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    menF isochorismate synthase MenF [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946712, updated on 3-Dec-2024

    Summary

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    Official Symbol
    menF
    Official Full Name
    isochorismate synthase MenF
    Primary source
    ECOCYC:EG12362
    Locus tag
    b2265
    See related
    ASAP:ABE-0007485
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2259; yfbA
    Summary
    There are two isochorismate synthase enzymes present in Escherichia coli, encoded by menF and entC. [More information is available at EcoCyc: EG12362].
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    Genomic context

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    Sequence:
    NC_000913.3 (2379348..2380643, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene o-succinylbenzoate synthase Neighboring gene 1,4-dihydroxy-2-naphthoyl-CoA synthase Neighboring gene 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Neighboring gene 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase Neighboring gene tail-anchored inner membrane protein ElaB Neighboring gene putative acetyltransferase ElaA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure of isochorismate synthase in complex with magnesium. Parsons JF, et al. Acta Crystallogr D Biol Crystallogr, 2008 May. PMID 18453696, Free PMC Article
    2. Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family. Kolappan S, et al. Biochemistry, 2007 Jan 30. PMID 17240978
    3. Clustering of isochorismate synthase genes menF and entC and channeling of isochorismate in Escherichia coli. Buss K, et al. Biochim Biophys Acta, 2001 Dec 30. PMID 11779629
    4. Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli. Daruwala R, et al. J Bacteriol, 1997 May. PMID 9150206, Free PMC Article
    5. A new isochorismate synthase specifically involved in menaquinone (vitamin K2) biosynthesis encoded by the menF gene. Daruwala R, et al. FEMS Microbiol Lett, 1996 Jul 1. PMID 8764478

    See all (16) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Lys190 has been identified as the base that activates water for nucleophilic attack at the chorismate C2 carbon.
      Title: Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables isochorismate synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables isochorismate synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables isochorismate synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in menaquinone biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in menaquinone biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within menaquinone biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    isochorismate synthase MenF
    NP_416768.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2379348..2380643 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416768.4 isochorismate synthase MenF [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416768.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FZE5
      Conserved Domains (1) summary
      PRK15012
      Location:1 → 431
      PRK15012; menaquinone-specific isochorismate synthase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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