission yeast. Oliferenko S, et al. Nat Cell Biol, 2002 Oct. PMID 12360293
    2. The spindle pole bodies facilitate nuclear envelope division during closed mitosis in fission yeast. Zheng L, et al. PLoS Biol, 2007 Jul. PMID 17579515, Free PMC Article
    3. The fission yeast TACC protein Mia1p stabilizes microtubule arrays by length-independent crosslinking. Thadani R, et al. Curr Biol, 2009 Nov 17. PMID 19879140
    4. Csi1p recruits alp7p/TACC to the spindle pole bodies for bipolar spindle formation. Zheng F, et al. Mol Biol Cell, 2014 Sep 15. PMID 25057016, Free PMC Article
    5. Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation. Sato M, et al. Mol Biol Cell, 2004 Apr. PMID 14742702, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. clustered five amino acid residues within the TACC domain required for spindle pole body localization
      Title: Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast.
    2. The ability of Alp7/14 to accelerate and bias GTP-tubulin exchange at microtubule plus ends allows it to generate long-lived, fast-growing microtubules at very low cellular free tubulin concentrations.
      Title: Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.
    3. We identified an alp7 mutant that had specific defects in binding to the Klp5-Klp6-PP1 complex but with normal localisation to the microtubule and kinetochore.
      Title: Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.
    4. Csi1p serves as a linking molecule tethering spindle-stabilizing factors such as alp7p to the spindle pole body for promoting bipolar spindle assembly.
      Title: Csi1p recruits alp7p/TACC to the spindle pole bodies for bipolar spindle formation.
    5. CDK phosphorylates the Alp7-Alp14 complex to localize it to the nucleus.
      Title: CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly.
    6. An Ndc80 loop plays sequential roles in spindle-kinetochore attachment by connecting the Ndc80 complex to Dis1/TOG and Alp7/TACC-Alp14/TOG.
      Title: The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.
    7. Mia1p/Alp7p, a transforming acidic coiled-coil (TACC) protein, functions as a length-independent MT crosslinker.
      Title: The fission yeast TACC protein Mia1p stabilizes microtubule arrays by length-independent crosslinking.
    8. results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC-Alp14/TOG complex through the coordinated interplay of Ran and cyclin-dependent kinase.
      Title: Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast.
    9. When the Crm1p-dependent nuclear export signal of Mia1p is disabled, Mia1p accumulates in the nucleus while its partner protein Alp14p/TOG is restricted to the cytoplasm
      Title: Nucleocytoplasmic shuttling of the TACC protein Mia1p/Alp7p is required for remodeling of microtubule arrays during the cell cycle.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in astral microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in attachment of mitotic spindle microtubules to kinetochore EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in attachment of mitotic spindle microtubules to kinetochore IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cytoplasmic microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic centromere clustering IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in microtubule nucleation by interphase microtubule organizing center IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic chromosome movement towards spindle pole IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle elongation (spindle phase three) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle formation (spindle phase one) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport along microtubule to kinetochore IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of TACC/TOG complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasmic microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasmic microtubule bundle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasmic microtubule plus-end IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in equatorial microtubule organizing center IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in microtubule minus-end IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle pole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle pole body HDA PubMed 
    is_active_in mitotic spindle pole body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of outer kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in static microtubule bundle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    kinetochore-associated protein Alp7

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003424.3 Reference assembly

      Range
      5002400..5004485 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001020249.3 → NP_594820.1  kinetochore-associated protein Alp7 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_594820.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      Q9URY2, Q9UU56
      Conserved Domains (1) summary
      pfam12709
      Location:316 → 407
      Kinetocho_Slk19; Central kinetochore-associated
    Nucleotide Protein
    Heading Accession and Version

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