gadB glutamate decarboxylase B [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: gadB
Official Full Name: glutamate decarboxylase B
Primary source: ECOCYC:EG11490
Locus tag: b1493
See related: ASAP:ABE-0004976
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1487
Summary: RpoS regulon. [More information is available at EcoGene: EG11490]. GadB, a glutamate decarboxylase enzyme, is part of the glutamate-dependent acid resistance system 2 (AR2) which confers resistance to extreme acid conditions. [More information is available at EcoCyc: EG11490].
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Genomic context

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Sequence:: NC_000913.3 (1570645..1572045, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Small angle X-ray scattering, combined with size exclusion chromatography, and analytical ultracentrifugation analysis show that the compact and entangled EcGadB hexameric structure undergoes dissociation into dimers as pH alkalinizes.
Title: On the effect of alkaline pH and cofactor availability in the conformational and oligomeric state of Escherichia coli glutamate decarboxylase.
Data show that Corynebacterium glutamicum expressing E. coli glutamate decarboxylase (GAD) mutant under the strong PH36 promoter could produce GABA 17-fold higher than that obtained by C. glutamicum expressing wild-type E. coli GAD.
Title: Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range.
During anaerobic growth at pH 5.5, strains deleted for fnr showed enhanced transcription of acid-survival genes gadB, cfa, and hdeA, as well as catalase (katE).
Title: Escherichia coli K-12 survives anaerobic exposure at pH 2 without RpoS, Gad, or hydrogenases, but shows sensitivity to autoclaved broth products.
Mutation of His465 alters the pH-dependent spectroscopic properties of Escherichia coli glutamate decarboxylase and broadens the range of its activity toward more alkaline pH.
Title: Mutation of His465 alters the pH-dependent spectroscopic properties of Escherichia coli glutamate decarboxylase and broadens the range of its activity toward more alkaline pH.
N-terminus verified by Edman degradation on complete protein
Title: Function of the Escherichia coli nucleoid protein, H-NS: molecular analysis of a subset of proteins whose expression is enhanced in a hns deletion mutant.
Results show that chloride is an allosteric activator of GadB.
Title: Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables carboxy-lyase activity	IEA Inferred from Electronic Annotation more info
enables glutamate decarboxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate decarboxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate decarboxylase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables pyridoxal phosphate binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glutamate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamate metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within intracellular pH elevation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: glutamate decarboxylase B

NP_416010.1

EC 4.1.1.15

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1570645..1572045 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416010.1 glutamate decarboxylase B [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416010.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A2X1NFS6, A0A8S7CSP5, A5YKJ3, A5YKJ6, A5YKJ8

Conserved Domains (1) summary

TIGR01788
Location:16 → 449

Glu-decarb-GAD; glutamate decarboxylase