Prkcq protein kinase C, theta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkcqprovided by MGI
Official Full Name: protein kinase C, thetaprovided by MGI
Primary source: MGI:MGI:97601
See related: Ensembl:ENSMUSG00000026778 AllianceGenome:MGI:97601
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkcq; PKC-0; PKCtheta; PKC-theta; A130035A12Rik
Summary: Enables diacylglycerol-dependent serine/threonine kinase activity. Involved in several processes, including positive regulation of T-helper 17 type immune response; regulation of cell-cell adhesion; and regulation of gene expression. Acts upstream of or within several processes, including positive regulation of CD4-positive, alpha-beta T cell proliferation; positive regulation of interleukin-2 production; and protein phosphorylation. Located in cytoplasm and immunological synapse. Is expressed in several structures, including adipose tissue; genitourinary system; gut; limb; and nervous system. Used to study retinal detachment. Orthologous to human PRKCQ (protein kinase C theta). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in testis adult (RPKM 34.4), thymus adult (RPKM 13.0) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 A1; 2 8.42 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (11176922..11306033)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (11171905..11301226)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Distinct subcellular localisation of intramyocellular lipids and reduced PKCepsilon/PKCtheta; activity preserve muscle insulin sensitivity in exercise-trained mice.
Title: Distinct subcellular localisation of intramyocellular lipids and reduced PKCε/PKCθ activity preserve muscle insulin sensitivity in exercise-trained mice.
Protein kinase C-theta knockout decreases serum IL-10 levels and inhibits insulin secretion from islet beta cells.
Title: Protein kinase C-θ knockout decreases serum IL-10 levels and inhibits insulin secretion from islet β cells.
Annexin A5 is essential for PKCtheta translocation during T-cell activation.
Title: Annexin A5 is essential for PKCθ translocation during T-cell activation.
Targeting PKCtheta Promotes Satellite Cell Self-Renewal.
Title: Targeting PKCθ Promotes Satellite Cell Self-Renewal.
Lack of PKCtheta Promotes Regenerative Ability of Muscle Stem Cells in Chronic Muscle Injury.
Title: Lack of PKCθ Promotes Regenerative Ability of Muscle Stem Cells in Chronic Muscle Injury.
These findings demonstrate that PKCtheta; plays an important role in a positive feedback regulatory loop that modulates TCR-proximal signaling and, moreover, provide a mechanistic explanation for earlier reports that documented an important role for PKCtheta; in T cell Ca(2+) signaling. This PKCtheta;-Zap70 interaction could potentially serve as a promising and highly selective immunosuppressive drug target in autoimmu...
Title: Phosphotyrosine-dependent interaction between the kinases PKCθ and Zap70 promotes proximal TCR signaling.
The authors established that OX40L/OX40 interaction was required and sufficient to induce regulatory T-cell proliferation in vivo independent of PKC- status. Although PKC- is dispensable for T-cell receptor-independent regulatory T-cell proliferation per se, it is essential for optimum IL-2 production by effector T-cells.
Title: PKC-ѳ is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells.
PKC-theta; is a regulator of lipid-induced insulin resistance in skeletal muscle.
Title: Mice lacking PKC-θ in skeletal muscle have reduced intramyocellular lipid accumulation and increased insulin responsiveness in skeletal muscle.
Comparison with expanded cord blood-derived CD4(+)CD25(hi) tTreg and expanded Teffs from the same donors indicate that iTreg are intermediate between expanded CD4(+)CD25(hi) tTregs and Teffs, whereas modulation of suppressive activities by PKC-theta; and Dlgh1 signaling pathways are shared.
Title: Human in vitro-induced regulatory T cells display Dlgh1dependent and PKC-θ restrained suppressive activity.
PKCtheta; selectively inactivates the negative regulator of F-actin generation, Coronin 1A, at the center of the T cell interface with the antigen presenting cell (APC). This allows for effective generation of the large actin-based lamellum required for recruitment of the Notch-processing membrane metalloproteinase ADAM10.
Title: PKCθ links proximal T cell and Notch signaling through localized regulation of the actin cytoskeleton.

Submit: New GeneRIF Correction See all GeneRIFs (109)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (2)
Endonuclease-mediated (3)
Spontaneous (1) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkcq (e-PCR), detects polymorphism
- UniSTS:144199

Pkcq (e-PCR)
- UniSTS:144200

AW494342 (e-PCR)
- UniSTS:178568

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of CD4-positive, alpha-beta T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of T cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell chemotaxis	IEA Inferred from Electronic Annotation more info
involved_in cell chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in inflammatory response	IEA Inferred from Electronic Annotation more info
acts_upstream_of interleukin-2 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intracellular signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in membrane protein ectodomain proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of T cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of T cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of insulin receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of CD4-positive, alpha-beta T cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of NF-kappaB transcription factor activity	ISA Inferred from Sequence Alignment more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of T cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of T cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of T-helper 17 type immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of T-helper 2 cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of filopodium assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-17 production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of interleukin-2 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-4 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere capping	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere maintenance	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of telomere maintenance via telomerase	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of platelet aggregation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in aggresome	IEA Inferred from Electronic Annotation more info
located_in aggresome	ISO Inferred from Sequence Orthology more info
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in immunological synapse	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein kinase C theta type

Names: PKC theta; nPKC-theta

NP_001405286.1

EC 2.7.11.13

NP_001405287.1

EC 2.7.11.13

NP_001405288.1

EC 2.7.11.13

NP_032885.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001418357.1 → NP_001405286.1 protein kinase C theta type isoform 3

Status: VALIDATED

Source sequence(s)

AL928715, AL929020

UniProtKB/TrEMBL

F8WGT6
NM_001418358.1 → NP_001405287.1 protein kinase C theta type isoform 4

Status: VALIDATED

Source sequence(s)

AL928715, AL929020

UniProtKB/TrEMBL

F8WGT6
NM_001418359.1 → NP_001405288.1 protein kinase C theta type isoform 5

Status: VALIDATED

Source sequence(s)

AL929020

UniProtKB/TrEMBL

Q920N8

Related

ENSMUST00000195628.2
NM_008859.3 → NP_032885.1 protein kinase C theta type isoform 1

See identical proteins and their annotated locations for NP_032885.1

Status: VALIDATED

Source sequence(s)

AL928715, AL929020

Consensus CDS

CCDS15682.1

UniProtKB/Swiss-Prot

Q02111

UniProtKB/TrEMBL

A6H667, F8WGT6

Related

ENSMUSP00000028118.4, ENSMUST00000028118.9

Conserved Domains (3) summary

smart00220
Location:380 → 634

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05619
Location:374 → 704

STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta

pfam00130
Location:232 → 284

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

11176922..11306033

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_178075.2: Suppressed sequence

Description

NM_178075.2: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.