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    leuB 3-isopropylmalate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944798, updated on 17-Dec-2024

    Summary

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    Official Symbol
    leuB
    Official Full Name
    3-isopropylmalate dehydrogenase
    Primary source
    ECOCYC:EG11577
    Locus tag
    b0073
    See related
    ASAP:ABE-0000265
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0075
    Summary
    3-isopropylmalate dehydrogenase (LeuB) carries out the third step in leucine biosynthesis, catalyzing the conversion of 3-isopropylmalate to 2-isopropyl-3-oxosuccinate. [More information is available at EcoCyc: EG11577].
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    Genomic context

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    Sequence:
    NC_000913.3 (80867..81958, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-isopropylmalate dehydratase subunit LeuD Neighboring gene 3-isopropylmalate dehydratase subunit LeuC Neighboring gene 2-isopropylmalate synthase Neighboring gene leu operon leader peptide

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus. Wallon G, et al. J Mol Biol, 1997 Mar 14. PMID 9086278
    2. Pervasive cryptic epistasis in molecular evolution. Lunzer M, et al. PLoS Genet, 2010 Oct 21. PMID 20975933, Free PMC Article
    3. A link between hinge-bending domain motions and the temperature dependence of catalysis in 3-isopropylmalate dehydrogenase. Hajdú I, et al. Biophys J, 2009 Jun 17. PMID 19527660, Free PMC Article
    4. Rates of unfolding, rather than refolding, determine thermal stabilities of thermophilic, mesophilic, and psychrotrophic 3-isopropylmalate dehydrogenases. Gráczer E, et al. Biochemistry, 2007 Oct 16. PMID 17887729
    5. Hypermutation in derepressed operons of Escherichia coli K12. Wright BE, et al. Proc Natl Acad Sci U S A, 1999 Apr 27. PMID 10220423, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on d-malate.
      Title: Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on d-malate.
    2. Data show that residues that affect function are scattered haphazardly throughout the IMDH structure.
      Title: Pervasive cryptic epistasis in molecular evolution.
    3. N-terminus verified by Edman degradation on mature peptide
      Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3-isopropylmalate dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3-isopropylmalate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3-isopropylmalate dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables manganese ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in L-leucine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within L-leucine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in L-leucine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within L-leucine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to amino acid starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol EXP
    Inferred from Experiment
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    3-isopropylmalate dehydrogenase
    NP_414615.4

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      80867..81958 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414615.4 3-isopropylmalate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414615.4

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3JNC7
      Conserved Domains (1) summary
      PRK00772
      Location:3 → 362
      PRK00772; 3-isopropylmalate dehydrogenase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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