URA2 bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: URA2
Official Full Name: bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase
Primary source: SGD:S000003666
Locus tag: YJL130C
See related: AllianceGenome:SGD:S000003666; FungiDB:YJL130C; VEuPathDB:YJL130C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables aspartate carbamoyltransferase activity and carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity. Involved in 'de novo' pyrimidine nucleobase biosynthetic process; glutamine metabolic process; and negative regulation of pyrimidine nucleobase metabolic process. Located in cytoplasm and membrane. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 50. Orthologous to human CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See URA2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (165723..172367, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutation or inhibition of Hsp90 resulted in decreased accumulation of Ura2, indicating it is an Hsp90 client. Cpr6 interacted with Ura2 in the absence of stable Cpr6-Hsp90 interaction, suggesting a direct interaction.
Title: Interaction of heat shock protein 90 and the co-chaperone Cpr6 with Ura2, a bifunctional enzyme required for pyrimidine biosynthesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables amino acid binding	IEA Inferred from Electronic Annotation more info
enables aspartate carbamoyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables aspartate carbamoyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables aspartate carbamoyltransferase activity	IEA Inferred from Electronic Annotation more info
enables aspartate carbamoyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables carbamoyl-phosphate synthase (ammonia) activity	IEA Inferred from Electronic Annotation more info
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables glutaminase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutamine metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of pyrimidine nucleobase metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in pyrimidine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in small molecule metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase

NP_012405.2

EC 2.1.3.2

EC 3.5.1.2

EC 6.3.4.16

EC 6.3.5.5

Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

165723..172367 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181563.2 → NP_012405.2 TPA: bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012405.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A2TBN0, D6VW55, P07259, Q06HN1

UniProtKB/TrEMBL

B3LPY8, N1P1J1

Conserved Domains (4) summary

COG0540
Location:1904 → 2212

PyrB; Aspartate carbamoyltransferase, catalytic chain [Nucleotide transport and metabolism]

TIGR01368
Location:23 → 409

CPSaseIIsmall; carbamoyl-phosphate synthase, small subunit

TIGR01369
Location:439 → 1485

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

cl00281
Location:1503 → 1830

metallo-dependent_hydrolases; Superfamily of metallo-dependent hydrolases (also called amidohydrolase superfamily) is a large group of proteins that show conservation in their 3-dimensional fold (TIM barrel) and in details of their active site. The vast majority of the members have a ...