Slc4a2 solute carrier family 4 member 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc4a2provided by RGD
Official Full Name: solute carrier family 4 member 2provided by RGD
Primary source: RGD:3711
See related: EnsemblRapid:ENSRNOG00000014347 AllianceGenome:RGD:3711
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Ae2; Aep2
Summary: Enables chloride:bicarbonate antiporter activity and enzyme binding activity. Involved in digestive tract development; regulation of intracellular pH; and spermatogenesis. Located in apical plasma membrane and basolateral plasma membrane. Biomarker of autosomal recessive polycystic kidney disease and prediabetes syndrome. Human ortholog(s) of this gene implicated in osteopetrosis. Orthologous to human SLC4A2 (solute carrier family 4 member 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 163.6), Kidney (RPKM 113.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q11

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (11628860..11646961, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (10736419..10754407, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (7264677..7282355, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicate that the increase in bile flow induced by rifampicin is mainly due to increased HCO3 (-) excretion mediated by increased AE2 protein expression and activity.
Title: Rifampicin Induces Bicarbonate-Rich Choleresis in Rats: Involvement of Anion Exchanger 2.
Results suggest that exhaustive running exercise results in elevated band-3 tyrosine phosphorylation and alters band-3 membrane organization.
Title: Exhaustive running exercise induce tyrosine phosphorylation of band 3 in rat erythrocytes.
These results demonstrate that NHE1 and AE2 are important regulators of bone growth.
Title: Suppression of mammalian bone growth by membrane transport inhibitors.
Data suggest that hepatic cystogenesis in autosomal recessive polycystic kidney disease may involve increased fluid accumulation because of overexpression and abnormal location of AQP1, CFTR, and AE2 in cystic cholangiocytes.
Title: Hepatic cystogenesis is associated with abnormal expression and location of ion transporters and water channels in an animal model of autosomal recessive polycystic kidney disease.
Coexpression of carbonic anhydrase IX and SLC4A2 activated SLC4A2 mediated transport.
Title: Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters.
Immunohistochemical localization of proteins known to be important in HCO(3) (-) secretion was investigated in the rat common bile duct.
Title: Bicarbonate secretion by rat bile duct brush cells indicated by immunohistochemical localization of CFTR, anion exchanger AE2, Na+/HCO3 -cotransporter, carbonic anhydrase II, Na+/H+ exchangers NHE1 and NHE3, H+/K+-ATPase, and Na+/K+-ATPase.
the regulation following NH(4)Cl loading of AE2b in the cortical thick ascending limb suggests a role for AE2 in transepithelial bicarbonate reabsorption in this segment
Title: AE2 isoforms in rat kidney: immunohistochemical localization and regulation in response to chronic NH4Cl loading.
regulation of the basolateral Cl(-)/HCO(3)(-) exchanger AE2 plays an important role in the adaptation of bicarbonate absorption in the thick ascending loop of Henle during chronic acid-base disturbances and high sodium intake.
Title: Regulation of the Cl-/HCO3- exchanger AE2 in rat thick ascending limb of Henle's loop in response to changes in acid-base and sodium balance.
Hepatocytic apical targeting of anion exchanger 2 (AE2) isoforms suggests that they all may participate in the canalicular secretion of bicarbonate to bile.
Title: Shared apical sorting of anion exchanger isoforms AE2a, AE2b1, and AE2b2 in primary hepatocytes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc4a2 (e-PCR), detects polymorphism
- UniSTS:547349

D4Bro1 (e-PCR)
- UniSTS:240413

d4bro1 (e-PCR)
- UniSTS:120430

D4Wox29 (e-PCR)
- UniSTS:518864

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables chloride transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables chloride:bicarbonate antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables chloride:bicarbonate antiporter activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables monoatomic anion transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables solute:inorganic anion antiporter activity	IEA Inferred from Electronic Annotation more info
enables transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in amelogenesis	ISO Inferred from Sequence Orthology more info
involved_in bicarbonate transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in chloride transport	ISO Inferred from Sequence Orthology more info
involved_in digestive tract development	IEP Inferred from Expression Pattern more info	PubMed
involved_in inorganic anion transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic anion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of CD8-positive, alpha-beta T cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of CD8-positive, alpha-beta T cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of CD8-positive, alpha-beta T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of CD8-positive, alpha-beta T cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in osteoclast differentiation	ISO Inferred from Sequence Orthology more info
involved_in osteoclast differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of enamel mineralization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of bone resorption	ISO Inferred from Sequence Orthology more info
involved_in regulation of bone resorption	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of intracellular pH	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of intracellular pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in spermatogenesis	ISO Inferred from Sequence Orthology more info
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in basolateral plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: anion exchange protein 2

Names: AE 2; AE2 Cl-/HCO3-exchanger, variant AE2b; B3RP-2; Solute carrier family 4, member 2, anion exchange protein 2; anion exchanger 2; band 3-related protein 2; non-erythroid band 3-like protein; solute carrier family 4 (anion exchanger), member 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017048.2 → NP_058744.1 anion exchange protein 2

See identical proteins and their annotated locations for NP_058744.1

Status: PROVISIONAL

Source sequence(s)

J05166

UniProtKB/Swiss-Prot

P23347

UniProtKB/TrEMBL

A0A8I6AM83, A6K551

Related

ENSRNOP00000019666.2, ENSRNOT00000019666.4

Conserved Domains (1) summary

TIGR00834
Location:321 → 1233

ae; anion exchange protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

11628860..11646961 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006235874.5 → XP_006235936.1 anion exchange protein 2 isoform X1

See identical proteins and their annotated locations for XP_006235936.1

UniProtKB/Swiss-Prot

P23347

UniProtKB/TrEMBL

A0A8I6AM83, A6K551

Conserved Domains (1) summary

TIGR00834
Location:321 → 1233

ae; anion exchange protein
XM_039107064.2 → XP_038962992.1 anion exchange protein 2 isoform X1

UniProtKB/Swiss-Prot

P23347

UniProtKB/TrEMBL

A0A8I6AM83, A6K551

Conserved Domains (1) summary

TIGR00834
Location:321 → 1233

ae; anion exchange protein
XM_006235875.5 → XP_006235937.1 anion exchange protein 2 isoform X1

See identical proteins and their annotated locations for XP_006235937.1

UniProtKB/Swiss-Prot

P23347

UniProtKB/TrEMBL

A0A8I6AM83, A6K551

Conserved Domains (1) summary

TIGR00834
Location:321 → 1233

ae; anion exchange protein
XM_039107065.2 → XP_038962993.1 anion exchange protein 2 isoform X1

UniProtKB/Swiss-Prot

P23347

UniProtKB/TrEMBL

A0A8I6AM83, A6K551

Conserved Domains (1) summary

TIGR00834
Location:321 → 1233

ae; anion exchange protein
XM_006235877.5 → XP_006235939.1 anion exchange protein 2 isoform X2

UniProtKB/TrEMBL

A0A8I5Y5U0, A0A8I6AM83

Related

ENSRNOP00000077442.1, ENSRNOT00000111245.2

Conserved Domains (3) summary

TIGR00834
Location:312 → 1224

ae; anion exchange protein

pfam00955
Location:663 → 1151

HCO3_cotransp; HCO3- transporter family

pfam07565
Location:347 → 608

Band_3_cyto; Band 3 cytoplasmic domain