Zp2 zona pellucida glycoprotein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Zp2provided by MGI
Official Full Name: zona pellucida glycoprotein 2provided by MGI
Primary source: MGI:MGI:99214
See related: Ensembl:ENSMUSG00000030911 AllianceGenome:MGI:99214
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Zp-2
Summary: This gene encodes a member of the zona pellucida family of glycoproteins that play an important role in the survival of growing oocytes, successful fertilization and the passage of early embryos through the oviduct. The encoded preproprotein undergoes proteolytic processing to generate the mature polypeptide that is incorporated into the extracellular matrix surrounding mouse oocytes. Mice lacking the encoded protein develop defective zonae pellucidae that disrupt folliculogenesis, fertility and development. [provided by RefSeq, Sep 2016]
Expression: Restricted expression toward ovary adult (RPKM 15.6) See more
Orthologs: human all
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Genomic context

Location:: 7 F2; 7 64.38 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (119725985..119744514, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (120126762..120145291, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
Title: Identification of a heterozygous variant of ZP2 as a novel cause of empty follicle syndrome in humans and mice.
The central peptide of ZP2 (190-505) is immunogenic and contains zona pellucida-specific epitopes.
Title: The bioactivities of the central segment of Zp2 polypeptide.
ZP2(51-149) sperm-binding domain is necessary for mouse gamete recognition and penetration through the zona pellucida.
Title: A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans.
Studies indicate that eggs from mZP2-/- and mZP3-/- females lack a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts.
Title: Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.
Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
Title: Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease.
Title: Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
Cytoplasmic tails are necessary to prevent intracellular oligomerization while ensuring incorporation of processed ZP2 and ZP3 into the zona pellucida.
Title: ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.
The granulosa cells contribute more to the mZP2 mRNA synthesis than oocyte does.
Title: Mouse granulosa cells contribute more to the mRNA synthesis of mZP2 than oocyte does.
data indicate that sperm-egg recognition depends on the cleavage status of ZP2 and that binding at the surface of the zona is not sufficient to induce sperm acrosome exocytosis
Title: Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein.
characterization of native mouse zona pellucida proteins by mass spectrometry
Title: Structural characterization of native mouse zona pellucida proteins using mass spectrometry.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Spontaneous (1) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Zp2 (e-PCR), detects polymorphism
- UniSTS:256834

Zp2 (e-PCR), detects polymorphism
- UniSTS:525728

Zp2 (e-PCR), detects polymorphism
- UniSTS:144844

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acrosin binding	IBA Inferred from Biological aspect of Ancestor more info
enables acrosin binding	IEA Inferred from Electronic Annotation more info
enables acrosin binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of egg coat	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of egg coat	ISO Inferred from Sequence Orthology more info
enables structural constituent of egg coat	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in binding of sperm to zona pellucida	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within binding of sperm to zona pellucida	IDA Inferred from Direct Assay more info	PubMed
involved_in binding of sperm to zona pellucida	IDA Inferred from Direct Assay more info	PubMed
involved_in prevention of polyspermy	IBA Inferred from Biological aspect of Ancestor more info
involved_in prevention of polyspermy	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in collagen-containing extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in egg coat	IBA Inferred from Biological aspect of Ancestor more info
located_in egg coat	ISO Inferred from Sequence Orthology more info
located_in egg coat	ISS Inferred from Sequence or Structural Similarity more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in multivesicular body	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: zona pellucida sperm-binding protein 2

Names: zona pellucida glycoprotein ZP2; zona pellucida protein A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001374631.1 → NP_001361560.1 zona pellucida sperm-binding protein 2 isoform 2 preproprotein

Status: REVIEWED

Source sequence(s)

AC122853

Consensus CDS

CCDS90322.1

UniProtKB/TrEMBL

Q3UX44, Q6IR45

Related

ENSMUSP00000147097.2, ENSMUST00000208874.2

Conserved Domains (1) summary

smart00241
Location:365 → 630

ZP; Zona pellucida (ZP) domain
NM_011775.7 → NP_035905.1 zona pellucida sperm-binding protein 2 isoform 1 preproprotein

See identical proteins and their annotated locations for NP_035905.1

Status: REVIEWED

Source sequence(s)

AK135903, AK138699, DV657934

Consensus CDS

CCDS21792.1

UniProtKB/Swiss-Prot

P20239

UniProtKB/TrEMBL

Q6IR45

Related

ENSMUSP00000033207.5, ENSMUST00000033207.6

Conserved Domains (1) summary

smart00241
Location:365 → 630

ZP; Zona pellucida (ZP) domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

119725985..119744514 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507590.4 → XP_006507653.1 zona pellucida sperm-binding protein 2 isoform X1

UniProtKB/TrEMBL

Q6IR45

Conserved Domains (1) summary

smart00241
Location:237 → 502

ZP; Zona pellucida (ZP) domain