Marchf8 membrane associated ring-CH-type finger 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Marchf8provided by MGI
Official Full Name: membrane associated ring-CH-type finger 8provided by MGI
Primary source: MGI:MGI:1919029
See related: Ensembl:ENSMUSG00000025702 AllianceGenome:MGI:1919029
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mir; March8; MARCH-VIII; 1300017E09Rik
Summary: The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Expression: Ubiquitous expression in kidney adult (RPKM 18.7), liver E14 (RPKM 18.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 E3; 6 53.78 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (116312546..116386501)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (116337599..116409540)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
Title: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
Title: MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Title: MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
CD83-mediated MHCII stabilization through antagonism of March8 as a novel functional adaptation of cortical epithelial cells for T cell selection.
Title: Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83.
orchestrated regulation of MHC II surface expression in thymic epithelial cells by MARCH 8 and CD83 plays a major role in CD4(+) T cell selection.
Title: Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MHC class II protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables MHC protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in adaptive immune response	IEA Inferred from Electronic Annotation more info
involved_in antigen processing and presentation of peptide antigen via MHC class II	IBA Inferred from Biological aspect of Ancestor more info
involved_in antigen processing and presentation of peptide antigen via MHC class II	IDA Inferred from Direct Assay more info	PubMed
involved_in immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of MHC class II biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in early endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
is_active_in late endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info
is_active_in lysosome	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in lysosome	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase MARCHF8

Names: E3 ubiquitin-protein ligase MARCH8; RING-type E3 ubiquitin transferase MARCH8; RING-type E3 ubiquitin transferase MARCHF8; c-mir, cellular modulator of immune recognition; cellular modulator of immune recognition; membrane-associated RING finger protein 8; membrane-associated RING-CH protein VIII; membrane-associated ring finger (C3HC4) 8

NP_001289312.1

EC 2.3.2.27

NP_001289313.1

EC 2.3.2.27

NP_001289314.1

EC 2.3.2.27

NP_001388597.1

EC 2.3.2.27

NP_001388598.1

EC 2.3.2.27

NP_001388599.1

EC 2.3.2.27

NP_001388600.1

EC 2.3.2.27

NP_001388601.1

EC 2.3.2.27

NP_001388602.1

EC 2.3.2.27

NP_082196.1

EC 2.3.2.27

XP_006506704.1

EC 2.3.2.27

XP_006506705.1

EC 2.3.2.27

XP_011239771.1

EC 2.3.2.27

XP_036008204.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001302383.2 → NP_001289312.1 E3 ubiquitin-protein ligase MARCHF8 isoform 1

See identical proteins and their annotated locations for NP_001289312.1

Status: REVIEWED

Source sequence(s)

AC123548

Consensus CDS

CCDS20451.1

UniProtKB/Swiss-Prot

Q9DBD2

Related

ENSMUSP00000078024.7, ENSMUST00000079012.13

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001302384.2 → NP_001289313.1 E3 ubiquitin-protein ligase MARCHF8 isoform 1

See identical proteins and their annotated locations for NP_001289313.1

Status: REVIEWED

Source sequence(s)

AC123548

Consensus CDS

CCDS20451.1

UniProtKB/Swiss-Prot

Q9DBD2

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001302385.2 → NP_001289314.1 E3 ubiquitin-protein ligase MARCHF8 isoform 2

See identical proteins and their annotated locations for NP_001289314.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AC123548

Consensus CDS

CCDS85138.1

UniProtKB/TrEMBL

A0A0N4SVK0, F6THH9

Related

ENSMUSP00000145137.2, ENSMUST00000203193.3

Conserved Domains (1) summary

cd16698
Location:36 → 87

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
NM_001401668.1 → NP_001388597.1 E3 ubiquitin-protein ligase MARCHF8 isoform 3

Status: REVIEWED

Source sequence(s)

AC123548

UniProtKB/TrEMBL

A0A0N4SV35

Related

ENSMUSP00000144936.2, ENSMUST00000123405.8
NM_001401669.1 → NP_001388598.1 E3 ubiquitin-protein ligase MARCHF8 isoform 4

Status: REVIEWED

Source sequence(s)

AC123548
NM_001401670.1 → NP_001388599.1 E3 ubiquitin-protein ligase MARCHF8 isoform 3

Status: REVIEWED

Source sequence(s)

AC123548

UniProtKB/TrEMBL

A0A0N4SV35
NM_001401671.1 → NP_001388600.1 E3 ubiquitin-protein ligase MARCHF8 isoform 5

Status: REVIEWED

Source sequence(s)

AC123548
NM_001401672.1 → NP_001388601.1 E3 ubiquitin-protein ligase MARCHF8 isoform 6

Status: REVIEWED

Source sequence(s)

AC123548

Related

ENSMUSP00000115510.3, ENSMUST00000135901.2
NM_001401673.1 → NP_001388602.1 E3 ubiquitin-protein ligase MARCHF8 isoform 2

Status: REVIEWED

Source sequence(s)

AC123548

UniProtKB/TrEMBL

A0A0N4SVK0
NM_027920.6 → NP_082196.1 E3 ubiquitin-protein ligase MARCHF8 isoform 1

See identical proteins and their annotated locations for NP_082196.1

Status: REVIEWED

Source sequence(s)

AC123548

Consensus CDS

CCDS20451.1

UniProtKB/Swiss-Prot

Q9DBD2

Related

ENSMUSP00000098594.4, ENSMUST00000101032.10

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

116312546..116386501

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011241469.3 → XP_011239771.1 E3 ubiquitin-protein ligase MARCHF8 isoform X3

See identical proteins and their annotated locations for XP_011239771.1

Conserved Domains (1) summary

cd16698
Location:318 → 369

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
XM_006506641.5 → XP_006506704.1 E3 ubiquitin-protein ligase MARCHF8 isoform X1

See identical proteins and their annotated locations for XP_006506704.1

UniProtKB/TrEMBL

A0A0N4SV35

Conserved Domains (1) summary

cd16698
Location:357 → 408

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
XM_036152311.1 → XP_036008204.1 E3 ubiquitin-protein ligase MARCHF8 isoform X4

UniProtKB/Swiss-Prot

Q9DBD2

Conserved Domains (1) summary

cd16698
Location:75 → 126

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
XM_006506642.2 → XP_006506705.1 E3 ubiquitin-protein ligase MARCHF8 isoform X1

See identical proteins and their annotated locations for XP_006506705.1

UniProtKB/TrEMBL

A0A0N4SV35

Conserved Domains (1) summary

cd16698
Location:357 → 408

RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins