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    csgD DNA-binding transcriptional dual regulator CsgD [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949119, updated on 3-Dec-2024

    Summary

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    Official Symbol
    csgD
    Official Full Name
    DNA-binding transcriptional dual regulator CsgD
    Primary source
    ECOCYC:G6546
    Locus tag
    b1040
    See related
    ASAP:ABE-0003530
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1026
    Summary
    CsgD (AgfD) has also been characterized in Salmonella. [More information is available at EcoGene: EG13410]. The protein CsgD, for "Curlin subunit gene D," is a transcriptional regulator that regulates a number of genes involved in the Curli assembly, transport, and structural components , which are important for biofilm formation . [More information is available at EcoCyc: G6546].
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    Genomic context

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    Sequence:
    NC_000913.3 (1102546..1103196, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene curli assembly component CsgF Neighboring gene curli assembly component CsgE Neighboring gene curlin, minor subunit Neighboring gene curlin, major subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12. Ogasawara H, et al. Microbiology (Reading), 2020 Sep. PMID 32649279
    2. The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli. Smith DR, et al. Biomolecules, 2017 Oct 31. PMID 29088115, Free PMC Article
    3. Genome amplification and promoter mutation expand the range of csgD-dependent biofilm responses in an STEC population. Uhlich GA, et al. Microbiology (Reading), 2017 Apr. PMID 28406080
    4. Complex regulation of csgD promoter activity by global regulatory proteins. Gerstel U, et al. Mol Microbiol, 2003 Aug. PMID 12864849
    5. Regulation of the Escherichia coli csgD promoter: interplay between five transcription factors. Ogasawara H, et al. Microbiology (Reading), 2010 Aug. PMID 20466769

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12.
      Title: Novel regulators of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli K-12.
    2. A conserved A/U-rich region in the csgD mRNA 5' untranslated region, which is cleaved upon binding of the small RNAs, McaS, RprA or GcvB, to sites located more than 30 nucleotides downstream.
      Title: sRNA-dependent control of curli biosynthesis in Escherichia coli: McaS directs endonucleolytic cleavage of csgD mRNA.
    3. Regulation of the master biofilm regulator, CsgD, was diverse, and the screen revealed several proteins and small RNAs (sRNA) that regulate csgD messenger RNA (mRNA) levels. Using previously published studies, we found minimal overlap between the genes affecting curli biogenesis and genes known to impact swimming or swarming motility, underlying the distinction between motile and sessile lifestyles.
      Title: The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coli.
    4. Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli.
      Title: Influence of csgD and ompR on Nanomechanics, Adhesion Forces, and Curli Properties of E. coli.
    5. Promoter mutations in csgD that alter sigma factor utilization from RpoS to RpoD provide a unique mechanism for increasing biofilm formation, but the transition also disrupts normal rpoS-dependent CsgD regulation.
      Title: Genome amplification and promoter mutation expand the range of csgD-dependent biofilm responses in an STEC population.
    6. cAMP and CRP influence E. coli biofilms through transcriptional regulation of csgD.
      Title: The Catabolite Repressor Protein-Cyclic AMP Complex Regulates csgD and Biofilm Formation in Uropathogenic Escherichia coli.
    7. comparison of expression of genes involved in folate metabolism and the hmp gene in strains with intact csgD gene and with deletion in csgD; expression of genes for methylene tetrahydrofolate synthesis and formyltetrahydrofolate recycling was higher in cells with CsgD than those without; expression of hmp was lower in the presence of CsgD
      Title: The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12.
    8. In conclusion, Hha regulates biofilm formation in enterohemorrhagic Escherichia coli O157:H7 by differential regulation of FlhDC and CsgD, the global regulators of motility and curli production, respectively.
      Title: Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD.
    9. RprA can directly reduce csgD mRNA levels and translation, and ce transcription of csgD.
      Title: Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.
    10. The authors propose that the cell motility for planktonic growth is repressed by CsgD, thereby promoting the switch to biofilm formation.
      Title: Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription activator activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables DNA-binding transcription repressor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in negative regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of DNA-templated transcription initiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of single-species biofilm formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-DNA complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA-binding transcriptional dual regulator CsgD

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1102546..1103196 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415558.1 DNA-binding transcriptional dual regulator CsgD [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415558.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6Q3S0
      Conserved Domains (1) summary
      PRK10100
      Location:1 → 216
      PRK10100; transcriptional regulator CsgD
    Nucleotide Protein
    Heading Accession and Version

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